C57BL/6JCya-Noctem1flox/Cya
Common Name:
Noct-flox
Product ID:
S-CKO-01597
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Noct-flox
Strain ID
CKOCMP-12457-Noct-B6J-VA
Gene Name
Product ID
S-CKO-01597
Gene Alias
Ccr4; Ccrn4l
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Noctem1flox/Cya mice (Catalog S-CKO-01597) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023849
NCBI RefSeq
NM_009834
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Nocturnin (Noct), also known as Ccrn4l, is a protein encoded by the Noct gene. It belongs to the EEP family of proteins, with close similarity to the CCR4 family of deadenylases. Noct is an output of the circadian clock, and its mRNA is broadly expressed in cells throughout the body, with a particularly high-amplitude rhythm in the liver. It is involved in multiple biological processes such as development, adipogenesis, lipid metabolism, inflammation, osteogenesis, and obesity, and is also associated with the regulation of NAD(H) and NADP(H) homeostasis [1,2].
Mice lacking Noct (Noct KO or Noct-/-) are protected from high-fat diet-induced obesity and hepatic steatosis, indicating that Noct plays a role in lipid metabolism and obesity-related processes [1]. A mouse model overexpressing Noct showed decreased fat mass in male mice, likely through its role as a mediator of mitochondrial function [3].
In conclusion, Noct is a key gene regulated by the circadian clock. Model-based research, especially the use of Noct KO mouse models, has revealed its important functions in lipid metabolism, adipogenesis, and obesity-related diseases. Understanding Noct's functions provides insights into the molecular mechanisms underlying these biological processes and diseases.
References:
1. Kulshrestha, Shruti, Devkar, Ranjitsinh. 2023. Circadian control of Nocturnin and its regulatory role in health and disease. In Chronobiology international, 40, 970-981. doi:10.1080/07420528.2023.2231081. https://pubmed.ncbi.nlm.nih.gov/37400970/
2. Chen, Luojun, Xing, Xiaoke, Zhang, Pingfeng, Chen, Lulu, Pei, Huadong. 2023. Homeostatic regulation of NAD(H) and NADP(H) in cells. In Genes & diseases, 11, 101146. doi:10.1016/j.gendis.2023.101146. https://pubmed.ncbi.nlm.nih.gov/38988322/
3. Le, Phuong T, Bornstein, Sheila A, Motyl, Katherine J, Rosen, Clifford J, Guntur, Anyonya R. 2019. A novel mouse model overexpressing Nocturnin results in decreased fat mass in male mice. In Journal of cellular physiology, 234, 20228-20239. doi:10.1002/jcp.28623. https://pubmed.ncbi.nlm.nih.gov/30953371/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen