Knockout Cell Lines
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Using CRISPR/Cas9 technology, we disable the gene of interest by the removal of large fragments of the gene instead of the introduction of a frameshift mutation, which will make sure the complete loss of gene function. Cyagen is the world`s leading provider of custom genome editing services. Leverage our experts and optimized CRISPR/Cas9 cell line modeling service platform CRISPR-Pro for a hassle-free research. CRISPR-Pro enables large fragments excision or correct mutations in various cell lines.
◆ Workflow
◆ Variety of cell lines
Cancer cells, hard-to-transfect cells, stem cells and more.
◆ Advantages of large fragment knockout
Our CRISPR-Pro technology can bring paired gRNAs by which large fragment deletion can be obtained. Compared with frameshift mutations, the removal of large fragments of the gene is more efficient and straight-forward.
| Comparison |
Large fragment KO |
Frameshift mutation | ||
| Plasmid electroporation |
Lentivirus transduction |
Plasmid electroporation | Lentivirus transduction | |
| Outcomes |
Complete deletion of large fragment enables inactivation for all transcripts, much likely including unknown transcripts as well. |
Transcripts from downstream and some unknown transcripts may not be affected. | ||
| Deletion Limit |
Large fragments |
One or several base pairs | ||
| Identification |
Easy by PCR assays |
Sequencing needed |
||
| If Virus Packaging Needed | No |
Yes |
No | Yes |
| Turnaround | 2~3 months |
3~5 months |
2~3 months | 3~5 months |
◆ Why work with Cyagen?
- Guaranteed: 100% money back guarantee.
- Reliable: Complete loss of gene function.
- Short timeline: As fast as 2 months.
- Substantial experience: Over 13 yearsof experience with thousands of gene editing projects delivered.
- Optimized technology: By using optimized CRISPR/Cas9 technology CRISPR-Pro, the removal of large fragments of the gene can be achieved.
- Customer citations: Over 2,400 publications citing our services and products.
◆ Inquiries and quote requests
Request a quote now. Alternatively, you can always email cell-service@cyagen.com to inquire about our services or obtain a quote for your project.
