C57BL/6JCya-Cds2em1flox/Cya
Common Name:
Cds2-flox
Product ID:
S-CKO-00908
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cds2-flox
Strain ID
CKOCMP-110911-Cds2-B6J-VA
Gene Name
Product ID
S-CKO-00908
Gene Alias
5730450N06Rik; 5730460C18Rik; D2Wsu127e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cds2em1flox/Cya mice (Catalog S-CKO-00908) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000103181
NCBI RefSeq
NM_138651
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Cds2, short for CDP-diacylglycerol synthetase-2, is a metabolic enzyme that controls phosphoinositide recycling. It converts phosphatidic acid (PA) to CDP-DG, an essential intermediate in the de novo synthesis of phosphatidylinositol (PI) [2,5]. This enzyme is involved in crucial biological processes such as vascular morphogenesis, lipid metabolism, and energy metabolism [1,2,4]. Genetic models, like gene knockout (KO) mouse models, have been instrumental in studying its functions.
In endothelial cells, genetic ablation of Cds2 in zebrafish and mice switches the output of VEGFA signaling from promoting angiogenesis to inducing vessel regression. This occurs due to reduced phosphatidylinositol (4,5)-bisphosphate (PIP2) availability, leading to phosphatidylinositol (3,4,5)-triphosphate (PIP3) deficiency and FOXO1 activation, ultimately suppressing tumor growth [1]. In primary mouse macrophages, Cds2 deficiency leads to increased de novo PA synthesis, while in sustained GPCR-stimulation of PLC, it fails to maintain enhanced PI synthesis via the 'PI cycle' [2]. In liver-specific Cds2-deficient mice, it causes hepatic steatosis, inflammation, and fibrosis due to impaired mitochondrial function and decreased mitochondrial PE levels [3].
In conclusion, Cds2 is essential for normal vascular development, lipid metabolism, and mitochondrial function. The use of KO mouse models has revealed its significance in diseases such as tumor growth regulation and the progression of non-alcoholic fatty liver disease (NAFLD) to non-alcoholic steatohepatitis (NASH) and fibrosis. Understanding Cds2 functions provides insights into the underlying mechanisms of these biological processes and diseases.
References:
1. Zhao, Wencao, Cao, Le, Ying, Hanru, Wu, Dianqing, Pan, Weijun. 2019. Endothelial CDS2 deficiency causes VEGFA-mediated vascular regression and tumor inhibition. In Cell research, 29, 895-910. doi:10.1038/s41422-019-0229-5. https://pubmed.ncbi.nlm.nih.gov/31501519/
2. Collins, Daniel M, Janardan, Vishnu, Barneda, David, Stephens, Len R, Hawkins, Phillip T. . CDS2 expression regulates de novo phosphatidic acid synthesis. In The Biochemical journal, 481, 1449-1473. doi:10.1042/BCJ20240456. https://pubmed.ncbi.nlm.nih.gov/39312194/
3. Xu, Jiesi, Chen, Siyu, Wang, Wei, Yang, Hongyuan, Huang, Xun. 2021. Hepatic CDP-diacylglycerol synthase 2 deficiency causes mitochondrial dysfunction and promotes rapid progression of NASH and fibrosis. In Science bulletin, 67, 299-314. doi:10.1016/j.scib.2021.10.014. https://pubmed.ncbi.nlm.nih.gov/36546079/
4. Zhang, Jingyu, Chen, Feifei, Wei, Wuhan, Jin, Peisheng, Li, Qiang. 2024. Nr-CWS regulates METTL3-mediated m6A modification of CDS2 mRNA in vascular endothelial cells and has prognostic significance. In Communications biology, 7, 1348. doi:10.1038/s42003-024-07047-y. https://pubmed.ncbi.nlm.nih.gov/39424634/
5. Blunsom, Nicholas J, Cockcroft, Shamshad. 2019. Phosphatidylinositol synthesis at the endoplasmic reticulum. In Biochimica et biophysica acta. Molecular and cell biology of lipids, 1865, 158471. doi:10.1016/j.bbalip.2019.05.015. https://pubmed.ncbi.nlm.nih.gov/31173893/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen