C57BL/6JCya-Furinem1flox/Cya
Common Name:
Furin-flox
Product ID:
S-CKO-04190
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Furin-flox
Strain ID
CKOCMP-18550-Furin-B6J-VA
Gene Name
Product ID
S-CKO-04190
Gene Alias
9130404I01Rik; Fur; PACE; Pcsk3; SPC1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Furinem1flox/Cya mice (Catalog S-CKO-04190) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000122232
NCBI RefSeq
NM_011046
Target Region
Exon 5~6
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
Furin, the best-characterized member of the mammalian proprotein convertases, is a ubiquitously expressed single-pass type 1 transmembrane protein. It catalyzes the proteolytic maturation of a variety of prohormones and proproteins in the secretory pathway, thus regulating the maturation of numerous proproteins and being involved in various physiological and pathophysiological processes, from embryonic development to carcinogenesis [1,2].
Furin knockout is embryonically lethal, but its knockout in differentiated somatic cells is not. Cleavage of SARS-CoV-2 spike protein by furin promotes viral entry into lung cells, making furin an exciting therapeutic target for viral pathogens like SARS-CoV-2 [1]. In cancer, while some studies propose furin as a potential target for treating several human cancers as many of its regulated proproteins are involved in cancer hallmarks, other studies show that some types of cancer do not benefit from furin inhibition [2]. In cystic fibrosis airways, elevated furin levels contribute to disease pathogenesis, and its inhibition could be a therapeutic strategy [3]. In neurodegenerative and neuropsychiatric diseases, emerging evidence like reduced FURIN mRNA expression in patients' brains implicates its role, though its role in neurological diseases is still poorly understood compared to cancer and infectious diseases [4]. In type 2 diabetes, furin's role in relation to the mTOR signaling pathway is being explored [5]. For COVID-19, targeting furin could have clinical implications as it cleaves the S1/S2 site on SARS-CoV-2 spike protein, promoting viral transmission [6].
In conclusion, Furin is essential for the proteolytic maturation of various proproteins and is involved in multiple physiological and pathophysiological processes. The knowledge from furin-related gene knockout (KO) or conditional knockout (CKO) studies, where applicable, has provided insights into its role in diseases such as viral infections, cancer, cystic fibrosis, neurodegenerative and neuropsychiatric diseases, and potentially type 2 diabetes, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Osman, Essam Eldin A, Rehemtulla, Alnawaz, Neamati, Nouri. 2021. Why All the Fury over Furin? In Journal of medicinal chemistry, 65, 2747-2784. doi:10.1021/acs.jmedchem.1c00518. https://pubmed.ncbi.nlm.nih.gov/34340303/
2. He, Zongsheng, Khatib, Abdel-Majid, Creemers, John W M. 2022. The proprotein convertase furin in cancer: more than an oncogene. In Oncogene, 41, 1252-1262. doi:10.1038/s41388-021-02175-9. https://pubmed.ncbi.nlm.nih.gov/34997216/
3. Douglas, Lisa E J, Reihill, James A, Montgomery, Bethany M, Martin, S Lorraine. 2023. Furin as a therapeutic target in cystic fibrosis airways disease. In European respiratory review : an official journal of the European Respiratory Society, 32, . doi:10.1183/16000617.0256-2022. https://pubmed.ncbi.nlm.nih.gov/37137509/
4. Zhang, Yi, Gao, Xiaoqin, Bai, Xue, Chang, Yan-Zhong, Gao, Guofen. 2022. The emerging role of furin in neurodegenerative and neuropsychiatric diseases. In Translational neurodegeneration, 11, 39. doi:10.1186/s40035-022-00313-1. https://pubmed.ncbi.nlm.nih.gov/35996194/
5. Marafie, Sulaiman K, Al-Mulla, Fahd. 2023. An Overview of the Role of Furin in Type 2 Diabetes. In Cells, 12, . doi:10.3390/cells12192407. https://pubmed.ncbi.nlm.nih.gov/37830621/
6. Jiang, Xia, Li, Dabing, Maghsoudloo, Mazaher, Ma, Wenzhe, Fu, Junjiang. 2024. Targeting furin, a cellular proprotein convertase, for COVID-19 prevention and therapeutics. In Drug discovery today, 29, 104026. doi:10.1016/j.drudis.2024.104026. https://pubmed.ncbi.nlm.nih.gov/38762086/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen