C57BL/6JCya-Xrcc6em1flox/Cya
Common Name:
Xrcc6-flox
Product ID:
S-CKO-02517
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Xrcc6-flox
Strain ID
CKOCMP-14375-Xrcc6-B6J-VA
Gene Name
Product ID
S-CKO-02517
Gene Alias
70kDa; G22p1; Ku70
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Xrcc6em1flox/Cya mice (Catalog S-CKO-02517) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000069530
NCBI RefSeq
NM_010247
Target Region
Exon 4~5
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
XRCC6, also known as Ku70, is a gene encoding a protein that plays a crucial role in the DNA non-homologous end-joining (NHEJ) pathway, which is the main mechanism for repairing DNA double-strand breaks (DSBs) in human cells. Maintaining genomic stability by repairing DSBs is essential in preventing tumor initiation and progression, thus XRCC6 is of great biological importance in carcinogenesis [2].
Multiple studies have investigated the association between XRCC6 polymorphisms and cancer risks. A meta-analysis showed that the rs2267437 polymorphism was associated with an increased risk of overall cancers, breast cancer, renal cell carcinoma, and hepatocellular carcinoma, especially in Asian populations [1]. Another study on nasopharyngeal carcinoma (NPC) found that the TC and CC genotypes of the XRCC6 promoter T-991C polymorphism were associated with a significantly increased NPC risk, and lower XRCC6 mRNA and protein expression was observed in NPC samples with these genotypes [3]. In a Taiwanese population, similar results were found for hepatocellular carcinoma (HCC), where the TC and CC genotypes of the XRCC6 promoter T-991C polymorphism increased the HCC risk, along with lower XRCC6 mRNA and protein expressions [4]. In esophageal squamous cell carcinoma (ESCC), the CG carriers and G allele carriers of rs2267437 were at a higher risk of ESCC [5]. In contrast, in the Polish population, the XRCC6 C1310G polymorphism was associated with a decreased risk of colorectal cancer [6]. Additionally, high expression of XRCC6 promoted human osteosarcoma cell proliferation through the β-catenin/Wnt signaling pathway [7].
In conclusion, XRCC6 is crucial for DNA double-strand break repair through the NHEJ pathway, which is significant in maintaining genomic stability and preventing carcinogenesis. Studies on XRCC6 polymorphisms have revealed its associations with various cancers, such as breast, renal cell, hepatocellular, nasopharyngeal, esophageal squamous cell, and colorectal cancers, as well as osteosarcoma, providing insights into the role of XRCC6 in different disease conditions.
References：
1. Jia, Jing, Ren, Juan, Yan, Dongmei, Xiao, Long, Sun, Ruifen. . Association between the XRCC6 polymorphisms and cancer risks: a systematic review and meta-analysis. In Medicine, 94, e283. doi:10.1097/MD.0000000000000283. https://pubmed.ncbi.nlm.nih.gov/25569644/
2. Bau, Da-Tian, Tsai, Chia-Wen, Wu, Cheng-Nan. . Role of the XRCC5/XRCC6 dimer in carcinogenesis and pharmacogenomics. In Pharmacogenomics, 12, 515-34. doi:10.2217/pgs.10.209. https://pubmed.ncbi.nlm.nih.gov/21521024/
3. Huang, C-Y, Tsai, C-W, Hsu, C-M, Shui, H-A, Bau, D-T. 2015. The role of XRCC6/Ku70 in nasopharyngeal carcinoma. In International journal of oral and maxillofacial surgery, 44, 1480-5. doi:10.1016/j.ijom.2015.06.008. https://pubmed.ncbi.nlm.nih.gov/26149939/
4. Hsu, Chin-Mu, Yang, Mei-Due, Chang, Wen-Shin, Tsai, Fuu-Jen, Bau, Da-Tian. . The contribution of XRCC6/Ku70 to hepatocellular carcinoma in Taiwan. In Anticancer research, 33, 529-35. doi:. https://pubmed.ncbi.nlm.nih.gov/23393345/
5. Li, Kun, Yin, Xiuli, Yang, Hongli, Xu, Changqing, Xu, Hongwei. 2015. Association of the genetic polymorphisms in XRCC6 and XRCC5 with the risk of ESCC in a high-incidence region of North China. In Tumori, 101, 24-9. doi:10.5301/tj.5000206. https://pubmed.ncbi.nlm.nih.gov/25702660/
6. Balinska, Kinga, Wilk, Damian, Filipek, Beata, Kabziński, Jacek, Majsterek, Ireneusz. . Association of XRCC6 C1310G and LIG4 T9I polymorphisms of NHEJ DNA repair pathway with risk of colorectal cancer in the Polish population. In Polski przeglad chirurgiczny, 91, 15-20. doi:10.5604/01.3001.0013.1030. https://pubmed.ncbi.nlm.nih.gov/31243170/
7. Zhu, Bin, Cheng, Dongdong, Li, Shijie, Zhou, Shumin, Yang, Qingcheng. 2016. High Expression of XRCC6 Promotes Human Osteosarcoma Cell Proliferation through the β-Catenin/Wnt Signaling Pathway and Is Associated with Poor Prognosis. In International journal of molecular sciences, 17, . doi:10.3390/ijms17071188. https://pubmed.ncbi.nlm.nih.gov/27455247/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen