C57BL/6NCya-Prdm15em1flox/Cya
Common Name:
Prdm15-flox
Product ID:
S-CKO-00995
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Prdm15-flox
Strain ID
CKOCMP-114604-Prdm15-B6N-VA
Gene Name
Product ID
S-CKO-00995
Gene Alias
C21orf83; E130018M06Rik; ORF62; Zfp298
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Prdm15em1flox/Cya mice (Catalog S-CKO-00995) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095849
NCBI RefSeq
NM_144789
Target Region
Exon 4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
PRDM15, a transcriptional regulator belonging to the PRDM family, has significant roles in multiple biological processes. It is involved in pathways like DNA damage repair, cell metabolism regulation, and is crucial for maintaining naive pluripotency, with its dysregulation often associated with various diseases [1,2,5]. Genetic models, such as knockout (KO) mouse models, are valuable tools for studying its functions.
In colorectal cancer, knockdown of PRDM15 inhibits DNA damage repair and increases radiosensitivity, as it promotes DNA repair by interacting with the DNA-PKcs and Ku70/Ku80 complex [1]. In B-cell lymphomas, abrogation of PRDM15 in KO models leads to a metabolic crisis and selective death of lymphoma cells, indicating its role in fueling the metabolic requirements of these cells [2]. In holoprosencephaly, genetic deletion of murine Prdm15 causes anterior/posterior (A/P) patterning defects and brain malformations, and in Xenopus laevis, morpholino-mediated knockdown of Prdm15 recapitulates anterior neural features seen in related syndromes [3,4].
In conclusion, PRDM15 is essential in regulating DNA damage repair, cell metabolism, and embryonic development. The use of KO mouse models and other loss-of-function experiments has revealed its significance in diseases like colorectal cancer, B-cell lymphomas, and syndromes related to holoprosencephaly, providing insights into potential therapeutic targets for these conditions.
References:
1. Yu, Yue, Liu, Tingting, Yu, Guanyu, Yang, Yanyong, Zhang, Wei. 2022. PRDM15 interacts with DNA-PK-Ku complex to promote radioresistance in rectal cancer by facilitating DNA damage repair. In Cell death & disease, 13, 978. doi:10.1038/s41419-022-05402-7. https://pubmed.ncbi.nlm.nih.gov/36402747/
2. Mzoughi, Slim, Fong, Jia Yi, Papadopoli, David, Topisirovic, Ivan, Guccione, Ernesto. 2020. PRDM15 is a key regulator of metabolism critical to sustain B-cell lymphomagenesis. In Nature communications, 11, 3520. doi:10.1038/s41467-020-17064-0. https://pubmed.ncbi.nlm.nih.gov/32665551/
3. Mzoughi, Slim, Di Tullio, Federico, Low, Diana H P, Messerschmidt, Daniel M, Guccione, Ernesto. 2020. PRDM15 loss of function links NOTCH and WNT/PCP signaling to patterning defects in holoprosencephaly. In Science advances, 6, eaax9852. doi:10.1126/sciadv.aax9852. https://pubmed.ncbi.nlm.nih.gov/31950080/
4. Saumweber, Ernestine, Mzoughi, Slim, Khadra, Arin, Schmeisser, Michael J, Kühl, Susanne J. 2024. Prdm15 acts upstream of Wnt4 signaling in anterior neural development of Xenopus laevis. In Frontiers in cell and developmental biology, 12, 1316048. doi:10.3389/fcell.2024.1316048. https://pubmed.ncbi.nlm.nih.gov/38444828/
5. Mzoughi, Slim, Zhang, Jingxian, Hequet, Delphine, Messerschmidt, Daniel M, Guccione, Ernesto. 2017. PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAPK-ERK signaling. In Nature genetics, 49, 1354-1363. doi:10.1038/ng.3922. https://pubmed.ncbi.nlm.nih.gov/28740264/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen