Knockout Cell Lines

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Knockout Cell Lines
Using CRISPR/Cas9 technology, we disable the gene of interest by the removal of large fragments of the gene instead of the introduction of a frameshift mutation, which will make sure the complete loss of gene function. Cyagen is the world`s leading provider of custom genome editing services. Leverage our experts and optimized CRISPR/Cas9 cell line modeling service platform CRISPR-Pro for a hassle-free research. CRISPR-Pro enables large fragments excision or correct mutations in various cell lines.
◆ Workflow

 

◆ Variety of cell lines

Cancer cells, hard-to-transfect cells, stem cells and more.

 

◆ Advantages of large fragment knockout

Our CRISPR-Pro technology can bring paired gRNAs by which large fragment deletion can be obtained. Compared with frameshift mutations, the removal of large fragments of the gene is more efficient and straight-forward.

Comparison

Large fragment KO

 Frameshift mutation
Plasmid electroporation

Lentivirus transduction

 Plasmid electroporation Lentivirus transduction
Outcomes

Complete deletion of large fragment enables inactivation for all transcripts, much likely including unknown transcripts as well.

 Transcripts from downstream and some unknown transcripts may not be affected.
Deletion Limit

Large fragments

 One or several base pairs
Identification

Easy by PCR assays

Sequencing needed
If Virus Packaging Needed No

Yes

 No Yes
Turnaround 2~3 months

3~5 months

 2~3 months 3~5 months

 

 

◆ Why work with Cyagen?
  • Guaranteed: 100% money back guarantee.
  • Reliable: Complete loss of gene function.
  • Short timeline: As fast as 2 months.
  • Substantial experience: Over 13 yearsof experience with thousands of gene editing projects delivered.
  • Optimized technology: By using optimized CRISPR/Cas9 technology CRISPR-Pro, the removal of large fragments of the gene can be achieved.
  • Customer citations: Over 2,400 publications citing our services and products.

 

◆ Inquiries and quote requests

Request a quote now. Alternatively, you can always email cell-service@cyagen.com to inquire about our services or obtain a quote for your project.