C57BL/6JCya-Acot4em1flox/Cya
Common Name:
Acot4-flox
Product ID:
S-CKO-03614
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Acot4-flox
Strain ID
CKOCMP-171282-Acot4-B6J-VA
Gene Name
Product ID
S-CKO-03614
Gene Alias
B430212I04Rik; PTE-Ib; Pte1b; Pte2b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acot4em1flox/Cya mice (Catalog S-CKO-03614) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021652
NCBI RefSeq
NM_134247
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Acot4, an isoform of the acyl-CoA thioesterase (ACOT) family, catalyzes the hydrolysis of acyl-CoA thioesters to non-esterified fatty acid and coenzyme A (CoA) [1,3,5,7]. It is involved in lipid metabolism pathways and has been shown to be important in maintaining normal cellular lipid homeostasis [1,2,4,6,7]. Genetic models, such as knockout (KO) or conditional knockout (CKO) mouse models, can be valuable in further exploring its functions.
Depletion of Acot4 attenuated the proliferation and tumour formation of pancreatic ductal carcinoma (PDAC) cells, and its expression in PDAC was negatively correlated with patient survival, indicating its role in pancreatic tumourigenesis [1]. In hepatocytes, Acot4 knockdown alleviated gluconeogenesis and lipid accumulation, suggesting its relation to hepatic glucose and lipid metabolism, potentially via regulation of AMP-activated protein kinase (AMPK) activity [2]. In early-weaned rats, Acot4 expression was influenced by early weaning, which disrupted lipid metabolism, and leucine supplementation that alleviated metabolic disorders may have affected Acot4-related lipid metabolic processes [4].
In conclusion, Acot4 plays essential roles in lipid metabolism, including in tumourigenesis, hepatic metabolism, and early-life programming of obesity. The study of Acot4 using KO or CKO mouse models has provided insights into its functions in these disease-related areas, helping to understand the underlying mechanisms and potentially offering new directions for treatment and prevention.
References:
1. Ni, Chenming, Zheng, Kailian, Gao, Yunshu, Jin, Gang, Yu, Guanzhen. 2020. ACOT4 accumulation via AKT-mediated phosphorylation promotes pancreatic tumourigenesis. In Cancer letters, 498, 19-30. doi:10.1016/j.canlet.2020.09.022. https://pubmed.ncbi.nlm.nih.gov/33148467/
2. Yuan, Qianqian, Zhang, Xiaomin, Yang, Xiaonan, Liu, Shengxiu, Zhang, Huabing. 2024. Knockdown of ACOT4 alleviates gluconeogenesis and lipid accumulation in hepatocytes. In Heliyon, 10, e27618. doi:10.1016/j.heliyon.2024.e27618. https://pubmed.ncbi.nlm.nih.gov/38495177/
3. Li, Qing, Yang, Yu'e, Jiang, Xin, Mao, Yong, Hua, Dong. 2019. The combined expressions of B7H4 and ACOT4 in cancer-associated fibroblasts are related to poor prognosis in patients with gastric carcinoma. In International journal of clinical and experimental pathology, 12, 2672-2681. doi:. https://pubmed.ncbi.nlm.nih.gov/31934097/
4. Sun, Yuchen, Sun, Bo, Han, Xuesong, Shan, Anshan, Ma, Qingquan. . Leucine Supplementation Ameliorates Early-Life Programming of Obesity in Rats. In Diabetes, 72, 1409-1423. doi:10.2337/db22-0862. https://pubmed.ncbi.nlm.nih.gov/37196349/
5. Westin, Maria A K, Hunt, Mary C, Alexson, Stefan E H. 2005. The identification of a succinyl-CoA thioesterase suggests a novel pathway for succinate production in peroxisomes. In The Journal of biological chemistry, 280, 38125-32. doi:. https://pubmed.ncbi.nlm.nih.gov/16141203/
6. Li, Hongzhi, Li, Xiang, Yu, Shanshan, Sun, Xinyi, Zhao, Binghai. 2021. miR-23b Ameliorates nonalcoholic steatohepatitis by targeting Acyl-CoA thioesterases 4. In Experimental cell research, 407, 112787. doi:10.1016/j.yexcr.2021.112787. https://pubmed.ncbi.nlm.nih.gov/34450119/
7. Hunt, Mary C, Siponen, Marina I, Alexson, Stefan E H. 2012. The emerging role of acyl-CoA thioesterases and acyltransferases in regulating peroxisomal lipid metabolism. In Biochimica et biophysica acta, 1822, 1397-410. doi:10.1016/j.bbadis.2012.03.009. https://pubmed.ncbi.nlm.nih.gov/22465940/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen