C57BL/6JCya-Cry1em1flox/Cya
Common Name:
Cry1-flox
Product ID:
S-CKO-01881
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Cry1-flox
Strain ID
CKOCMP-12952-Cry1-B6J-VA
Gene Name
Product ID
S-CKO-01881
Gene Alias
Phll1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cry1em1flox/Cya mice (Catalog S-CKO-01881) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020227
NCBI RefSeq
NM_007771
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Cry1, short for Cryptochrome1, is a core component of the mammalian circadian clock, playing a crucial role in regulating ∼24-hour rhythms in various behavioral and physiological processes [1,4]. It is involved in the negative feedback loop of the circadian clock mechanism along with genes like Per1, Per2, and Cry2. Additionally, it has implications in processes such as drug metabolism regulation in the liver and glucose homeostasis maintenance [2,3].
In mouse models, ablation of Cry1 in mice reduced hepatic CYP2A5 expression, blunted its diurnal rhythms, decreased CYP2A5 activity, and exacerbated coumarin-induced hepatotoxicity, indicating that Cry1 positively regulates CYP2A5 expression and rhythms through repression of E4BP4 [2]. In diabetic mice, a reduction in hepatic Cry1 protein, stimulated by elevated E3 ligase FBXL3-dependent proteasomal degradation, contributed to hyperglycemia. GSK3β-induced Cry1 phosphorylation further potentiated this degradation, and GSK3β inhibitors decreased hepatic glucose production by facilitating Cry1-mediated FOXO1 degradation [3].
In conclusion, Cry1 is essential for maintaining normal circadian period, regulating drug metabolism in the liver, and maintaining glucose homeostasis. Studies using gene knockout mouse models have provided valuable insights into the role of Cry1 in diabetes-related hyperglycemia and the regulation of drug-metabolizing enzymes, helping us understand the underlying biological mechanisms and potentially providing new strategies for treating related diseases.
References:
1. Patke, Alina, Murphy, Patricia J, Onat, Onur Emre, Campbell, Scott S, Young, Michael W. . Mutation of the Human Circadian Clock Gene CRY1 in Familial Delayed Sleep Phase Disorder. In Cell, 169, 203-215.e13. doi:10.1016/j.cell.2017.03.027. https://pubmed.ncbi.nlm.nih.gov/28388406/
2. Lin, Luomin, Huang, Yuwei, Wang, Jinyi, Guo, Lianxia, Wu, Baojian. 2023. CRY1/2 regulate rhythmic CYP2A5 in mouse liver through repression of E4BP4. In Biochemical pharmacology, 217, 115843. doi:10.1016/j.bcp.2023.115843. https://pubmed.ncbi.nlm.nih.gov/37797722/
3. Kim, Ye Young, Jang, Hagoon, Lee, Gung, Kim, Jong-Seo, Kim, Jae Bum. . Hepatic GSK3β-Dependent CRY1 Degradation Contributes to Diabetic Hyperglycemia. In Diabetes, 71, 1373-1387. doi:10.2337/db21-0649. https://pubmed.ncbi.nlm.nih.gov/35476750/
4. Schirmer, Aaron E, Kumar, Vivek, Schook, Andrew, Marshall, Michael S, Takahashi, Joseph S. 2023. Cry1 expression during postnatal development is critical for the establishment of normal circadian period. In Frontiers in neuroscience, 17, 1166137. doi:10.3389/fnins.2023.1166137. https://pubmed.ncbi.nlm.nih.gov/37389366/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen