C57BL/6JCya-Rpiaem1flox/Cya
Common Name:
Rpia-flox
Product ID:
S-CKO-04833
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rpia-flox
Strain ID
CKOCMP-19895-Rpia-B6J-VA
Gene Name
Product ID
S-CKO-04833
Gene Alias
RPI
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rpiaem1flox/Cya mice (Catalog S-CKO-04833) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066134
NCBI RefSeq
NM_009075
Target Region
Exon 2
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
RPIA, ribose 5-phosphate isomerase-A, is an enzyme-encoding gene. It is a key enzyme in the pentose phosphate pathway, which is crucial for producing NADPH and pentose precursors for nucleotides. This pathway runs parallel to central glycolysis. The gene plays a significant role in multiple biological processes, including cell proliferation, metabolism, and in maintaining redox control [2,3,4,5,6].
Mutations in RPIA have been associated with a rare genetic leukoencephalopathy [1]. In Enterobacter cloacae, mutation of rpiA decreased seed and root colonization and biocontrol of damping-off caused by Pythium ultimum on cucumber, indicating its role in plant-microbe interactions [4]. In human cancer cells, targeting RPIA can impact cell proliferation. For example, in colorectal cancer cells, miR124 reduces the pentose phosphate pathway and proliferation by targeting RPIA mRNA. Also, in endometrial cancer cells, knockdown of RPIA reduces cell proliferation, accompanied by decreased glycolytic capacity and oxidative phosphorylation [2,3]. In macrophages, the antiviral effector PKR phosphorylates and inhibits RPIA, suppressing nucleic acid production during the immune response to RNA [6].
In conclusion, RPIA is essential for the pentose phosphate pathway and has far-reaching impacts on various biological processes. Studies on RPIA-related loss-of-function in different organisms and cell types, including in cancer and in response to infection, have revealed its significance in disease-related processes, providing potential targets for treatment strategies.
References:
1. Kaur, Parneet, Wamelink, Mirjam M C, van der Knaap, Marjo S, Girisha, Katta Mohan, Shukla, Anju. 2019. Confirmation of a Rare Genetic Leukoencephalopathy due to a Novel Bi-allelic Variant in RPIA. In European journal of medical genetics, 62, 103708. doi:10.1016/j.ejmg.2019.103708. https://pubmed.ncbi.nlm.nih.gov/31247379/
2. Qiu, Zhaoping, Guo, Weijie, Wang, Qifeng, Zhao, Yingjun, He, Xianghuo. 2015. MicroRNA-124 reduces the pentose phosphate pathway and proliferation by targeting PRPS1 and RPIA mRNAs in human colorectal cancer cells. In Gastroenterology, 149, 1587-1598.e11. doi:10.1053/j.gastro.2015.07.050. https://pubmed.ncbi.nlm.nih.gov/26248089/
3. Szwarc, Maria M, Kommagani, Ramakrishna, Putluri, Vasanta, Lydon, John P, O'Malley, Bert W. 2018. Steroid Receptor Coactivator-2 Controls the Pentose Phosphate Pathway through RPIA in Human Endometrial Cancer Cells. In Scientific reports, 8, 13134. doi:10.1038/s41598-018-31372-y. https://pubmed.ncbi.nlm.nih.gov/30177747/
4. Lohrke, Scott M, Dery, Pierre D, Li, Wei, Kobayashi, Donald Y, Roberts, Daniel R. . Mutation of rpiA in Enterobacter cloacae decreases seed and root colonization and biocontrol of damping-off caused by Pythium ultimum on cucumber. In Molecular plant-microbe interactions : MPMI, 15, 817-25. doi:. https://pubmed.ncbi.nlm.nih.gov/12182339/
5. Hove-Jensen, B, Maigaard, M. . Escherichia coli rpiA gene encoding ribose phosphate isomerase A. In Journal of bacteriology, 175, 5628-35. doi:. https://pubmed.ncbi.nlm.nih.gov/8366047/
6. Bhattacharjee, Pushpak, Wang, Die, Anderson, Dovile, Harris, Lawrence D, Sadler, Anthony J. 2024. The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate. In The EMBO journal, 43, 2636-2660. doi:10.1038/s44318-024-00100-w. https://pubmed.ncbi.nlm.nih.gov/38778156/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen