C57BL/6JCya-Kcnmb1em1flox/Cya
Common Name:
Kcnmb1-flox
Product ID:
S-CKO-03240
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kcnmb1-flox
Strain ID
CKOCMP-16533-Kcnmb1-B6J-VA
Gene Name
Product ID
S-CKO-03240
Gene Alias
BKbeta1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kcnmb1em1flox/Cya mice (Catalog S-CKO-03240) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020362
NCBI RefSeq
NM_031169.4
Target Region
Exon 2~3
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
KCNMB1, encoding the β1 subunit of the large-conductance calcium-activated potassium (BK) channel, is crucial for regulating BK channel activity. The BK channels are involved in various physiological processes such as smooth muscle contraction, neurotransmitter release, and regulation of vascular tone [2,3,4,5,6,7,8,9]. Genetic models can help understand its function and its role in related biological pathways and diseases.
In a meta-analysis, the dominant model of the KCNMB1 E65K polymorphism was significantly associated with essential hypertension risk in Asians, suggesting it might be a susceptibility factor [1]. In Kcnmb1-/-mice, they are hypertensive, volume expanded, and have reduced urinary K and Na clearances, with the majority of hypertension due to aldosteronism from renal potassium retention and hyperkalemia [7]. Fibroblasts from patients with idiopathic pulmonary fibrosis (IPF) show increased KCNMB1 expression, and knockdown of KCNMB1 attenuates their ability to contract collagen gels, indicating a role in myofibroblast differentiation [2].
In conclusion, KCNMB1 plays a vital role in regulating BK channel activity, which impacts various physiological functions. Studies using KO mouse models have revealed its significance in diseases like hypertension and pulmonary fibrosis. Understanding KCNMB1 through these models provides insights into disease mechanisms and potential therapeutic targets for these conditions.
References:
1. Zhancheng, Wang, Wenhui, Ji, Yun, Jiang, Yan, Shen, Jin, Li. . The dominant models of KCNJ11 E23K and KCNMB1 E65K are associated with essential hypertension (EH) in Asian: Evidence from a meta-analysis. In Medicine, 98, e15828. doi:10.1097/MD.0000000000015828. https://pubmed.ncbi.nlm.nih.gov/31169684/
2. Scruggs, Anne M, Grabauskas, Gintautas, Huang, Steven K. . The Role of KCNMB1 and BK Channels in Myofibroblast Differentiation and Pulmonary Fibrosis. In American journal of respiratory cell and molecular biology, 62, 191-203. doi:10.1165/rcmb.2019-0163OC. https://pubmed.ncbi.nlm.nih.gov/31486669/
3. Bukiya, Anna N, Leo, M Dennis, Jaggar, Jonathan H, Dopico, Alex M. 2021. Cholesterol activates BK channels by increasing KCNMB1 protein levels in the plasmalemma. In The Journal of biological chemistry, 296, 100381. doi:10.1016/j.jbc.2021.100381. https://pubmed.ncbi.nlm.nih.gov/33556372/
4. Son, Yonghae, Chun, Wonjoo, Ahn, Yong-Tae, Lee, Chu, An, Won G. 2015. 7-Ketocholesterol induces the reduction of KCNMB1 in atherosclerotic blood vessels. In Biochemical and biophysical research communications, 457, 324-7. doi:10.1016/j.bbrc.2014.12.109. https://pubmed.ncbi.nlm.nih.gov/25576871/
5. Mysiewicz, Steven, North, Kelsey C, Moreira, Luiz, Bukiya, Anna N, Dopico, Alex M. 2023. Interspecies and regional variability of alcohol action on large cerebral arteries: regulation by KCNMB1 proteins. In American journal of physiology. Regulatory, integrative and comparative physiology, 324, R480-R496. doi:10.1152/ajpregu.00103.2022. https://pubmed.ncbi.nlm.nih.gov/36717168/
6. Beitelshees, Amber L, Gong, Yan, Wang, Danxin, Pepine, Carl J, Johnson, Julie A. . KCNMB1 genotype influences response to verapamil SR and adverse outcomes in the INternational VErapamil SR/Trandolapril STudy (INVEST). In Pharmacogenetics and genomics, 17, 719-29. doi:. https://pubmed.ncbi.nlm.nih.gov/17700361/
7. Grimm, P Richard, Irsik, Debra L, Settles, Deann C, Holtzclaw, J David, Sansom, Steven C. 2009. Hypertension of Kcnmb1-/- is linked to deficient K secretion and aldosteronism. In Proceedings of the National Academy of Sciences of the United States of America, 106, 11800-5. doi:10.1073/pnas.0904635106. https://pubmed.ncbi.nlm.nih.gov/19556540/
8. Toro, B, Cox, N, Wilson, R J, Toro, L, Zarei, M M. 2006. KCNMB1 regulates surface expression of a voltage and Ca2+-activated K+ channel via endocytic trafficking signals. In Neuroscience, 142, 661-9. doi:. https://pubmed.ncbi.nlm.nih.gov/16908104/
9. Ahn, Yong-Tae, Kim, Yu-Mee, Adams, Eloa, Alvira, Cristina M, Cornfield, David N. 2011. Hypoxia-inducible factor-1α regulates KCNMB1 expression in human pulmonary artery smooth muscle cells. In American journal of physiology. Lung cellular and molecular physiology, 302, L352-9. doi:10.1152/ajplung.00302.2011. https://pubmed.ncbi.nlm.nih.gov/22114151/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen