C57BL/6JCya-Dclk1em1flox/Cya
Common Name:
Dclk1-flox
Product ID:
S-CKO-02003
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dclk1-flox
Strain ID
CKOCMP-13175-Dclk1-B6J-VA
Gene Name
Product ID
S-CKO-02003
Gene Alias
1700113D08Rik; 2810480F11Rik; Click-I; Cpg16; Dcamkl1; Dcl; Dclk; mKIAA0369
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dclk1em1flox/Cya mice (Catalog S-CKO-02003) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000054237
NCBI RefSeq
NM_019978
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Dclk1, or Doublecortin-like kinase 1, is a microtubule-associated protein kinase. It is involved in neurogenesis and has been linked to multiple important pathways like Notch, Wnt/β -catenin, and RAS, playing a significant role in various biological processes and diseases [3,4,6]. It is also recognized as a marker for certain cell types, such as tuft cells in the gastrointestinal tract and a biomarker for cancer stem cells in several cancers [3,4,5,6,7,8,9].
In atherosclerosis, macrophage-specific Dclk1 deletion in ApoE-/-mice fed a high-fat diet (HFD) attenuates the disease by reducing inflammation. Mechanistically, Dclk1 in macrophages binds to IKKβ, phosphorylates it at S177/181, activates the NF-κB signaling pathway, and induces inflammatory gene expression [1]. In obesity-induced cardiomyopathy, macrophage-specific Dclk1 knockout, rather than cardiomyocyte-specific knockout, prevents HFD-induced heart dysfunction, cardiac hypertrophy, and fibrosis. Dclk1 deficiency exerts cardioprotective effects by suppressing RIP2/TAK1 activation and inflammatory responses in macrophages [2].
In conclusion, Dclk1 plays crucial roles in inflammation-related cardiovascular diseases like atherosclerosis and obesity-induced cardiomyopathy. The use of Dclk1 knockout (KO) or conditional knockout (CKO) mouse models has been instrumental in revealing its functions in these disease conditions, highlighting Dclk1 as a potential therapeutic target for these diseases.
References:
1. Huang, Zhuqi, Shen, Sirui, Han, Xue, Wu, Gaojun, Liang, Guang. 2023. Macrophage DCLK1 promotes atherosclerosis via binding to IKKβ and inducing inflammatory responses. In EMBO molecular medicine, 15, e17198. doi:10.15252/emmm.202217198. https://pubmed.ncbi.nlm.nih.gov/36896602/
2. Yang, Bin, Zhao, Yunjie, Luo, Wu, Wang, Yi, Liang, Guang. 2023. Macrophage DCLK1 promotes obesity-induced cardiomyopathy via activating RIP2/TAK1 signaling pathway. In Cell death & disease, 14, 419. doi:10.1038/s41419-023-05960-4. https://pubmed.ncbi.nlm.nih.gov/37443105/
3. Lu, Qin, Feng, Hailan, Chen, Hong, Yan, Zixing, Cao, Zhiyun. 2022. Role of DCLK1 in oncogenic signaling (Review). In International journal of oncology, 61, . doi:10.3892/ijo.2022.5427. https://pubmed.ncbi.nlm.nih.gov/36148883/
4. Ye, Liu, Liu, Beibei, Huang, Jingling, Xu, Yungen, Wang, Shuping. 2023. DCLK1 and its oncogenic functions: A promising therapeutic target for cancers. In Life sciences, 336, 122294. doi:10.1016/j.lfs.2023.122294. https://pubmed.ncbi.nlm.nih.gov/38007147/
5. Ding, Ling, Weygant, Nathaniel, Ding, Chenhuan, Lai, Yi, Li, He. 2023. DCLK1 and tuft cells: Immune-related functions and implications for cancer immunotherapy. In Critical reviews in oncology/hematology, 191, 104118. doi:10.1016/j.critrevonc.2023.104118. https://pubmed.ncbi.nlm.nih.gov/37660932/
6. Vijai, Muthu, Baba, Mursaleen, Ramalingam, Satish, Thiyagaraj, Anand. 2021. DCLK1 and its interaction partners: An effective therapeutic target for colorectal cancer. In Oncology letters, 22, 850. doi:10.3892/ol.2021.13111. https://pubmed.ncbi.nlm.nih.gov/34733368/
7. Cheng, Linna, Huang, Shenzhen, Chen, Lijuan, Zhu, Zunmin, Thorne, Rick F. . Research Progress of DCLK1 Inhibitors as Cancer Therapeutics. In Current medicinal chemistry, 29, 2261-2273. doi:10.2174/0929867328666210709110721. https://pubmed.ncbi.nlm.nih.gov/34254905/
8. Chhetri, Dibyashree, Vengadassalapathy, Srinivasan, Venkadassalapathy, Santhosh, Palaniyandi, Kanagaraj, Gnanasampanthapandian, Dhanavathy. 2022. Pleiotropic effects of DCLK1 in cancer and cancer stem cells. In Frontiers in molecular biosciences, 9, 965730. doi:10.3389/fmolb.2022.965730. https://pubmed.ncbi.nlm.nih.gov/36250024/
9. Wang, Yifan, Yi, Jun, Liu, Xiaowei. 2021. Roles of Dclk1 in the pathogenesis, diagnosis, prognosis and treatment of pancreatic cancer: A review. In Expert review of gastroenterology & hepatology, 16, 13-19. doi:10.1080/17474124.2022.2020643. https://pubmed.ncbi.nlm.nih.gov/34937474/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen