C57BL/6NCya-Kdm5cem1flox/Cya
Common Name:
Kdm5c-flox
Product ID:
S-CKO-05134
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Kdm5c-flox
Strain ID
CKOCMP-20591-Kdm5c-B6N-VA
Gene Name
Product ID
S-CKO-05134
Gene Alias
D930009K15Rik; Jarid1c; Smcx; mKIAA0234
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Kdm5cem1flox/Cya mice (Catalog S-CKO-05134) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112588
NCBI RefSeq
NM_013668
Target Region
Exon 18
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
Kdm5c, also known as JARID1C, is a histone H3K4 demethylase. It removes di-and tri-methyl groups of lysine 4 on histone H3 from transcriptional targets, playing a crucial role in regulating transcription by altering chromatin. It is involved in various biological processes and is essential for neuronal survival, dendritic growth, and normal development [1,4,6].
In Kdm5c knockout mice, disruption of Kdm5c leads to intellectual disability as it fails to ensure neurodevelopment occurs at an appropriate timescale. Specifically, it controls WNT output during a specific developmental window, regulating the transition of primary to intermediate progenitor cells and neurogenesis. WNT inhibition during this period can rescue the behavioral changes in knockout mice [2].
In female mice, loss of Kdm5c in hematopoietic stem cells or bone marrow monocytes increases bone mass due to impaired osteoclastogenesis and bioenergetic metabolism, while this effect is not seen in males [3].
In acute myeloid leukemia (AML) mouse models, reduced Kdm5C expression accelerates growth, indicating its role as a tumor suppressor. Kdm5C knockdown leads to increased H3K4me3 levels and up-regulation of bivalently marked immature genes [5].
In conclusion, Kdm5c is vital for normal neurodevelopment, bone mass regulation in females, and acts as a tumor suppressor in AML. Studies using Kdm5c knockout mouse models have significantly contributed to understanding its functions in these biological processes and disease conditions, providing insights into potential therapeutic targets for related disorders.
References：
1. Chang, Soojeong, Yim, Sujin, Park, Hyunsung. 2019. The cancer driver genes IDH1/2, JARID1C/ KDM5C, and UTX/ KDM6A: crosstalk between histone demethylation and hypoxic reprogramming in cancer metabolism. In Experimental & molecular medicine, 51, 1-17. doi:10.1038/s12276-019-0230-6. https://pubmed.ncbi.nlm.nih.gov/31221981/
2. Karwacki-Neisius, Violetta, Jang, Ahram, Cukuroglu, Engin, Pomeroy, Scott L, Shi, Yang. 2024. WNT signalling control by KDM5C during development affects cognition. In Nature, 627, 594-603. doi:10.1038/s41586-024-07067-y. https://pubmed.ncbi.nlm.nih.gov/38383780/
3. Liu, Huadie, Zhai, Lukai, Liu, Ye, Yang, Tao, Krawczyk, Connie M. 2023. The histone demethylase KDM5C controls female bone mass by promoting energy metabolism in osteoclasts. In Science advances, 9, eadg0731. doi:10.1126/sciadv.adg0731. https://pubmed.ncbi.nlm.nih.gov/37018401/
4. Gonçalves, Thainá Fernandez, Gonçalves, Andressa Pereira, Fintelman Rodrigues, Natalia, Pimentel, Márcia Mattos Gonçalves, Santos-Rebouças, Cíntia Barros. 2014. KDM5C mutational screening among males with intellectual disability suggestive of X-Linked inheritance and review of the literature. In European journal of medical genetics, 57, 138-44. doi:10.1016/j.ejmg.2014.02.011. https://pubmed.ncbi.nlm.nih.gov/24583395/
5. Trempenau, Mette Louise, Schuster, Mikkel Bruhn, Pundhir, Sachin, Helin, Kristian, Porse, Bo T. 2023. The histone demethylase KDM5C functions as a tumor suppressor in AML by repression of bivalently marked immature genes. In Leukemia, 37, 593-605. doi:10.1038/s41375-023-01810-6. https://pubmed.ncbi.nlm.nih.gov/36631623/
6. Hatch, Hayden A M, Secombe, Julie. 2021. Molecular and cellular events linking variants in the histone demethylase KDM5C to the intellectual disability disorder Claes-Jensen syndrome. In The FEBS journal, 289, 7776-7787. doi:10.1111/febs.16204. https://pubmed.ncbi.nlm.nih.gov/34536985/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen