C57BL/6JCya-Cluem1flox/Cya
Common Name
Clu-flox
Product ID
S-CKO-01762
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-12759-Clu-B6J-VA
When using this mouse strain in a publication, please cite “Clu-flox Mouse (Catalog S-CKO-01762) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Clu-flox
Strain ID
CKOCMP-12759-Clu-B6J-VA
Gene Name
Product ID
S-CKO-01762
Gene Alias
Cli, ApoJ, Sgp2, SP-40, Sgp-2, Sugp-2, D14Ucla3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 14
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000022616
NCBI RefSeq
NM_013492
Target Region
Exon 4~5
Size of Effective Region
~1.9 kb
Overview of Gene Research
CLU, also known as clusterin or apolipoprotein J (apoJ), is a highly evolutionary conserved glycoprotein. It functions as a molecular chaperone and is involved in a broad range of physiological and pathophysiological functions, exerting a cytoprotective role. It participates in processes like programmed cell death, metastasis, invasion, proliferation, and cell growth, regulating diverse signaling pathways [2,3].
In oral cancer cells, CLU localizes to mitochondria and acts as an adaptor protein. It coordinates with BAX and LC3 to induce mitophagy in response to cisplatin treatment, controlling mitochondrial damage. Inhibition of its related mitophagic flux can lead to ROS-dependent apoptosis. Also, in oral cancer stem cells, CLU promotes mitophagic degradation of MSX2 through an AKT-DNM1L/Drp1 axis, maintaining SOX2-mediated stemness. Knockdown of CLU disturbs mitochondrial metabolism and improves cisplatin sensitivity [1,4].
In conclusion, CLU plays essential roles in maintaining mitochondrial homeostasis, cell survival, and cancer cell stemness. Studies, especially those related to its knockdown in oral cancer models, reveal its significance in cancer biology, suggesting that targeting CLU could potentially be a therapeutic strategy against oral cancer.
References：
1. Praharaj, Prakash P, Patra, Srimanta, Singh, Amruta, Chae, Han J, Bhutia, Sujit K. 2024. CLU (clusterin) and PPARGC1A/PGC1α coordinately control mitophagy and mitochondrial biogenesis for oral cancer cell survival. In Autophagy, 20, 1359-1382. doi:10.1080/15548627.2024.2309904. https://pubmed.ncbi.nlm.nih.gov/38447939/
2. Rohne, Philipp, Prochnow, Hans, Koch-Brandt, Claudia. . The CLU-files: disentanglement of a mystery. In Biomolecular concepts, 7, 1-15. doi:10.1515/bmc-2015-0026. https://pubmed.ncbi.nlm.nih.gov/26673020/
3. Zhang, Yefei, Lv, Xiang, Chen, Liming, Liu, Yan. 2022. The role and function of CLU in cancer biology and therapy. In Clinical and experimental medicine, 23, 1375-1391. doi:10.1007/s10238-022-00885-2. https://pubmed.ncbi.nlm.nih.gov/36098834/
4. Praharaj, Prakash P, Patra, Srimanta, Mishra, Soumya R, Patil, Shankargouda, Bhutia, Sujit K. 2023. CLU (clusterin) promotes mitophagic degradation of MSX2 through an AKT-DNM1L/Drp1 axis to maintain SOX2-mediated stemness in oral cancer stem cells. In Autophagy, 19, 2196-2216. doi:10.1080/15548627.2023.2178876. https://pubmed.ncbi.nlm.nih.gov/36779631/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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