C57BL/6JCya-Clic1em1flox/Cya
Common Name
Clic1-flox
Product ID
S-CKO-00991
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-114584-Clic1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Clic1-flox Mouse (Catalog S-CKO-00991) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clic1-flox
Strain ID
CKOCMP-114584-Clic1-B6J-VA
Gene Name
Product ID
S-CKO-00991
Gene Alias
G6, Clcp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 17
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000007257
NCBI RefSeq
NM_033444
Target Region
Exon 2~4
Size of Effective Region
~1.2 kb
Overview of Gene Research
CLIC1, or Chloride intracellular channel 1, is a protein that exists as either a monomeric soluble protein or a non-covalent dimeric protein capable of forming an ion channel. It is involved in multiple biological processes. It promotes cell cycle progression, cancer stem cell (CSC) self-renewal, and plays roles in proliferation, cell volume regulation, tumour invasion, migration, and angiogenesis [1]. It is also associated with pathways like Wnt/β-catenin/TCF4 signaling, ROS/HIF1α signaling, and is involved in the regulation of redox state, calcium homeostasis, and the Nrf2/HO-1 pathway [1,2,6,7].
In glioblastoma, CLIC1 facilitates the G1/S phase transition and regulates glioma stem-like cells (GSCs), which are crucial for tumour resistance and recurrence. Inhibiting CLIC1 in glioma cells leads to apoptosis and reduced cell motility [1,4]. In pancreatic cancer, elevated CLIC1 expression, induced by matrix stiffness, promotes glycolytic metabolism and tumour proliferation [2]. In hepatocellular carcinoma, CLIC1 drives angiogenesis by modulating VEGFA, and its high expression is associated with poor prognosis [3]. In oral squamous cell carcinoma, higher CLIC1 plasma concentration is associated with lymph node metastases [5]. In esophageal cancer, KCTD4 binds to CLIC1, disrupts its dimerization, increases intracellular Ca2+ levels, and promotes metastasis [7]. In endothelial cells, inhibiting CLIC1 protects against cellular senescence and endothelial dysfunction via the Nrf2/HO-1 pathway [6]. In obesity models, Clic1 knockout mice ate less and had lower body weight, and pharmacological inhibition of Clic1 also reduced food intake and promoted weight loss [8].
In conclusion, CLIC1 is a multi-functional protein involved in various biological processes and diseases. Studies using gene knockout or knockdown models in different disease contexts have revealed its roles in cancer progression, angiogenesis, metastasis, endothelial function, and obesity. These findings suggest that CLIC1 could be a potential therapeutic target for treating these diseases.
References：
1. Randhawa, Kamaldeep, Jahani-Asl, Arezu. 2023. CLIC1 regulation of cancer stem cells in glioblastoma. In Current topics in membranes, 92, 99-123. doi:10.1016/bs.ctm.2023.09.004. https://pubmed.ncbi.nlm.nih.gov/38007271/
2. Zheng, Jia-Hao, Zhu, Yu-Heng, Yang, Jian, Sun, Yong-Wei, Liu, De-Jun. 2024. A CLIC1 network coordinates matrix stiffness and the Warburg effect to promote tumor growth in pancreatic cancer. In Cell reports, 43, 114633. doi:10.1016/j.celrep.2024.114633. https://pubmed.ncbi.nlm.nih.gov/39154343/
3. Wei, Xuyong, Pan, Binhua, Yang, Mengfan, Lin, Hanchao, Xu, Xiao. . CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA. In Technology in cancer research & treatment, 21, 15330338221106820. doi:10.1177/15330338221106820. https://pubmed.ncbi.nlm.nih.gov/35722791/
4. Wang, Chengcheng, He, Zheng. 2023. Multi-omics analysis reveals CLIC1 as a therapeutic vulnerability of gliomas. In Frontiers in pharmacology, 14, 1279370. doi:10.3389/fphar.2023.1279370. https://pubmed.ncbi.nlm.nih.gov/38027011/
5. Wojtera, Bartosz Paweł, Sobecka, Agnieszka, Szewczyk, Mateusz, Suchorska, Wiktoria Maria, Golusiński, Wojciech. . CLIC1 plasma concentration is associated with lymph node metastases in oral squamous cell carcinoma. In Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 32, 341-347. doi:10.17219/acem/154621. https://pubmed.ncbi.nlm.nih.gov/36251793/
6. Lu, Dezhao, Le, Yifei, Ding, Jiali, Mao, Wei, Zhu, Ji. 2021. CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway. In Cell biochemistry and biophysics, 79, 239-252. doi:10.1007/s12013-020-00959-6. https://pubmed.ncbi.nlm.nih.gov/33432550/
7. Zheng, Cancan, Yu, Xiaomei, Xu, Taoyang, Liu, Jinbao, Xu, Wen Wen. 2023. KCTD4 interacts with CLIC1 to disrupt calcium homeostasis and promote metastasis in esophageal cancer. In Acta pharmaceutica Sinica. B, 13, 4217-4233. doi:10.1016/j.apsb.2023.07.013. https://pubmed.ncbi.nlm.nih.gov/37799381/
8. Zapata, Rizaldy C, Zhang, Dinghong, Yoon, Dongmin, Petrascheck, Michael, Osborn, Olivia. 2023. Targeting Clic1 for the treatment of obesity: A novel therapeutic strategy to reduce food intake and body weight. In Molecular metabolism, 76, 101794. doi:10.1016/j.molmet.2023.101794. https://pubmed.ncbi.nlm.nih.gov/37604246/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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