C57BL/6NCya-Cnr2em1flox/Cya
Common Name:
Cnr2-flox
Product ID:
S-CKO-01791
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cnr2-flox
Strain ID
CKOCMP-12802-Cnr2-B6N-VA
Gene Name
Product ID
S-CKO-01791
Gene Alias
CB-2; CB2; CB2-R
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Cnr2em1flox/Cya mice (Catalog S-CKO-01791) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000097843
NCBI RefSeq
NM_009924
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Cnr2, encoding cannabinoid receptor type 2 (CB2R), is mainly expressed by leukocytes and has a role in the immune system. It can modulate immune responses and is involved in various biological processes such as implantation, sensory information processing, and the regulation of anxiety and stress recovery. It is part of the endogenous cannabinoid system (ECS), which plays a role in many physiological functions [2,3,5].
Using a knock-in mouse model expressing a FLAG-tagged Cnr2 gene, researchers found that cannabinoids impair the function of tumor-specific T cells through Cnr2. Cnr2 binds to JAK1 and inhibits the downstream STAT signaling in T cells, suppressing antitumor immunity [1].
Cnr2-deficient mice show resistance to lipopolysaccharide (LPS)-driven preterm birth due to increased IL-10 production via augmented cAMP accumulation [4]. In addition, Cnr2-deficient mice have compromised implantation, with uterine epithelium abnormalities and blood vessel leakage during implantation [3].
In conclusion, Cnr2 is crucial for multiple biological functions. Its role in suppressing antitumor immunity through the JAK/STAT signaling pathway in T cells, as well as its functions in implantation and inflammation-induced preterm birth, are revealed by gene-knockout mouse models. Understanding Cnr2 functions helps to better comprehend related biological processes and may provide insights for treating relevant diseases [1,3,4].
References：
1. Xiong, Xinxin, Chen, Siyu, Shen, Jianfei, Li, Wende, Zhou, Penghui. 2022. Cannabis suppresses antitumor immunity by inhibiting JAK/STAT signaling in T cells through CNR2. In Signal transduction and targeted therapy, 7, 99. doi:10.1038/s41392-022-00918-y. https://pubmed.ncbi.nlm.nih.gov/35383142/
2. Grabon, Wanda, Bodennec, Jacques, Rheims, Sylvain, Belmeguenai, Amor, Bezin, Laurent. 2023. Update on the controversial identity of cells expressing cnr2 gene in the nervous system. In CNS neuroscience & therapeutics, 29, 760-770. doi:10.1111/cns.13977. https://pubmed.ncbi.nlm.nih.gov/36604187/
3. Li, Yingju, Bian, Fenghua, Sun, Xiaofei, Dey, Sudhansu K. . Mice Missing Cnr1 and Cnr2 Show Implantation Defects. In Endocrinology, 160, 938-946. doi:10.1210/en.2019-00024. https://pubmed.ncbi.nlm.nih.gov/30776303/
4. Sun, Xiaofei, Cappelletti, Monica, Li, Yingju, Divanovic, Senad, Dey, Sudhansu K. 2014. Cnr2 deficiency confers resistance to inflammation-induced preterm birth in mice. In Endocrinology, 155, 4006-14. doi:10.1210/en.2014-1387. https://pubmed.ncbi.nlm.nih.gov/25051450/
5. Peiró, Ana M, García-Gutiérrez, María S, Planelles, Beatriz, Bobes, Julio, Manzanares, Jorge. 2020. Association of cannabinoid receptor genes (CNR1 and CNR2) polymorphisms and panic disorder. In Anxiety, stress, and coping, 33, 256-265. doi:10.1080/10615806.2020.1732358. https://pubmed.ncbi.nlm.nih.gov/32114795/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen