C57BL/6JCya-Kif11em1flox/Cya
Common Name:
Kif11-flox
Product ID:
S-CKO-03251
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kif11-flox
Strain ID
CKOCMP-16551-Kif11-B6J-VA
Gene Name
Product ID
S-CKO-03251
Gene Alias
Eg5; Kif8; Kifl1; Knsl1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kif11em1flox/Cya mice (Catalog S-CKO-03251) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000012587
NCBI RefSeq
NM_010615
Target Region
Exon 2~4
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
KIF11, a member of the kinesin family, plays a vital role in the formation and maintenance of the mitotic spindle, thus being crucial for the accurate separation of daughter cells during mitosis [3]. It is also involved in regulating the cell cycle and is associated with many biological processes and diseases. Genetic models, such as gene knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying its functions.
In photoreceptor cells, KIF11 localizes at the transition zone of the photoreceptor cilium. KIF11 depletion leads to malformations of the ciliary axoneme and membranous discs, resulting in photoreceptor degeneration and drusen-like deposit accumulation in the retina [1]. In hepatocellular carcinoma, knockdown of KIF11 causes cellular senescence and inhibits tumor progression, associated with increased expression of CDKN2A [2]. In endometrial cancer, KIF11 knockdown inhibits cell proliferation, migration, and invasion, and induces G2/M phase arrest and apoptosis [4]. Similar inhibitory effects on cancer cell growth and progression are seen in thyroid cancer, pancreatic ductal adenocarcinoma, non-small cell lung cancer, adrenocortical carcinoma, and childhood acute lymphoblastic leukemia when KIF11 is knocked down or inhibited [5,6,7,8,9].
In conclusion, KIF11 is essential for mitosis and cell cycle regulation. Model-based research, especially KO/CKO mouse models, has revealed its significant roles in maintaining photoreceptor cilium integrity and in the progression of multiple cancers. Understanding KIF11's functions provides potential prognostic biomarkers and therapeutic targets for related diseases.
References:
1. Ran, Jie, Guo, Guizhi, Zhang, Sai, Zhou, Jun, Liu, Min. 2024. KIF11 UFMylation Maintains Photoreceptor Cilium Integrity and Retinal Homeostasis. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2400569. doi:10.1002/advs.202400569. https://pubmed.ncbi.nlm.nih.gov/38666385/
2. Chen, Danlei, Wang, Jinghao, Li, Yang, Zhang, Pengfei, Liu, Lianxin. . LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11-dependent repression of CDKN2A. In Clinical and translational medicine, 13, e1418. doi:10.1002/ctm2.1418. https://pubmed.ncbi.nlm.nih.gov/37752791/
3. Gao, Wanting, Lu, Junjie, Yang, Zitao, Cao, Yufei, Xie, Lei. 2024. Mitotic Functions and Characters of KIF11 in Cancers. In Biomolecules, 14, . doi:10.3390/biom14040386. https://pubmed.ncbi.nlm.nih.gov/38672404/
4. Wang, Biying, Bao, Lunmin, Li, Xiaoduo, Yuan, Rui, Jiang, Hongmei. 2025. Identification and validation of the important role of KIF11 in the development and progression of endometrial cancer. In Journal of translational medicine, 23, 48. doi:10.1186/s12967-025-06081-6. https://pubmed.ncbi.nlm.nih.gov/39806429/
5. Han, Yue, Chen, Jing, Wei, Dianjun, Wang, Baoxi. 2022. KIF11 Is a Promising Therapeutic Target for Thyroid Cancer Treatment. In Computational and mathematical methods in medicine, 2022, 6426800. doi:10.1155/2022/6426800. https://pubmed.ncbi.nlm.nih.gov/36017147/
6. Gu, Xiang, Zhu, Qunshan, Tian, Guangyu, Chen, Xiaojun, Qin, Songbing. 2022. KIF11 manipulates SREBP2-dependent mevalonate cross talk to promote tumor progression in pancreatic ductal adenocarcinoma. In Cancer medicine, 11, 3282-3295. doi:10.1002/cam4.4683. https://pubmed.ncbi.nlm.nih.gov/35619540/
7. Liu, Junhui, Tian, Yubin, Yi, Lei, Lou, Ming, Yuan, Kai. 2021. High KIF11 expression is associated with poor outcome of NSCLC. In Tumori, 108, 40-46. doi:10.1177/0300891620988342. https://pubmed.ncbi.nlm.nih.gov/33554761/
8. Zhou, Yan, Chen, Xiang, Li, Bingsheng, Li, Yang, Zhang, Bo. 2023. KIF11 is a potential prognostic biomarker and therapeutic target for adrenocortical carcinoma. In Translational andrology and urology, 12, 594-611. doi:10.21037/tau-22-706. https://pubmed.ncbi.nlm.nih.gov/37181234/
9. Zhu, Liwen, Chen, Chuqin, Kang, Meiyun, Xue, Yao, Fang, Yongjun. 2023. KIF11 serves as a cell cycle mediator in childhood acute lymphoblastic leukemia. In Journal of cancer research and clinical oncology, 149, 15609-15622. doi:10.1007/s00432-023-05240-w. https://pubmed.ncbi.nlm.nih.gov/37656243/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen