C57BL/6JCya-Fabp3em1flox/Cya
Common Name:
Fabp3-flox
Product ID:
S-CKO-02338
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Fabp3-flox
Strain ID
CKOCMP-14077-Fabp3-B6J-VA
Gene Name
Product ID
S-CKO-02338
Gene Alias
Fabph-1; Fabph-4; Fabph1; Fabph4; H-FABP; Mdgi
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fabp3em1flox/Cya mice (Catalog S-CKO-02338) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000070532
NCBI RefSeq
NM_010174
Target Region
Exon 2~3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Fabp3, also known as fatty acid binding protein 3 (muscle and heart), is a cytoplasmic fatty acid binding protein expressed in a tissue-specific manner, predominantly in breast, muscle, and heart. It is involved in various biological processes such as lipid metabolism and transport. By binding fatty acids, it may play a role in intracellular trafficking of lipids, which is crucial for normal cellular function and energy homeostasis [3].
In aged skeletal muscles, FABP3 is upregulated, disrupting homeostasis via lipid remodeling. FABP3 overexpression in young muscles mimics the aged-muscle lipid composition, causing ER stress through the PERK-eIF2α pathway and inhibiting protein synthesis, while FABP3 knockdown in aged muscles reduces ER stress and improves muscle recovery [1].
In mesenchymal stem cells, FABP3 promotes adipogenesis and inhibits osteogenesis. Knockdown of FABP3 alleviates bone loss in aged mice, indicating its role in the bone-fat balance during aging [2].
In the context of cerebral ischemia-reperfusion injury, FABP3 silencing in mice mitigates brain tissue damage, reduces infarct volume ratio, and attenuates neuronal apoptosis, as FABP3 activates mitochondrial autophagy through ROS, leading to mitochondrial dysfunction and neuronal apoptosis [4].
In conclusion, Fabp3 plays essential roles in multiple biological processes, including muscle homeostasis during aging, mesenchymal stem cell fate determination, and the progression of cerebral ischemia-reperfusion injury. The use of gene knockout models in these studies has provided valuable insights into the functions of Fabp3 in these specific disease-related biological processes, highlighting its potential as a therapeutic target for sarcopenia, senile osteoporosis, and stroke-related brain injury.
References:
1. Lee, Seung-Min, Lee, Seol Hee, Jung, Youngae, Lee, Kwang-Pyo, Kwon, Ki-Sun. 2020. FABP3-mediated membrane lipid saturation alters fluidity and induces ER stress in skeletal muscle with aging. In Nature communications, 11, 5661. doi:10.1038/s41467-020-19501-6. https://pubmed.ncbi.nlm.nih.gov/33168829/
2. Liu, Zheng-Zhao, Hong, Chun-Gu, Hu, Wen-Bao, Hu, Rong-Gui, Xie, Hui. 2020. Autophagy receptor OPTN (optineurin) regulates mesenchymal stem cell fate and bone-fat balance during aging by clearing FABP3. In Autophagy, 17, 2766-2782. doi:10.1080/15548627.2020.1839286. https://pubmed.ncbi.nlm.nih.gov/33143524/
3. Baru, Ambica, Devi, Chandra, Mukhopadhyay, Tapas, Dogra, Nilambra. 2023. Anti bacterial function of secreted human FABP3. In Biochimica et biophysica acta. General subjects, 1867, 130472. doi:10.1016/j.bbagen.2023.130472. https://pubmed.ncbi.nlm.nih.gov/37788726/
4. Zhong, Fang-Fang, Wei, Bo, Bao, Guo-Xiang, Xiao, Xiao, Tian, Jin-Jin. 2024. FABP3 Induces Mitochondrial Autophagy to Promote Neuronal Cell Apoptosis in Brain Ischemia-Reperfusion Injury. In Neurotoxicity research, 42, 35. doi:10.1007/s12640-024-00712-4. https://pubmed.ncbi.nlm.nih.gov/39008165/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen