C57BL/6JCya-Ptprkem1flox/Cya
Common Name:
Ptprk-flox
Product ID:
S-CKO-04604
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ptprk-flox
Strain ID
CKOCMP-19272-Ptprk-B6J-VA
Gene Name
Product ID
S-CKO-04604
Gene Alias
PTPk; RPTPkappa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ptprkem1flox/Cya mice (Catalog S-CKO-04604) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000166468
NCBI RefSeq
NM_008983
Target Region
Exon 3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ptprk, Protein Tyrosine Phosphatase Receptor Type K, is a receptor tyrosine phosphatase. It is involved in regulating growth factor signalling, cell-cell adhesion, and acts as a potential tumour suppressor. It is associated with multiple pathways such as Wnt signalling and is important in various biological processes like cell proliferation, migration, and differentiation [2,3,4,5]. Genetic models, especially KO mouse models, are valuable in studying its functions.
In obesity-related studies, high-fat-fed PTPRK knockout male and female mice have lower weight gain and reduced hepatic fat accumulation. PTPRK was found to regulate glycolysis and de novo lipogenesis in hepatocytes, with its induced glycolysis enhancing PPARγ and lipogenesis [1]. In colorectal cancer, PTPRK knockout mouse models and xenografts confirm its role as a tumour suppressor, regulating growth factor and adhesion signalling, and suppressing epithelial to mesenchymal transition (EMT) in a catalysis-independent manner [2]. In colon cancer cells, knockdown of PTPRK potentiates the pro-oncogenic CD133-AKT pathway, reducing sensitivity to oxaliplatin [3].
In conclusion, Ptprk plays essential roles in metabolic regulation, intestinal repair, and tumour suppression. The use of Ptprk KO mouse models has provided valuable insights into its functions in obesity-associated metabolic diseases and cancer, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Gilglioni, Eduardo H, Li, Ao, St-Pierre-Wijckmans, Wadsen, Wu, Wei, Gurzov, Esteban N. 2024. PTPRK regulates glycolysis and de novo lipogenesis to promote hepatocyte metabolic reprogramming in obesity. In Nature communications, 15, 9522. doi:10.1038/s41467-024-53733-0. https://pubmed.ncbi.nlm.nih.gov/39496584/
2. Young, Katherine A, Wojdyla, Katarzyna, Lai, Tiffany, Fearnley, Gareth W, Sharpe, Hayley J. 2024. The receptor protein tyrosine phosphatase PTPRK promotes intestinal repair and catalysis-independent tumour suppression. In Journal of cell science, 137, . doi:10.1242/jcs.261914. https://pubmed.ncbi.nlm.nih.gov/38904097/
3. Matsushita, Masashi, Mori, Yusuke, Uchiumi, Kyosuke, Shimozato, Osamu, Ozaki, Toshinori. 2019. PTPRK suppresses progression and chemo-resistance of colon cancer cells via direct inhibition of pro-oncogenic CD133. In FEBS open bio, 9, 935-946. doi:10.1002/2211-5463.12636. https://pubmed.ncbi.nlm.nih.gov/30947381/
4. Kim, Minseong, Reinhard, Carmen, Niehrs, Christof. 2021. A MET-PTPRK kinase-phosphatase rheostat controls ZNRF3 and Wnt signaling. In eLife, 10, . doi:10.7554/eLife.70885. https://pubmed.ncbi.nlm.nih.gov/34590584/
5. Chang, Ling-Shih, Kim, Minseong, Glinka, Andrey, Reinhard, Carmen, Niehrs, Christof. 2020. The tumor suppressor PTPRK promotes ZNRF3 internalization and is required for Wnt inhibition in the Spemann organizer. In eLife, 9, . doi:10.7554/eLife.51248. https://pubmed.ncbi.nlm.nih.gov/31934854/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen