C57BL/6JCya-Hsd17b11em1flox/Cya
Common Name:
Hsd17b11-flox
Product ID:
S-CKO-01007
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Hsd17b11-flox
Strain ID
CKOCMP-114664-Hsd17b11-B6J-VA
Gene Name
Product ID
S-CKO-01007
Gene Alias
Dhrs8; Pan1b; SDR2; retSDR2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsd17b11em1flox/Cya mice (Catalog S-CKO-01007) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031251
NCBI RefSeq
NM_053262
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Hsd17b11, encoding type 11 17β-hydroxysteroid dehydrogenase, catalyzes the conversion of 5α-androstan-3α,17β-diol into androsterone, suggesting its role in androgen metabolism [7]. It is also associated with fatty acid and arachidonic acid absorption and metabolism [2].
In cancer research, its high expression in CRC primary tissues was related to poor prognosis, and it promoted CRC cell proliferation and metastasis through the lnc-HSD17B11-1:1/miR-338-3p/MACC1 axis [1]. In LUAD, low expression of Hsd17b11 was associated with increased recurrence rate [2]. In pancreatic cancer, its differential expression was found, and it was significantly associated with overall survival [5]. In esophageal cancer, FTO promoted lipid droplet formation in cells by enhancing Hsd17b11 expression [6]. Also, Hsd17B11 was identified as a mediator of the selective cytotoxic effects of dehydrofalcarinol in MSL subtype triple-negative breast cancer cells [8]. In addition, phenyl-dialkynylcarbinols can be bio-oxidized by HSD17B11, leading to endoplasmic reticulum stress and apoptosis [3]. Moreover, in hepatocytes, HSD17B11 was found to be an interaction partner contributing to GCKIII-mediated regulation of liver lipid homeostasis [4].
In conclusion, Hsd17b11 is involved in androgen and lipid metabolism. Its dysregulation is associated with various cancers, affecting tumor progression, metastasis, and patient prognosis. Functional studies, especially in the context of cancer, contribute to understanding its role in disease mechanisms, potentially providing new diagnostic and therapeutic targets.
References:
1. Zhang, Wei, Wang, Bo, Wang, Quan, Jiang, Kewei, Wang, Shan. 2020. Lnc-HSD17B11-1:1 Functions as a Competing Endogenous RNA to Promote Colorectal Cancer Progression by Sponging miR-338-3p to Upregulate MACC1. In Frontiers in genetics, 11, 628. doi:10.3389/fgene.2020.00628. https://pubmed.ncbi.nlm.nih.gov/32595704/
2. Wei, Chunhui, Li, Lixia, Qiao, Youping, Huang, Dan, Wu, Dong. . Ferroptosis-related genes DUOX1 and HSD17B11 affect tumor microenvironment and predict overall survival of lung adenocarcinoma patients. In Medicine, 103, e38322. doi:10.1097/MD.0000000000038322. https://pubmed.ncbi.nlm.nih.gov/39259123/
3. Bossuat, Margaux, Rullière, Pauline, Preuilh, Nadège, Britton, Sébastien, Génisson, Yves. 2023. Phenyl dialkynylcarbinols, a Bioinspired Series of Synthetic Antitumor Acetylenic Lipids. In Journal of medicinal chemistry, 66, 13918-13945. doi:10.1021/acs.jmedchem.3c00859. https://pubmed.ncbi.nlm.nih.gov/37816126/
4. Cansby, Emmelie, Caputo, Mara, Andersson, Emma, Hoy, Andrew J, Mahlapuu, Margit. 2024. GCKIII kinases control hepatocellular lipid homeostasis via shared mode of action. In Journal of lipid research, 65, 100669. doi:10.1016/j.jlr.2024.100669. https://pubmed.ncbi.nlm.nih.gov/39395791/
5. Bai, Rubing, Rebelo, Artur, Kleeff, Jörg, Sunami, Yoshiaki. 2021. Identification of prognostic lipid droplet-associated genes in pancreatic cancer patients via bioinformatics analysis. In Lipids in health and disease, 20, 58. doi:10.1186/s12944-021-01476-y. https://pubmed.ncbi.nlm.nih.gov/34078402/
6. Duan, Xiaoran, Yang, Li, Wang, Liuya, Qin, Guohui, Zhang, Yi. 2022. m6A demethylase FTO promotes tumor progression via regulation of lipid metabolism in esophageal cancer. In Cell & bioscience, 12, 60. doi:10.1186/s13578-022-00798-3. https://pubmed.ncbi.nlm.nih.gov/35568876/
7. Rotinen, Mirja, Villar, Joaquín, Celay, Jon, Notario, Vicente, Encío, Ignacio. 2011. Transcriptional regulation of type 11 17β-hydroxysteroid dehydrogenase expression in prostate cancer cells. In Molecular and cellular endocrinology, 339, 45-53. doi:10.1016/j.mce.2011.03.015. https://pubmed.ncbi.nlm.nih.gov/21549806/
8. Grant, Corena V, Cai, Shengxin, Risinger, April L, Cichewicz, Robert H, Mooberry, Susan L. 2020. CRISPR-Cas9 Genome-Wide Knockout Screen Identifies Mechanism of Selective Activity of Dehydrofalcarinol in Mesenchymal Stem-like Triple-Negative Breast Cancer Cells. In Journal of natural products, 83, 3080-3092. doi:10.1021/acs.jnatprod.0c00642. https://pubmed.ncbi.nlm.nih.gov/33021790/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen