C57BL/6JCya-Mboat2em1/Cya
Common Name
Mboat2-KO
Product ID
S-KO-20848
Backgroud
C57BL/6JCya
Strain ID
KOCMP-67216-Mboat2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Mboat2-KO Mouse (Catalog S-KO-20848) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Mboat2-KO
Strain ID
KOCMP-67216-Mboat2-B6J-VB
Gene Name
Product ID
S-KO-20848
Gene Alias
Oact2, LPCAT4, Moact2, 2810049G06Rik
Background
C57BL/6JCya
Gene Full Name
membrane bound O-acyltransferase domain containing 2
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 12 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000110942
NCBI Transcript ID
NM_026037
Target Region
Exon 7~8
Size of Effective Region
~2.7 kb
Overview of Gene Research
Mboat2, membrane bound O-acyltransferase domain containing 2, is a phospholipid-modifying enzyme. It has been identified to play roles in multiple biological processes. Mboat2 can regulate lipid metabolism, and is also involved in ferroptosis surveillance, cell-cell communication, cell cycling, and immune-related biological functions such as leukocyte activation in T-cell-receptor signaling pathway, inflammatory response, and antigen processing and presentation [2,5].
In pancreatic cancer, Mboat2 overexpression accelerates cell proliferation and migration, enhances CDK2 and CCNA2 expression for cell cycle progression, and reduces CD8+ T-cell infiltration [5]. In intrahepatic cholangiocarcinoma (ICC), the circRNA derived from Mboat2 (circMBOAT2) promotes ICC progression and lipid metabolism reprogramming by stabilizing PTBP1 to facilitate FASN mRNA cytoplasmic export [1]. In coronary chronic total occlusion (CTO), circ-MBOAT2 regulates angiogenesis via the miR-495/NOTCH1 axis and associates with myocardial perfusion [3]. In pancreatic cancer, circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis [4].
In summary, Mboat2 and its derived circRNA play crucial roles in various diseases, especially in cancers. Studies on Mboat2-related KO/CKO mouse models or other loss-of-function experiments have helped to reveal its functions in promoting tumor progression, regulating lipid metabolism, angiogenesis, and ferroptosis surveillance, providing potential therapeutic targets for these diseases [1,2,3,4,5].
References：
1. Yu, Xiaopeng, Tong, Huanjun, Chen, Jialu, Wang, Shouhua, Tang, Zhaohui. 2023. CircRNA MBOAT2 promotes intrahepatic cholangiocarcinoma progression and lipid metabolism reprogramming by stabilizing PTBP1 to facilitate FASN mRNA cytoplasmic export. In Cell death & disease, 14, 20. doi:10.1038/s41419-022-05540-y. https://pubmed.ncbi.nlm.nih.gov/36635270/
2. Liang, Deguang, Feng, Yan, Zandkarimi, Fereshteh, Stockwell, Brent R, Jiang, Xuejun. 2023. Ferroptosis surveillance independent of GPX4 and differentially regulated by sex hormones. In Cell, 186, 2748-2764.e22. doi:10.1016/j.cell.2023.05.003. https://pubmed.ncbi.nlm.nih.gov/37267948/
3. Gao, Wei, Li, Chenguang, Yuan, Jie, Liu, Haibo, Ge, Junbo. 2024. Circ-MBOAT2 Regulates Angiogenesis via the miR-495/NOTCH1 Axis and Associates with Myocardial Perfusion in Patients with Coronary Chronic Total Occlusion. In International journal of molecular sciences, 25, . doi:10.3390/ijms25020793. https://pubmed.ncbi.nlm.nih.gov/38255868/
4. Zhou, Xiaoxiao, Liu, Kun, Cui, Jing, Peng, Tao, Guo, Yao. 2021. Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer. In Journal of experimental & clinical cancer research : CR, 40, 124. doi:10.1186/s13046-021-01894-x. https://pubmed.ncbi.nlm.nih.gov/33832516/
5. Li, Zhenchong, Zhuang, Hongkai, Chen, Xinming, Zhang, Chuanzhao, Hou, Baohua. 2022. Identification of MBOAT2 as an Unfavorable Biomarker Correlated with KRAS Activation and Reduced CD8+ T-Cell Infiltration in Pancreatic Cancer. In Journal of oncology, 2022, 4269733. doi:10.1155/2022/4269733. https://pubmed.ncbi.nlm.nih.gov/35571489/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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