C57BL/6JCya-Lta4hem1flox/Cya
Common Name:
Lta4h-flox
Product ID:
S-CKO-03439
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Lta4h-flox
Strain ID
CKOCMP-16993-Lta4h-B6J-VA
Gene Name
Product ID
S-CKO-03439
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lta4hem1flox/Cya mice (Catalog S-CKO-03439) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000016033
NCBI RefSeq
NM_008517
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Lta4h, short for leukotriene A4 hydrolase, is a bifunctional zinc metallo-enzyme. It is classically known for its epoxide hydrolase activity, converting leukotriene A4 to the neutrophil chemoattractant LTB4. It is also involved in the metabolism of polyunsaturated fatty acid (PUFA), specifically in the arachidonic acid (AA) metabolism pathway. Lta4h has been implicated in the regulation of various biological processes and is associated with many diseases, such as chronic lung diseases and cancers [1,2,3,4,5,6].
In hepatocellular carcinoma (HCC), Lta4h is downregulated. Lta4h-deficient mice show exacerbated hepatocyte damage by restraining JNK activation and increased CD206+ macrophage polarization via upregulation of LTBP1 and downstream TGF-β secretion. Mechanistically, Lta4h induces HNRNPA1 phosphorylation, affecting Ltbp1 mRNA maturation. Lta4h-deficient patients have poor prognosis and immunotherapy resistance [1]. In skin cancer, Lta4h knockout significantly reduces skin cancer development in a mouse model. Lta4h depletion decreases skin cancer cell growth by inducing cell cycle arrest at the G0/G1 phase through enhancing p27 protein stability [4]. In ovarian cancer, Lta4h deficiency enhances sensitivity to Cisplatin and modifies immune cell infiltration characteristics [5].
In conclusion, Lta4h plays crucial roles in multiple biological processes, especially in cell cycle regulation and immune microenvironment modulation. Gene knockout mouse models have revealed its significance in cancer development and treatment response, providing insights into potential diagnostic and therapeutic strategies for Lta4h-related diseases, particularly in HCC, skin cancer, and ovarian cancer.
References:
1. Yang, Shuai, Qiu, Xinyao, Yang, Yingcheng, Wang, Hongyang, Chen, Lei. 2025. LTA4H improves the tumor microenvironment and prevents HCC progression via targeting the HNRNPA1/LTBP1/TGF-β axis. In Cell reports. Medicine, 6, 102000. doi:10.1016/j.xcrm.2025.102000. https://pubmed.ncbi.nlm.nih.gov/40056904/
2. Ren, Tianjiao, Wang, Song, Zhang, Bo, Zhao, Xiaorui, Feng, Juan. 2023. LTA4H extensively associates with mRNAs and lncRNAs indicative of its novel regulatory targets. In PeerJ, 11, e14875. doi:10.7717/peerj.14875. https://pubmed.ncbi.nlm.nih.gov/36923505/
3. Loesche, Christian, Picard, Damien, Van Hoorick, Benjamin, Fink, Martin, Röhn, Till A. . LTA4H inhibitor LYS006: Clinical PK/PD and safety in a randomized phase I clinical trial. In Clinical and translational science, 17, e13724. doi:10.1111/cts.13724. https://pubmed.ncbi.nlm.nih.gov/38407540/
4. Oi, Naomi, Yamamoto, Hiroyuki, Langfald, Alyssa, Bode, Ann M, Dong, Zigang. . LTA4H regulates cell cycle and skin carcinogenesis. In Carcinogenesis, 38, 728-737. doi:10.1093/carcin/bgx049. https://pubmed.ncbi.nlm.nih.gov/28575166/
5. Guo, Zhengyang, Huang, Jiaqi, Huo, Xiao, Yang, Jianling, Xue, Lixiang. 2024. Targeting LTA4H facilitates the reshaping of the immune microenvironment mediated by CCL5 and sensitizes ovarian cancer to Cisplatin. In Science China. Life sciences, 67, 1226-1241. doi:10.1007/s11427-023-2444-5. https://pubmed.ncbi.nlm.nih.gov/38300441/
6. Zhao, Simin, Yao, Ke, Li, Dan, Guo, Zhiping, Dong, Zigang. 2019. Inhibition of LTA4H by bestatin in human and mouse colorectal cancer. In EBioMedicine, 44, 361-374. doi:10.1016/j.ebiom.2019.05.008. https://pubmed.ncbi.nlm.nih.gov/31085102/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen