C57BL/6JCya-Alox12em1flox/Cya
Common Name:
Alox12-flox
Product ID:
S-CKO-01150
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Alox12-flox
Strain ID
CKOCMP-11684-Alox12-B6J-VA
Gene Name
Product ID
S-CKO-01150
Gene Alias
9930022G08Rik; Alox12p; P-12LO
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Alox12em1flox/Cya mice (Catalog S-CKO-01150) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000000329
NCBI RefSeq
NM_007440
Target Region
Exon 3~6
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Alox12, also known as arachidonate 12-lipoxygenase, acts on polyunsaturated fatty acid substrates to produce bioactive lipid mediators like eicosanes and lipoxins. It plays a crucial role in inflammation and oxidation processes, and is involved in multiple biological pathways related to various diseases [9].
In p53-mediated tumour suppression, Alox12 inactivation diminishes p53-mediated ferroptosis and abrogates p53-dependent inhibition of tumour growth in xenograft models, suggesting it is critical for this process [1]. In hepatic ischemia-reperfusion injury, the ALOX12-12-HETE-GPR31 signaling axis is a key determinant, and blocking 12-HETE production (regulated by Alox12) can attenuate liver damage [2]. In non-alcoholic steatohepatitis (NASH), a small molecule IMA-1 that interrupts the Alox12-ACC1 interaction can treat NASH in mice and macaques [3]. Gα12 overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated dysregulation of Alox12 [4]. Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting Alox12-dependent ferroptosis [5]. In hepatic ischemia-reperfusion injury, TRPM2-mediated calcium influx increases Alox12 expression, leading to mitochondrial lipid peroxidation [6]. In lung ischemia-reperfusion injury, Alox12 and its metabolite 12-HETE promote ferroptosis and neutrophil extracellular trap formation, and Alox12-knockout mice show decreased injury [7]. In myocardial ischemia-reperfusion injury, genetic inhibition of Alox12 protects mouse hearts, while pharmacological inhibition reduces cardiac injury in multiple species [8]. In diabetic kidney disease, Alox12 levels in renal tissue are elevated and can predict disease progression [10].
In conclusion, Alox12 is involved in multiple biological processes, especially those related to ferroptosis, inflammation, and lipid peroxidation. Gene-knockout mouse models have been instrumental in revealing its role in various disease conditions such as cancer, ischemia-reperfusion injuries, and diabetic kidney disease, providing potential therapeutic targets for these diseases.
References:
1. Chu, Bo, Kon, Ning, Chen, Delin, Tavana, Omid, Gu, Wei. 2019. ALOX12 is required for p53-mediated tumour suppression through a distinct ferroptosis pathway. In Nature cell biology, 21, 579-591. doi:10.1038/s41556-019-0305-6. https://pubmed.ncbi.nlm.nih.gov/30962574/
2. Zhang, Xiao-Jing, Cheng, Xu, Yan, Zhen-Zhen, She, Zhi-Gang, Li, Hongliang. 2017. An ALOX12-12-HETE-GPR31 signaling axis is a key mediator of hepatic ischemia-reperfusion injury. In Nature medicine, 24, 73-83. doi:10.1038/nm.4451. https://pubmed.ncbi.nlm.nih.gov/29227475/
3. Zhang, Xiao-Jing, Ji, Yan-Xiao, Cheng, Xu, She, Zhi-Gang, Li, Hongliang. 2021. A small molecule targeting ALOX12-ACC1 ameliorates nonalcoholic steatohepatitis in mice and macaques. In Science translational medicine, 13, eabg8116. doi:10.1126/scitranslmed.abg8116. https://pubmed.ncbi.nlm.nih.gov/34910548/
4. Tak, Jihoon, Kim, Yun Seok, Kim, Tae Hyun, Hwang, Shin, Kim, Sang Geon. 2022. Gα12 overexpression in hepatocytes by ER stress exacerbates acute liver injury via ROCK1-mediated miR-15a and ALOX12 dysregulation. In Theranostics, 12, 1570-1588. doi:10.7150/thno.67722. https://pubmed.ncbi.nlm.nih.gov/35198058/
5. Guo, Shanshan, Zhou, Lang, Liu, Xueqi, Li, Yuanyuan, Wu, Yonggui. 2024. Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting ALOX12-dependent ferroptosis. In Phytomedicine : international journal of phytotherapy and phytopharmacology, 130, 155757. doi:10.1016/j.phymed.2024.155757. https://pubmed.ncbi.nlm.nih.gov/38805781/
6. Zhong, Cheng, Yang, Jing, Zhang, Yiyin, Yu, Peilin, Lin, Hui. 2023. TRPM2 Mediates Hepatic Ischemia-Reperfusion Injury via Ca2+-Induced Mitochondrial Lipid Peroxidation through Increasing ALOX12 Expression. In Research (Washington, D.C.), 6, 0159. doi:10.34133/research.0159. https://pubmed.ncbi.nlm.nih.gov/37275121/
7. Li, Chongwu, Gao, Peigen, Zhuang, Fenghui, Wu, Junqi, Chen, Chang. 2024. Inhibition of ALOX12-12-HETE Alleviates Lung Ischemia-Reperfusion Injury by Reducing Endothelial Ferroptosis-Mediated Neutrophil Extracellular Trap Formation. In Research (Washington, D.C.), 7, 0473. doi:10.34133/research.0473. https://pubmed.ncbi.nlm.nih.gov/39268501/
8. Zhang, Xiao-Jing, Liu, Xiaolan, Hu, Manli, Lu, Zhibing, Li, Hongliang. 2021. Pharmacological inhibition of arachidonate 12-lipoxygenase ameliorates myocardial ischemia-reperfusion injury in multiple species. In Cell metabolism, 33, 2059-2075.e10. doi:10.1016/j.cmet.2021.08.014. https://pubmed.ncbi.nlm.nih.gov/34536344/
9. Zheng, Zhonghua, Li, Yin, Jin, Gehui, Zou, Mengsha, Duan, Shiwei. 2020. The biological role of arachidonic acid 12-lipoxygenase (ALOX12) in various human diseases. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 129, 110354. doi:10.1016/j.biopha.2020.110354. https://pubmed.ncbi.nlm.nih.gov/32540644/
10. Wang, Meixi, Wang, Jingjing, Wang, Jinni, Wu, Yonggui, Qi, Xiangming. 2024. Elevated ALOX12 in renal tissue predicts progression in diabetic kidney disease. In Renal failure, 46, 2313182. doi:10.1080/0886022X.2024.2313182. https://pubmed.ncbi.nlm.nih.gov/38345057/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen