C57BL/6JCya-Mdh2em1flox/Cya
Common Name:
Mdh2-flox
Product ID:
S-CKO-03768
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Mdh2-flox
Strain ID
CKOCMP-17448-Mdh2-B6J-VA
Gene Name
Product ID
S-CKO-03768
Gene Alias
MDH; Mdh-2; Mor-1; Mor1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mdh2em1flox/Cya mice (Catalog S-CKO-03768) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019323
NCBI RefSeq
NM_008617
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Mdh2, or malate dehydrogenase 2, is a key enzyme in the tricarboxylic acid (TCA) cycle. The TCA cycle is crucial for cellular energy production through oxidative phosphorylation and also contributes to anabolic processes. Mdh2 has significant biological importance in maintaining normal cellular metabolism and is involved in multiple physiological and pathological processes [1,2,3,4,5,6,8].
MDH2 silencing in ovarian cancer cells represses mitochondrial respiration and cell proliferation both in vitro and in vivo, indicating its role in promoting ovarian cancer malignancy [1]. In clear cell renal cell carcinoma (ccRCC), knocking out MDH2 enhances the proliferation of ccRCC cells, and MDH2 inhibits ccRCC proliferation by promoting ferroptosis [4]. In hepatocellular carcinoma (HCC), MDH2 deficiency inhibits HCC cell growth and enhances their sensitivity to ferroptosis, and MDH2 resists ferroptosis by stabilizing GPX4 [5]. In ischemic stroke, microglial lnc-U90926 binds to MDH2, affecting the decay of CXCL2 mRNA and thus neutrophil infiltration [7].
In conclusion, Mdh2 is essential for maintaining mitochondrial respiration and normal cell metabolism. Its dysregulation is closely associated with the development of various cancers such as ovarian, ccRCC, and HCC, as well as ischemic stroke. Gene knockout and knockdown models have been crucial in revealing Mdh2's role in these disease conditions, providing potential therapeutic targets for treatment [1,4,5,7].
References：
1. Pei, Xuan, Li, Kai-Yue, Shen, Yuan, Qu, Jia, Lei, Qun-Ying. 2022. Palmitoylation of MDH2 by ZDHHC18 activates mitochondrial respiration and accelerates ovarian cancer growth. In Science China. Life sciences, 65, 2017-2030. doi:10.1007/s11427-021-2048-2. https://pubmed.ncbi.nlm.nih.gov/35366151/
2. Mao, Zhifan, Liu, Wenwen, Zou, Rong, Hu, Zelan, Li, Jian. 2025. Glibenclamide targets MDH2 to relieve aging phenotypes through metabolism-regulated epigenetic modification. In Signal transduction and targeted therapy, 10, 67. doi:10.1038/s41392-025-02157-3. https://pubmed.ncbi.nlm.nih.gov/39962087/
3. She, Han, Hu, Yi, Zhao, Guozhi, Liu, Liangming, Li, Tao. 2024. Dexmedetomidine Ameliorates Myocardial Ischemia-Reperfusion Injury by Inhibiting MDH2 Lactylation via Regulating Metabolic Reprogramming. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2409499. doi:10.1002/advs.202409499. https://pubmed.ncbi.nlm.nih.gov/39467114/
4. Feng, Baijie, Su, Wei, Guo, Xianzhi, Hu, Lina, Yu, Minghua. 2024. MDH2 regulates the sensitivity of clear cell renal cell carcinoma to ferroptosis through its interaction with FSP1. In Cell death discovery, 10, 363. doi:10.1038/s41420-024-02137-6. https://pubmed.ncbi.nlm.nih.gov/39138167/
5. Yu, Wenjia, Li, Yingping, Gao, Chengchang, Deng, Qinqin, Bian, Xueli. 2024. MDH2 Promotes Hepatocellular Carcinoma Growth Through Ferroptosis Evasion via Stabilizing GPX4. In International journal of molecular sciences, 25, . doi:10.3390/ijms252111604. https://pubmed.ncbi.nlm.nih.gov/39519171/
6. Hu, Mu, Yang, JieLai, Xu, Yang, Liu, Jiao. 2022. MDH1 and MDH2 Promote Cell Viability of Primary AT2 Cells by Increasing Glucose Uptake. In Computational and mathematical methods in medicine, 2022, 2023500. doi:10.1155/2022/2023500. https://pubmed.ncbi.nlm.nih.gov/36158123/
7. Chen, Jian, Jin, Jiali, Zhang, Xi, Xia, Shengnan, Xu, Yun. 2021. Microglial lnc-U90926 facilitates neutrophil infiltration in ischemic stroke via MDH2/CXCL2 axis. In Molecular therapy : the journal of the American Society of Gene Therapy, 29, 2873-2885. doi:10.1016/j.ymthe.2021.04.025. https://pubmed.ncbi.nlm.nih.gov/33895326/
8. Li, Wei, Long, Qi, Wu, Hao, Chan, Wai-Yee, Liu, Xingguo. 2022. Nuclear localization of mitochondrial TCA cycle enzymes modulates pluripotency via histone acetylation. In Nature communications, 13, 7414. doi:10.1038/s41467-022-35199-0. https://pubmed.ncbi.nlm.nih.gov/36460681/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen