C57BL/6JCya-Dab2em1/Cya
Common Name:
Dab2-KO
Product ID:
S-KO-20603
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Dab2-KO
Strain ID
KOCMP-13132-Dab2-B6J-VA
Gene Name
Product ID
S-KO-20603
Gene Alias
5730435J12Rik; D15Wsu122e; D630005B22Rik; Doc-2; Doc2; p96
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
15
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dab2em1/Cya mice (Catalog S-KO-20603) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000080880
NCBI RefSeq
NM_023118
Target Region
Exon 3~4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Dab2, also known as Disabled-2 or DOC-2, is a mitogen-responsive adaptor protein essential for multiple cellular functions. It plays a crucial role in clathrin-mediated endocytosis and is involved in key signalling pathways such as mitogen-activated protein kinase (MAPK), wingless/integrated (Wnt), and transforming growth factor beta (TGFβ) pathways, which are related to homeostasis, cell positioning, and epithelial to mesenchymal transition (EMT) [1,2].
In a Dab2 conditional knockout (Dab2-cKO) mouse model, the hair follicle (HF) cycle was delayed due to perturbed activation of HF stem cells (HFSCs). The number of HFSCs and their colony-forming ability were reduced. The HFSCs in Dab2-cKO mice showed extended quiescence, and the expression of anti-aging genes decreased, suggesting an accelerated aging process. This reveals the role of Dab2 in regulating the activation and self-renewal of HFSCs [3].
In another study, knockdown of DAB2 in human lung fibroblast MRC-5 cells treated with TGF-β1 suppressed cell proliferation and the expression of fibrosis-related proteins. This indicates that DAB2 may play a role in promoting pulmonary fibrosis [4].
In conclusion, Dab2 is a multifunctional protein involved in various cellular functions and signalling pathways. Model-based research, especially the Dab2-cKO mouse model, has revealed its role in processes like HFSC self-renewal and in disease conditions such as pulmonary fibrosis. These findings contribute to understanding the biological functions of Dab2 and its potential implications in disease development.
References:
1. Price, Zoe K, Lokman, Noor A, Yoshihara, Masato, Oehler, Martin K, Ricciardelli, Carmela. 2022. Disabled-2 (DAB2): A Key Regulator of Anti- and Pro-Tumorigenic Pathways. In International journal of molecular sciences, 24, . doi:10.3390/ijms24010696. https://pubmed.ncbi.nlm.nih.gov/36614139/
2. Ogbu, Stella C, Musich, Phillip R, Zhang, Jinyu, Howe, Philip H, Jiang, Yong. 2020. The role of disabled-2 (Dab2) in diseases. In Gene, 769, 145202. doi:10.1016/j.gene.2020.145202. https://pubmed.ncbi.nlm.nih.gov/33059028/
3. Roy, Sayoni, Mehta, Darshan, Paradkar, Akshay, Chovatiya, Gopal, Waghmare, Sanjeev K. 2024. Dab2 (Disabled-2), an adaptor protein, regulates self-renewal of hair follicle stem cells. In Communications biology, 7, 525. doi:10.1038/s42003-024-06047-2. https://pubmed.ncbi.nlm.nih.gov/38702433/
4. Liang, Chun-Lian, Li, Xiu-Li, Quan, Xiao-Juan, Zhang, Lin. 2023. DAB2 promotes pulmonary fibrosis and may act as an intermediate between IGF‑1R and PI3K/AKT signaling pathways. In Experimental and therapeutic medicine, 25, 183. doi:10.3892/etm.2023.11882. https://pubmed.ncbi.nlm.nih.gov/37021069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen