C57BL/6JCya-Entpd1em1flox/Cya
Common Name
Entpd1-flox
Product ID
S-CKO-01623
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12495-Entpd1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Entpd1-flox Mouse (Catalog S-CKO-01623) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Entpd1-flox
Strain ID
CKOCMP-12495-Entpd1-B6J-VA
Gene Name
Product ID
S-CKO-01623
Gene Alias
Cd39, NTPDase-1, 2610206B08Rik, E130009M23Rik
Background
C57BL/6JCya
Gene Full Name
ectonucleoside triphosphate diphosphohydrolase 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 19 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000112231
NCBI Transcript ID
NM_009848
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Overview of Gene Research
Entpd1, also known as CD39, is an ectonucleotidase that plays a crucial role in the conversion of ADP/ATP to AMP as part of the CD39-CD73-adenosine pathway [1,2,3]. This pathway is important in calibrating purinergic signals to immune cells, shifting the environment from pro-inflammatory (ATP-driven) to anti-inflammatory (adenosine-induced) [2].
In cancer, Entpd1 expressed on regulatory T cells (Tregs) inhibits effector lymphocytes, creating a favorable environment for tumor growth. Knockout models and surrogate inhibitors of CD39 support the anticancer activity of CD39 inhibition, predicting a good safety profile for inhibitory compounds [1]. In hepatic metastatic cancer mouse models, Cd39 null vasculature or Cd39 null bone marrow-derived cells strongly inhibited tumor growth, indicating that CD39 expression on Tregs promotes metastatic growth [5]. In septic CD39 -/- mice, there were higher levels of inflammatory cytokines and more pronounced liver injury compared to wild-type mice, suggesting CD39 attenuates sepsis-associated liver injury [4].
In conclusion, Entpd1 is essential in regulating the immune environment through the CD39-CD73-adenosine pathway. Gene knockout mouse models have been instrumental in revealing its role in cancer and sepsis-related liver injury, highlighting its potential as a therapeutic target in oncology and for managing sepsis-induced hepatic damage.
References：
1. Bastid, J, Cottalorda-Regairaz, A, Alberici, G, Eliaou, J-F, Bensussan, A. 2012. ENTPD1/CD39 is a promising therapeutic target in oncology. In Oncogene, 32, 1743-51. doi:10.1038/onc.2012.269. https://pubmed.ncbi.nlm.nih.gov/22751118/
2. Antonioli, Luca, Pacher, Pál, Vizi, E Sylvester, Haskó, György. 2013. CD39 and CD73 in immunity and inflammation. In Trends in molecular medicine, 19, 355-67. doi:10.1016/j.molmed.2013.03.005. https://pubmed.ncbi.nlm.nih.gov/23601906/
3. Timperi, Eleonora, Barnaba, Vincenzo. 2021. CD39 Regulation and Functions in T Cells. In International journal of molecular sciences, 22, . doi:10.3390/ijms22158068. https://pubmed.ncbi.nlm.nih.gov/34360833/
4. Savio, Luiz Eduardo Baggio, de Andrade Mello, Paola, Figliuolo, Vanessa R, Robson, Simon C, Coutinho-Silva, Robson. 2017. CD39 limits P2X7 receptor inflammatory signaling and attenuates sepsis-induced liver injury. In Journal of hepatology, 67, 716-726. doi:10.1016/j.jhep.2017.05.021. https://pubmed.ncbi.nlm.nih.gov/28554875/
5. Sun, Xiaofeng, Wu, Yan, Gao, Wenda, Murakami, Takashi, Robson, Simon C. 2010. CD39/ENTPD1 expression by CD4+Foxp3+ regulatory T cells promotes hepatic metastatic tumor growth in mice. In Gastroenterology, 139, 1030-40. doi:10.1053/j.gastro.2010.05.007. https://pubmed.ncbi.nlm.nih.gov/20546740/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
