C57BL/6JCya-Ctskem1flox/Cya
Common Name:
Ctsk-flox
Product ID:
S-CKO-01940
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ctsk-flox
Strain ID
CKOCMP-13038-Ctsk-B6J-VA
Gene Name
Product ID
S-CKO-01940
Gene Alias
MMS10-Q; Ms10q; catK
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ctskem1flox/Cya mice (Catalog S-CKO-01940) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015664
NCBI RefSeq
NM_007802
Target Region
Exon 2~4
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Ctsk, encoding cathepsin K, is a cysteine protease. It has a strong activity in degrading extracellular matrix and is closely related to osteoclast-mediated bone destruction [1,3,4,5,6,7,8,9]. It may be involved in the IL-17 signaling pathway, as indicated by pathway enrichment analysis in castration-resistant prostate cancer (CRPC) research [1].
In a mouse model (Ctsk-Cre;Trp53f/f/Rb1f/f), ablation of Trp53/Rb1 in Ctsk-expressing cells led to spontaneous development of osteosarcoma, and inhibition of YAP/TAZ by verteporfin (VP) delayed osteosarcoma progression [2]. In the study of postmenopausal Chinese women, neither serum cathepsin K nor CTSK gene polymorphisms were correlated with bone mineral density (BMD) or bone turnover markers [3]. Mutations in the CTSK gene are responsible for pycnodysostosis, a rare autosomal recessive skeletal dysplasia, as shown in research on patients from different ethnic groups [4,5,6,7,8,9].
In conclusion, Ctsk is crucial for bone-related processes, especially those involving extracellular matrix degradation and osteoclast-mediated bone resorption. Mouse models, like the Ctsk-Cre;Trp53f/f/Rb1f/f mice, have been instrumental in revealing its role in osteosarcoma development. Understanding Ctsk's function is important for diseases such as pycnodysostosis and potentially for CRPC, where it may serve as a therapeutic target [1,2,4,5,6,7,8,9].
References:
1. Wu, Ning, Wang, YouZhi, Wang, KeKe, Liang, JiaMing, Jiang, Ning. 2022. Cathepsin K regulates the tumor growth and metastasis by IL-17/CTSK/EMT axis and mediates M2 macrophage polarization in castration-resistant prostate cancer. In Cell death & disease, 13, 813. doi:10.1038/s41419-022-05215-8. https://pubmed.ncbi.nlm.nih.gov/36138018/
2. Li, Yang, Yang, Shuting, Yang, Shuying. 2022. Verteporfin Inhibits the Progression of Spontaneous Osteosarcoma Caused by Trp53 and Rb1 Deficiency in Ctsk-Expressing Cells via Impeding Hippo Pathway. In Cells, 11, . doi:10.3390/cells11081361. https://pubmed.ncbi.nlm.nih.gov/35456040/
3. Gao, Li-Hong, Li, Shan-Shan, Yue, Hua, Zhang, Zhen-Lin. 2020. Associations of Serum Cathepsin K and Polymorphisms in CTSK Gene With Bone Mineral Density and Bone Metabolism Markers in Postmenopausal Chinese Women. In Frontiers in endocrinology, 11, 48. doi:10.3389/fendo.2020.00048. https://pubmed.ncbi.nlm.nih.gov/32117071/
4. Markova, Tatiana Vladimirovna, Kenis, Vladimir, Melchenko, Evgeniy, Nagornova, Tatiana, Dadali, Elena Leonidovna. 2022. Clinical and genetic characterization of three Russian patients with pycnodysostosis due to pathogenic variants in the CTSK gene. In Molecular genetics & genomic medicine, 10, e1904. doi:10.1002/mgg3.1904. https://pubmed.ncbi.nlm.nih.gov/35315254/
5. Huang, Xianglan, Qi, Xuan, Li, Mei, Hu, Ying Ying, Xia, Weibo. 2015. A Mutation in CTSK Gene in an Autosomal Recessive Pycnodysostosis Family of Chinese Origin. In Calcified tissue international, 96, 373-8. doi:10.1007/s00223-015-9963-y. https://pubmed.ncbi.nlm.nih.gov/25725806/
6. Sait, Haseena, Srivastava, Priyanka, Gupta, Neerja, Pabbati, Jayalakshmi, Phadke, Shubha R. 2021. Phenotypic and genotypic spectrum of CTSK variants in a cohort of twenty-five Indian patients with pycnodysostosis. In European journal of medical genetics, 64, 104235. doi:10.1016/j.ejmg.2021.104235. https://pubmed.ncbi.nlm.nih.gov/33945887/
7. Xue, Y, Wang, L, Xia, D, Mao, T, Duan, X. 2015. Dental Abnormalities Caused by Novel Compound Heterozygous CTSK Mutations. In Journal of dental research, 94, 674-81. doi:10.1177/0022034515573964. https://pubmed.ncbi.nlm.nih.gov/25731711/
8. Naeem, Muhammad, Sheikh, Sabeen, Ahmad, Wasim. 2009. A mutation in CTSK gene in an autosomal recessive pycnodysostosis family of Pakistani origin. In BMC medical genetics, 10, 76. doi:10.1186/1471-2350-10-76. https://pubmed.ncbi.nlm.nih.gov/19674475/
9. Ho, N, Punturieri, A, Wilkin, D, Weiss, S, Francomano, C. . Mutations of CTSK result in pycnodysostosis via a reduction in cathepsin K protein. In Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 14, 1649-53. doi:. https://pubmed.ncbi.nlm.nih.gov/10491211/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen