Cyagen provides comprehensive model generation services for all your transgenic mouse, mouse embryo, and rat model needs. Contact us to perform your entire project - from initial strategy design and DNA vector construction, all the way through breeding – we deliver research-ready transgenic rodent models for guaranteed results.
|Regular Transgenic||PiggyBac Transgenic||ROSA26 Large-Fragment Knockin|
|Integration||Random, multicopy integration||Random, single copy per integration site||Single copy transgene targeted to ROSA26 safe harbor locus|
|Vector construction||Transgenic plasmid or BAC||Transgenic plasmid or BAC||Targeting vector + gRNAs|
|Expression pattern||Variable expression in founders||More consistent expression in founders||Most consistent expression|
|Zygosity||Hemizygous||Hemizygous||Options: Heterozygous and Homo|
|Endogenous effects||Can disrupt endogenous gene expression||Less likely to disrupt endogenous gene expression||Safe harbor site (SHS) does not disrupt endogenous gene expression|
|Turnaround time||2-5 months||6-9 months|
|Donor background||Mouse strains: C57BL/6, FVB
Rat strains: Sprague-Dawley (SD), Long Evans
Please inquire for the availability of additional strains.
Our proprietary PiggyBac transgenic method has the following advantages over other transgenic approaches: