C57BL/6JCya-Slit2em1flox/Cya
Common Name:
Slit2-flox
Product ID:
S-CKO-05125
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Slit2-flox
Strain ID
CKOCMP-20563-Slit2-B6J-VB
Gene Name
Product ID
S-CKO-05125
Gene Alias
Drad-1; E030015M03Rik; E130320P19Rik; Slil3; b2b1200.1Clo; mKIAA4141; slit-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slit2em1flox/Cya mice (Catalog S-CKO-05125) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000174421
NCBI RefSeq
NM_001291227
Target Region
Exon 8
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Slit2 is a secreted polypeptide that was initially known for its role in regulating the migration of developing axons during central nervous system (CNS) development, acting through Roundabout (Robo) receptors to mediate intracellular signal transduction pathways [4]. It has since been found to be involved in various biological processes such as cell migration, immune response, vascular permeability, and angiogenesis [4].
In cancer, its role seems context-dependent. Deleting endothelial Slit2 suppressed metastatic dissemination in mouse models of breast and lung cancer, while deletion of tumoural Slit2 enhanced metastatic progression, suggesting that cancer cells co-opt innate RNA sensing to induce a chemotactic signalling pathway in endothelium via the TLR3-SLIT2 axis [1]. In gliomas, knockdown of SLIT2 in mouse glioma cells and patient-derived GBM xenografts reduced tumor growth and rendered tumors sensitive to immunotherapy [2]. In Ewing sarcoma, silencing of Slit2 strongly inhibited the growth of Ewing sarcoma cells [5]. Conversely, in small-cell lung cancer, SLIT2 overexpression restricted tumor growth in vitro and in vivo, and in gastric cancer, exosomal miR-4745-5p/3911 from N2-polarized tumor-associated neutrophils promoted metastasis by downregulating SLIT2 [6,7].
In liver fibrosis, Slit2-Robo1 signaling promoted liver injury and fibrosis through activation of hepatic stellate cells, as Slit2-Tg mice were more vulnerable to CCl4-induced liver injury and fibrosis while Robo1/2(+/-) mice had attenuated responses [3].
In conclusion, Slit2 plays diverse and context-specific roles in various biological processes and diseases. Gene knockout (KO) and conditional knockout (CKO) mouse models have been instrumental in revealing its role in cancer metastasis, glioma growth, liver fibrosis, among others. Understanding the functions of Slit2 provides potential therapeutic targets for these diseases.
References:
1. Tavora, Bernardo, Mederer, Tobias, Wessel, Kai J, Goodarzi, Hani, Tavazoie, Sohail F. 2020. Tumoural activation of TLR3-SLIT2 axis in endothelium drives metastasis. In Nature, 586, 299-304. doi:10.1038/s41586-020-2774-y. https://pubmed.ncbi.nlm.nih.gov/32999457/
2. Geraldo, Luiz H, Xu, Yunling, Jacob, Laurent, Eichmann, Anne, Mathivet, Thomas. . SLIT2/ROBO signaling in tumor-associated microglia and macrophages drives glioblastoma immunosuppression and vascular dysmorphia. In The Journal of clinical investigation, 131, . doi:10.1172/JCI141083. https://pubmed.ncbi.nlm.nih.gov/34181595/
3. Chang, Jianlan, Lan, Tian, Li, Changzheng, Ding, Yanqing, Wang, Lijing. 2015. Activation of Slit2-Robo1 signaling promotes liver fibrosis. In Journal of hepatology, 63, 1413-20. doi:10.1016/j.jhep.2015.07.033. https://pubmed.ncbi.nlm.nih.gov/26264936/
4. Sherchan, Prativa, Travis, Zachary D, Tang, Jiping, Zhang, John H. 2020. The potential of Slit2 as a therapeutic target for central nervous system disorders. In Expert opinion on therapeutic targets, 24, 805-818. doi:10.1080/14728222.2020.1766445. https://pubmed.ncbi.nlm.nih.gov/32378435/
5. Suvarna, Kruthi, Jayabal, Panneerselvam, Ma, Xiuye, Shiio, Yuzuru. 2022. Slit2 signaling stimulates Ewing sarcoma growth. In Genes & cancer, 13, 88-99. doi:10.18632/genesandcancer.227. https://pubmed.ncbi.nlm.nih.gov/36533189/
6. Ahirwar, Dinesh K, Peng, Bo, Charan, Manish, Miles, Wayne O, Ganju, Ramesh K. 2023. Slit2/Robo1 signaling inhibits small-cell lung cancer by targeting β-catenin signaling in tumor cells and macrophages. In Molecular oncology, 17, 839-856. doi:10.1002/1878-0261.13289. https://pubmed.ncbi.nlm.nih.gov/35838343/
7. Zhang, Jiahui, Yu, Dan, Ji, Cheng, Gu, Jianmei, Zhang, Xu. 2024. Exosomal miR-4745-5p/3911 from N2-polarized tumor-associated neutrophils promotes gastric cancer metastasis by regulating SLIT2. In Molecular cancer, 23, 198. doi:10.1186/s12943-024-02116-6. https://pubmed.ncbi.nlm.nih.gov/39272149/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen