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C57BL/6JCya-Arhgef2em1flox/Cya
Common Name:
Arhgef2-flox
Product ID:
S-CKO-03357
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Arhgef2-flox
Strain ID
CKOCMP-16800-Arhgef2-B6J-VA
Gene Name
Arhgef2
Product ID
S-CKO-03357
Gene Alias
GEF; GEF-H1; GEFH1; LFP40; Lbcl1; Lfc; P40; mKIAA0651
Background
C57BL/6JCya
NCBI ID
16800
Modification
Conditional knockout
Chromosome
3
Phenotype
MGI:103264
Document
Click here to download >>
Application
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Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Arhgef2em1flox/Cya mice (Catalog S-CKO-03357) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029694
NCBI RefSeq
NM_008487.4
Target Region
Exon 5~9
Size of Effective Region
~2.8 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Arhgef2, also known as Rho/Rac guanine nucleotide exchange factor 2, is a key regulator that activates RhoA GTPase, thereby modulating cytoskeleton dynamics, cell division, and cell migration [5,6,7]. It is involved in multiple biological pathways, such as the RhoA/ROCK/MLC pathway crucial for cell migration [7]. Arhgef2 is of great biological importance in processes like neural differentiation, hematopoiesis, and tumorigenesis [3,5,7]. Genetic models, especially knockout mouse models, have been valuable in studying its functions.

In animal models, Arhgef2 deficiency leads to impaired neurogenesis, neurite outgrowth, and synaptic formation in the cerebral cortex by regulating m6A methylation of Npdc1 and Cend1 [3]. In hematopoietic stem cells, Arhgef2 knockout results in impaired hematopoietic recovery and a production deficit, along with an increased frequency of misoriented divisions [5]. In colorectal cancer, YTHDF1 promotes ARHGEF2 translation, and ARHGEF2 siRNA encapsulated by lipid nanoparticles significantly suppresses tumor growth and metastasis in vivo [1]. In prostate cancer, androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation [2]. In hepatocellular carcinoma, ARHGEF2 overexpression is linked to malignant development, and it contributes to ER stress-related treatment resistance [4].

In summary, Arhgef2 is essential for neural differentiation, hematopoiesis, and normal cellular functions. Its dysregulation is associated with various diseases, including neurodevelopmental disorders, hematopoietic failures, and multiple types of cancers. Gene knockout mouse models have provided significant insights into Arhgef2's role in these disease areas, facilitating a better understanding of the underlying molecular mechanisms and potentially guiding the development of targeted therapies.

References:
1. Wang, Shiyan, Gao, Shanshan, Zeng, Yong, Yu, Jun, He, Housheng Hansen. 2021. N6-Methyladenosine Reader YTHDF1 Promotes ARHGEF2 Translation and RhoA Signaling in Colorectal Cancer. In Gastroenterology, 162, 1183-1196. doi:10.1053/j.gastro.2021.12.269. https://pubmed.ncbi.nlm.nih.gov/34968454/
2. Chen, Xuanrong, Shao, Yi, Wei, Wanqing, Niu, Yuanjie, Shang, Zhiqun. 2022. Androgen deprivation restores ARHGEF2 to promote neuroendocrine differentiation of prostate cancer. In Cell death & disease, 13, 927. doi:10.1038/s41419-022-05366-8. https://pubmed.ncbi.nlm.nih.gov/36335093/
3. Zhou, Pei, Qi, Yifei, Fang, Xiang, Kaindl, Angela M, Hu, Hao. 2021. Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA m6A-methylation of Npdc1 and Cend1. In iScience, 24, 102645. doi:10.1016/j.isci.2021.102645. https://pubmed.ncbi.nlm.nih.gov/34142067/
4. Zhu, Yue, Liu, Weiwei, Wang, Zishu, Liu, Jiatao, Sun, Guoping. 2022. ARHGEF2/EDN1 pathway participates in ER stress-related drug resistance of hepatocellular carcinoma by promoting angiogenesis and malignant proliferation. In Cell death & disease, 13, 652. doi:10.1038/s41419-022-05099-8. https://pubmed.ncbi.nlm.nih.gov/35896520/
5. Chan, Derek C H, Xu, Joshua, Vujovic, Ana, Rottapel, Robert K, Hope, Kristin J. . Arhgef2 regulates mitotic spindle orientation in hematopoietic stem cells and is essential for productive hematopoiesis. In Blood advances, 5, 3120-3133. doi:10.1182/bloodadvances.2020002539. https://pubmed.ncbi.nlm.nih.gov/34406376/
6. Olivier, Jean-Frederic, Langlais, David, Jeyakumar, Thiviya, Gros, Philippe, Fodil, Nassima. 2024. CCDC88B interacts with RASAL3 and ARHGEF2 and regulates dendritic cell function in neuroinflammation and colitis. In Communications biology, 7, 77. doi:10.1038/s42003-023-05751-9. https://pubmed.ncbi.nlm.nih.gov/38200184/
7. Ravindran, Ethiraj, Hu, Hao, Yuzwa, Scott A, Hübner, Christoph, Kaindl, Angela M. 2017. Homozygous ARHGEF2 mutation causes intellectual disability and midbrain-hindbrain malformation. In PLoS genetics, 13, e1006746. doi:10.1371/journal.pgen.1006746. https://pubmed.ncbi.nlm.nih.gov/28453519/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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