C57BL/6NCya-Tecrem1flox/Cya
Common Name:
Tecr-flox
Product ID:
S-CKO-00507
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Tecr-flox
Strain ID
CKOCMP-106529-Tecr-B6N-VA
Gene Name
Product ID
S-CKO-00507
Gene Alias
2410016D23Rik; A230102P12Rik; D17Ertd178e; Gpsn2; SC2
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tecrem1flox/Cya mice (Catalog S-CKO-00507) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019382
NCBI RefSeq
NM_134118
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Tecr, or trans-2-enoyl-CoA reductase, is an enzyme involved in very-long-chain fatty acid synthesis. It functions in the fourth step of the fatty acid elongation cycle and the sphingosine degradation pathway, playing a crucial role in lipid metabolism [3,4].
In cerebrovascular endothelial cells, EC-specific knockout of Tecr compromises angiogenesis and increases vascular permeability with high levels of transcytosis from neonatal to adult stages, while maintaining vascular tight junctions. Lipidomic analysis shows that Tecr expression is associated with omega-3 fatty acids, which suppress caveolae vesicles formation, suggesting its role in maintaining blood-brain barrier homeostasis [1]. Also, in some studies, homozygous TECR variants in individuals of Hutterite ancestry are associated with intellectual disability, expanding the phenotypic spectrum to include dolicocephaly and dysgenesis of the corpus callosum [2].
In conclusion, Tecr is essential for lipid metabolism-related biological processes. Studies using gene knockout models, especially in the context of blood-brain barrier function and certain genetic intellectual disability phenotypes, have revealed its significance in maintaining physiological functions and its potential as a therapeutic target in central nervous system diseases associated with blood-brain barrier dysfunction [1,2].
References:
1. Wang, Jinxuan, Xu, Jianxiong, Zang, Guangchao, Wang, Yeqi, Wang, Guixue. 2022. trans-2-Enoyl-CoA Reductase Tecr-Driven Lipid Metabolism in Endothelial Cells Protects against Transcytosis to Maintain Blood-Brain Barrier Homeostasis. In Research (Washington, D.C.), 2022, 9839368. doi:10.34133/2022/9839368. https://pubmed.ncbi.nlm.nih.gov/35465346/
2. Platt, Dylan, Davis-Keppen, Laura. . Individual with Homozygous TECR Variant Expands Upon the Existing Phenotype for a Hutterite Founder Mutation. In South Dakota medicine : the journal of the South Dakota State Medical Association, 75, 150-153. doi:. https://pubmed.ncbi.nlm.nih.gov/35709344/
3. Zhou, Youli, Lv, Rui, Ye, Richard D, Ren, Ruobing, Yu, Leiye. 2024. The 3-hydroxyacyl-CoA dehydratase 1/2 form complex with trans-2-enoyl-CoA reductase involved in substrates transfer in very long chain fatty acid elongation. In Biochemical and biophysical research communications, 704, 149588. doi:10.1016/j.bbrc.2024.149588. https://pubmed.ncbi.nlm.nih.gov/38422897/
4. Kato, Ryoya, Takenaka, Yuka, Ohno, Yusuke, Kihara, Akio. 2024. Catalytic mechanism of trans-2-enoyl-CoA reductases in the fatty acid elongation cycle and its cooperative action with fatty acid elongases. In The Journal of biological chemistry, 300, 105656. doi:10.1016/j.jbc.2024.105656. https://pubmed.ncbi.nlm.nih.gov/38224948/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen