C57BL/6JCya-Zdhhc7em1flox/Cya
Common Name:
Zdhhc7-flox
Product ID:
S-CKO-00256
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zdhhc7-flox
Strain ID
CKOCMP-102193-Zdhhc7-B6J-VA
Gene Name
Product ID
S-CKO-00256
Gene Alias
Gramp2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zdhhc7em1flox/Cya mice (Catalog S-CKO-00256) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000034280
NCBI RefSeq
NM_133967
Target Region
Exon 4
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Zdhhc7, encoding zinc finger DHHC-type palmitoyl transferase 7, is involved in protein S-palmitoylation, a reversible post-translational modification. It plays significant roles in multiple biological pathways, including those related to inflammation, autophagy, and cell cycle regulation. Genetic models, such as Zdhhc7 knockout (KO) mice, are valuable tools for studying its functions [1,2,4-6].
In the context of inflammation, Zdhhc7-mediated palmitoylation of NLRP3 at Cys126 promotes inflammasome activation. Zdhhc7 KO reduces NLRP3 activation in macrophages, highlighting its importance in this inflammatory process [1].
In autophagy, Zdhhc7-mediated S-palmitoylation of ATG16L1 at Cys153 facilitates LC3 lipidation and autophagosome formation. ATG16L1-KO HeLa cells with S-palmitoylation-deficient mutant ATG16L1C153S show defects in these processes [2].
Regarding cell cycle and cancer, Zdhhc7 inhibits the androgen receptor in prostate cancer cells. Zdhhc7 depletion increases the oncogenic properties of prostate cancer cells, while restoring it suppresses cell proliferation and invasion in vitro and in vivo [3].
In a mouse model of colitis, pharmacological inhibition of APT2 or knockout of Zdhhc7 relieves symptoms, as Zdhhc7-mediated palmitoylation of STAT3 promotes TH17 cell differentiation [4].
In pyroptosis, Zdhhc7-mediated palmitoylation of GSDMD on C192 is crucial for its cleavage and subsequent pyroptosis-related events. Perturbing this palmitoylation suppresses pyroptosis, affecting mouse survival in lipopolysaccharide-induced septic shock [5].
In brain development, deleting the Zdhhc7 gene in mice impairs fibre development, demonstrating its role in microstructural changes [6].
In ovarian clear cell carcinoma, suppression of Zdhhc7 enhances ferroptosis via YAP1 activation [7].
In hepatocellular carcinoma, increased Zdhhc7 abundance is associated with poor prognosis, and perturbing the positive feedback loop among DHHC7, STAT3, and HIF1α reduces tumor cell growth in vivo [8].
In an Alzheimer's disease mouse model, hippocampal silencing of Zdhhc7 prevents cognitive deficits [9].
In non-alcoholic steatohepatitis, KLF10 promotes the disease progression through transcriptional activation of Zdhhc7, which palmitoylates CD36 [10].
In conclusion, Zdhhc7 is essential for various biological functions mainly through protein S-palmitoylation. Studies using Zdhhc7 KO or related genetic models have revealed its significance in multiple disease areas, including inflammatory diseases, cancer, neurodegenerative diseases, and metabolic liver diseases, providing potential therapeutic targets for these conditions.
References：
1. Yu, Tao, Hou, Dan, Zhao, Jiaqi, Linder, Maurine E, Lin, Hening. 2024. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. In Cell reports, 43, 114070. doi:10.1016/j.celrep.2024.114070. https://pubmed.ncbi.nlm.nih.gov/38583156/
2. Wei, Fujing, Wang, Yu, Yao, Jia, Kong, Eryan, Yang, Aimin. 2024. ZDHHC7-mediated S-palmitoylation of ATG16L1 facilitates LC3 lipidation and autophagosome formation. In Autophagy, 20, 2719-2737. doi:10.1080/15548627.2024.2386915. https://pubmed.ncbi.nlm.nih.gov/39087410/
3. Lin, Zhuoyuan, Agarwal, Shivani, Tan, Song, Zhao, Jonathan C, Yu, Jindan. 2023. Palmitoyl acyltransferase ZDHHC7 inhibits androgen receptor and suppresses prostate cancer. In Oncogene, 42, 2126-2138. doi:10.1038/s41388-023-02718-2. https://pubmed.ncbi.nlm.nih.gov/37198397/
4. Zhang, Mingming, Zhou, Lixing, Xu, Yuejie, Linder, Maurine E, Lin, Hening. 2020. A STAT3 palmitoylation cycle promotes TH17 differentiation and colitis. In Nature, 586, 434-439. doi:10.1038/s41586-020-2799-2. https://pubmed.ncbi.nlm.nih.gov/33029007/
5. Zhang, Na, Zhang, Jian, Yang, Yuanxin, Tan, Li, Xu, Daichao. 2024. A palmitoylation-depalmitoylation relay spatiotemporally controls GSDMD activation in pyroptosis. In Nature cell biology, 26, 757-769. doi:10.1038/s41556-024-01397-9. https://pubmed.ncbi.nlm.nih.gov/38538834/
6. Kerkenberg, Nicole, Wachsmuth, Lydia, Zhang, Mingyue, Zhang, Weiqi, Hohoff, Christa. 2021. Brain microstructural changes in mice persist in adulthood and are modulated by the palmitoyl acyltransferase ZDHHC7. In The European journal of neuroscience, 54, 5951-5967. doi:10.1111/ejn.15415. https://pubmed.ncbi.nlm.nih.gov/34355442/
7. Furutake, Yoko, Yamaguchi, Ken, Yamanoi, Koji, Matsumura, Noriomi, Mandai, Masaki. . YAP1 Suppression by ZDHHC7 Is Associated with Ferroptosis Resistance and Poor Prognosis in Ovarian Clear Cell Carcinoma. In Molecular cancer therapeutics, 23, 1652-1665. doi:10.1158/1535-7163.MCT-24-0145. https://pubmed.ncbi.nlm.nih.gov/38958503/
8. Jiang, Yi, Xu, Yuejie, Zhu, Chengliang, Lin, Hening, Zhang, Mingming. 2023. STAT3 palmitoylation initiates a positive feedback loop that promotes the malignancy of hepatocellular carcinoma cells in mice. In Science signaling, 16, eadd2282. doi:10.1126/scisignal.add2282. https://pubmed.ncbi.nlm.nih.gov/38051779/
9. Natale, Francesca, Spinelli, Matteo, Rinaudo, Marco, Fusco, Salvatore, Grassi, Claudio. 2024. Inhibition of zDHHC7-driven protein S-palmitoylation prevents cognitive deficits in an experimental model of Alzheimer's disease. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2402604121. doi:10.1073/pnas.2402604121. https://pubmed.ncbi.nlm.nih.gov/39589870/
10. Yang, Shu, Jia, Lijing, Xiang, Jiaqing, Liang, Zhen, Lu, Yan. 2022. KLF10 promotes nonalcoholic steatohepatitis progression through transcriptional activation of zDHHC7. In EMBO reports, 23, e54229. doi:10.15252/embr.202154229. https://pubmed.ncbi.nlm.nih.gov/35492028/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen