C57BL/6NCya-Scd1em1flox/Cya
Common Name:
Scd1-flox
Product ID:
S-CKO-04923
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Scd1-flox
Strain ID
CKOCMP-20249-Scd1-B6N-VA
Gene Name
Product ID
S-CKO-04923
Gene Alias
Scd; Scd-1; ab
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Scd1em1flox/Cya mice (Catalog S-CKO-04923) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041331
NCBI RefSeq
NM_009127
Target Region
Exon 3
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Scd1, or stearoyl-CoA desaturase 1, is a key enzyme in lipid metabolism. It desaturates saturated fatty acids (SFAs) derived from de novo lipogenesis or diet to generate monounsaturated fatty acids (MUFAs). SCD1 is involved in multiple pathways such as AMPK/ACC, SIRT1/PGC1α, and NcDase/Wnt, and is crucial for maintaining metabolic and tissue homeostasis, regulating processes like energy homeostasis, development, autophagy, tumorigenesis, and inflammation [6].
In cancer, SCD1 promotes cell proliferation and metastasis. For example, in gastric cancer, ADAR1-mediated RNA editing of SCD1 increases its mRNA stability, facilitating lipid droplet formation to alleviate chemotherapy-induced ER stress and enhancing self-renewal [3]. In ovarian cancer, SCD1 is aberrantly upregulated in ascites-derived cells, accelerating lipid metabolic activities and tumor aggressiveness, and its inhibition retards tumor growth [1]. In hepatocellular carcinoma, an SCD1-dependent mechanoresponsive pathway promotes invasion and metastasis through lipid metabolic reprogramming in response to matrix stiffness [5]. In colorectal cancer, TIGAR drives ferroptosis resistance via the ROS/AMPK/SCD1 pathway [8], and aspirin promotes RSL3-induced ferroptosis by suppressing mTOR/SREBP-1/SCD1-mediated lipogenesis in PIK3CA-mutant cells [7]. Also, in triple-negative breast cancer, salidroside sensitizes cells to ferroptosis by inhibiting SCD1-mediated lipogenesis [4]. In non-alcoholic fatty liver disease, berberine reduces liver triglyceride synthesis and attenuates hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway [2]. Moreover, inhibition of SCD1 enhances the antitumor T cell response and synergizes with anti-PD-1 antibody [9].
In conclusion, Scd1 plays essential roles in lipid metabolism and is intricately involved in various disease conditions, especially cancer and metabolic diseases. Studies using gene-knockout or conditional-knockout mouse models (although not explicitly detailed in the provided abstracts but inferred from the general research context) would be valuable to further understand its functions in these diseases, potentially providing new therapeutic targets for treatment.
References:
1. Xuan, Yang, Wang, Huogang, Yung, Mingo Mh, Chan, Karen Kl, Chan, David W. 2022. SCD1/FADS2 fatty acid desaturases equipoise lipid metabolic activity and redox-driven ferroptosis in ascites-derived ovarian cancer cells. In Theranostics, 12, 3534-3552. doi:10.7150/thno.70194. https://pubmed.ncbi.nlm.nih.gov/35547771/
2. Zhu, Xiaopeng, Bian, Hua, Wang, Liu, Li, Xiaoying, Gao, Xin. 2019. Berberine attenuates nonalcoholic hepatic steatosis through the AMPK-SREBP-1c-SCD1 pathway. In Free radical biology & medicine, 141, 192-204. doi:10.1016/j.freeradbiomed.2019.06.019. https://pubmed.ncbi.nlm.nih.gov/31226399/
3. Wong, Tin-Lok, Loh, Jia-Jian, Lu, Shixun, Leung, Suet Yi, Ma, Stephanie. 2023. ADAR1-mediated RNA editing of SCD1 drives drug resistance and self-renewal in gastric cancer. In Nature communications, 14, 2861. doi:10.1038/s41467-023-38581-8. https://pubmed.ncbi.nlm.nih.gov/37208334/
4. Huang, Guiqin, Cai, Yawen, Ren, Menghui, Zhu, Lingpeng, Yan, Tianhua. 2024. Salidroside sensitizes Triple-negative breast cancer to ferroptosis by SCD1-mediated lipogenesis and NCOA4-mediated ferritinophagy. In Journal of advanced research, , . doi:10.1016/j.jare.2024.09.027. https://pubmed.ncbi.nlm.nih.gov/39353532/
5. Liu, Hua-Hua, Xu, Yang, Li, Cao-Jie, Ren, Zheng-Gang, Chen, Rong-Xin. 2022. An SCD1-dependent mechanoresponsive pathway promotes HCC invasion and metastasis through lipid metabolic reprogramming. In Molecular therapy : the journal of the American Society of Gene Therapy, 30, 2554-2567. doi:10.1016/j.ymthe.2022.03.015. https://pubmed.ncbi.nlm.nih.gov/35358687/
6. Sun, Qin, Xing, Xiaorui, Wang, Huanyu, Wang, Yibing, Wang, Ru. 2023. SCD1 is the critical signaling hub to mediate metabolic diseases: Mechanism and the development of its inhibitors. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 170, 115586. doi:10.1016/j.biopha.2023.115586. https://pubmed.ncbi.nlm.nih.gov/38042113/
7. Chen, Hao, Qi, Qinqin, Wu, Nan, Jin, Rong, Jiang, Lei. 2022. Aspirin promotes RSL3-induced ferroptosis by suppressing mTOR/SREBP-1/SCD1-mediated lipogenesis in PIK3CA-mutant colorectal cancer. In Redox biology, 55, 102426. doi:10.1016/j.redox.2022.102426. https://pubmed.ncbi.nlm.nih.gov/35963119/
8. Liu, Min-Yao, Li, Hong-Ming, Wang, Xin-Yu, Wang, Miao, Zhang, Hong-Sheng. 2022. TIGAR drives colorectal cancer ferroptosis resistance through ROS/AMPK/SCD1 pathway. In Free radical biology & medicine, 182, 219-231. doi:10.1016/j.freeradbiomed.2022.03.002. https://pubmed.ncbi.nlm.nih.gov/35271998/
9. Katoh, Yuki, Yaguchi, Tomonori, Kubo, Akiko, Suematsu, Makoto, Kawakami, Yutaka. . Inhibition of stearoyl-CoA desaturase 1 (SCD1) enhances the antitumor T cell response through regulating β-catenin signaling in cancer cells and ER stress in T cells and synergizes with anti-PD-1 antibody. In Journal for immunotherapy of cancer, 10, . doi:10.1136/jitc-2022-004616. https://pubmed.ncbi.nlm.nih.gov/35793868/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen