C57BL/6JCya-Ptenem1flox/Cya
Common Name:
Pten-flox
Product ID:
S-CKO-04509
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Pten-flox
Strain ID
CKOCMP-19211-Pten-B6J-VA
Gene Name
Product ID
S-CKO-04509
Gene Alias
2310035O07Rik; A130070J02Rik; B430203M17Rik; MMAC1; PTENbeta; TEP1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptenem1flox/Cya mice (Catalog S-CKO-04509) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000013807
NCBI RefSeq
NM_008960
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
PTEN, short for Phosphatase and Tensin Homolog deleted on Chromosome 10, is a crucial dual phosphatase with both protein and lipid phosphatase activities. Its lipid phosphatase activity dephosphorylates phosphatidylinositol-3,4,5-phosphate (PIP3), opposing PI3K/AKT activation. This regulation impacts numerous cellular processes like proliferation, survival, energy metabolism, cellular architecture, and motility. PTEN is widely recognized as a tumor suppressor, and its deregulation is frequently associated with cancer [1,2,4,5,6].
Conditional Pten knockout mouse models have been pivotal in exploring its role in neurodevelopment. These models show that PTEN germline mutations, while strongly associated with cancer syndromes, are also found in some autism spectrum disorder patients with macrocephaly. By studying these models, we can understand PTEN's spatiotemporal roles during neurodevelopment, such as its implications for social interactions, communication, repetitive behavior, and epilepsy [3].
In conclusion, PTEN is essential for regulating key cellular functions through its phosphatase activities and involvement in the PI3K/AKT pathway. The conditional knockout mouse models have been instrumental in revealing its roles in neurodevelopment and autism spectrum disorders, highlighting the significance of PTEN in both normal biological processes and disease conditions.
References：
1. Worby, Carolyn A, Dixon, Jack E. . PTEN. In Annual review of biochemistry, 83, 641-69. doi:10.1146/annurev-biochem-082411-113907. https://pubmed.ncbi.nlm.nih.gov/24905788/
2. Chen, Chien-Yu, Chen, Jingyu, He, Lina, Stiles, Bangyan L. 2018. PTEN: Tumor Suppressor and Metabolic Regulator. In Frontiers in endocrinology, 9, 338. doi:10.3389/fendo.2018.00338. https://pubmed.ncbi.nlm.nih.gov/30038596/
3. Rademacher, Sebastian, Eickholt, Britta J. 2019. PTEN in Autism and Neurodevelopmental Disorders. In Cold Spring Harbor perspectives in medicine, 9, . doi:10.1101/cshperspect.a036780. https://pubmed.ncbi.nlm.nih.gov/31427284/
4. Álvarez-Garcia, Virginia, Tawil, Yasmine, Wise, Helen M, Leslie, Nicholas R. 2019. Mechanisms of PTEN loss in cancer: It's all about diversity. In Seminars in cancer biology, 59, 66-79. doi:10.1016/j.semcancer.2019.02.001. https://pubmed.ncbi.nlm.nih.gov/30738865/
5. Liu, Anne, Zhu, Yanyu, Chen, Weiping, Merlino, Glenn, Yu, Yanlin. 2022. PTEN Dual Lipid- and Protein-Phosphatase Function in Tumor Progression. In Cancers, 14, . doi:10.3390/cancers14153666. https://pubmed.ncbi.nlm.nih.gov/35954330/
6. Langdon, Casey G. 2023. Nuclear PTEN's Functions in Suppressing Tumorigenesis: Implications for Rare Cancers. In Biomolecules, 13, . doi:10.3390/biom13020259. https://pubmed.ncbi.nlm.nih.gov/36830628/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen