C57BL/6JCya-Alox12em1flox/Cya
Common Name
Alox12-flox
Product ID
S-CKO-01150
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-11684-Alox12-B6J-VA
Status
When using this mouse strain in a publication, please cite “Alox12-flox Mouse (Catalog S-CKO-01150) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Alox12-flox
Strain ID
CKOCMP-11684-Alox12-B6J-VA
Gene Name
Product ID
S-CKO-01150
Gene Alias
P-12LO, Alox12p, 9930022G08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 11
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000000329
NCBI RefSeq
NM_007440
Target Region
Exon 3~6
Size of Effective Region
~1.7 kb
Overview of Gene Research
Alox12, also known as arachidonate 12-lipoxygenase, acts on polyunsaturated fatty acid substrates to produce bioactive lipid mediators like eicosanes and lipoxins. It plays a crucial role in inflammation and oxidation processes, and is involved in multiple biological pathways related to various diseases [9].
In p53-mediated tumour suppression, Alox12 inactivation diminishes p53-mediated ferroptosis and abrogates p53-dependent inhibition of tumour growth in xenograft models, suggesting it is critical for this process [1]. In hepatic ischemia-reperfusion injury, the ALOX12-12-HETE-GPR31 signaling axis is a key determinant, and blocking 12-HETE production (regulated by Alox12) can attenuate liver damage [2]. In non-alcoholic steatohepatitis (NASH), a small molecule IMA-1 that interrupts the Alox12-ACC1 interaction can treat NASH in mice and macaques [3]. Gα12 overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated dysregulation of Alox12 [4]. Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting Alox12-dependent ferroptosis [5]. In hepatic ischemia-reperfusion injury, TRPM2-mediated calcium influx increases Alox12 expression, leading to mitochondrial lipid peroxidation [6]. In lung ischemia-reperfusion injury, Alox12 and its metabolite 12-HETE promote ferroptosis and neutrophil extracellular trap formation, and Alox12-knockout mice show decreased injury [7]. In myocardial ischemia-reperfusion injury, genetic inhibition of Alox12 protects mouse hearts, while pharmacological inhibition reduces cardiac injury in multiple species [8]. In diabetic kidney disease, Alox12 levels in renal tissue are elevated and can predict disease progression [10].
In conclusion, Alox12 is involved in multiple biological processes, especially those related to ferroptosis, inflammation, and lipid peroxidation. Gene-knockout mouse models have been instrumental in revealing its role in various disease conditions such as cancer, ischemia-reperfusion injuries, and diabetic kidney disease, providing potential therapeutic targets for these diseases.
References：
1. Chu, Bo, Kon, Ning, Chen, Delin, Tavana, Omid, Gu, Wei. 2019. ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway. In Nature cell biology, 21, 579-591. doi:10.1038/s41556-019-0305-6. https://pubmed.ncbi.nlm.nih.gov/30962574/
2. Zhang, Xiao-Jing, Cheng, Xu, Yan, Zhen-Zhen, She, Zhi-Gang, Li, Hongliang. 2017. An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. In Nature medicine, 24, 73-83. doi:10.1038/nm.4451. https://pubmed.ncbi.nlm.nih.gov/29227475/
3. Zhang, Xiao-Jing, Ji, Yan-Xiao, Cheng, Xu, She, Zhi-Gang, Li, Hongliang. 2021. A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques. In Science translational medicine, 13, eabg8116. doi:10.1126/scitranslmed.abg8116. https://pubmed.ncbi.nlm.nih.gov/34910548/
4. Tak, Jihoon, Kim, Yun Seok, Kim, Tae Hyun, Hwang, Shin, Kim, Sang Geon. 2022. Gα12 overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated miR-15a and ALOX12 dysregulation. In Theranostics, 12, 1570-1588. doi:10.7150/thno.67722. https://pubmed.ncbi.nlm.nih.gov/35198058/
5. Guo, Shanshan, Zhou, Lang, Liu, Xueqi, Li, Yuanyuan, Wu, Yonggui. 2024. Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting ALOX12-dependent ferroptosis. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 130, 155757. doi:10.1016/j.phymed.2024.155757. https://pubmed.ncbi.nlm.nih.gov/38805781/
6. Zhong, Cheng, Yang, Jing, Zhang, Yiyin, Yu, Peilin, Lin, Hui. 2023. TRPM2 Mediates Hepatic Ischemia-Reperfusion Injury via Ca2+-Induced Mitochondrial Lipid Peroxidation through Increasing ALOX12 Expression. In Research (Washington, D.C.), 6, 0159. doi:10.34133/research.0159. https://pubmed.ncbi.nlm.nih.gov/37275121/
7. Li, Chongwu, Gao, Peigen, Zhuang, Fenghui, Wu, Junqi, Chen, Chang. 2024. Inhibition of ALOX12-12-HETE Alleviates Lung Ischemia-Reperfusion Injury by Reducing Endothelial Ferroptosis-Mediated Neutrophil Extracellular Trap Formation. In Research (Washington, D.C.), 7, 0473. doi:10.34133/research.0473. https://pubmed.ncbi.nlm.nih.gov/39268501/
8. Zhang, Xiao-Jing, Liu, Xiaolan, Hu, Manli, Lu, Zhibing, Li, Hongliang. 2021. Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species. In Cell metabolism, 33, 2059-2075.e10. doi:10.1016/j.cmet.2021.08.014. https://pubmed.ncbi.nlm.nih.gov/34536344/
9. Zheng, Zhonghua, Li, Yin, Jin, Gehui, Zou, Mengsha, Duan, Shiwei. 2020. The biological role of arachidonic acid 12-lipoxygenase (ALOX12) in various human diseases. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 129, 110354. doi:10.1016/j.biopha.2020.110354. https://pubmed.ncbi.nlm.nih.gov/32540644/
10. Wang, Meixi, Wang, Jingjing, Wang, Jinni, Wu, Yonggui, Qi, Xiangming. 2024. Elevated ALOX12 in renal tissue predicts progression in diabetic kidney disease. In Renal failure, 46, 2313182. doi:10.1080/0886022X.2024.2313182. https://pubmed.ncbi.nlm.nih.gov/38345057/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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