C57BL/6JCya-Serpina3nem1flox/Cya
Common Name:
Serpina3n-flox
Product ID:
S-CKO-05204
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Serpina3n-flox
Strain ID
CKOCMP-20716-Serpina3n-B6J-VA
Gene Name
Product ID
S-CKO-05204
Gene Alias
Spi2-2; Spi2.2; Spi2/eb.4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Serpina3nem1flox/Cya mice (Catalog S-CKO-05204) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021506
NCBI RefSeq
NM_009252
Target Region
Exon 3~5
Size of Effective Region
~2.9 kb
Detailed Document
Overview of Gene Research
Serpina3n, a secretory serine protease inhibitor belonging to clade "a", exhibits unique structural and physiological characteristics. It plays significant roles in various biological processes such as the complement cascade, apoptosis, and wound healing by inhibiting a wide range of proteases [2]. Clinically, its human orthologue SERPINA3 is dysregulated in brain cells, cerebrospinal fluid, and plasma in various neurological conditions [1].
In a Serpina3n conditional knockout mice model, genetic deletion of SerpinA3n led to increased activity of substrate proteases, poorly compacted matrix, and significantly worse post-infarct cardiac function. This demonstrated a causal role of Serpins in regulating matrix function and post-infarct cardiac remodelling [3]. In another study, hepatic deletion of SerpinA3N in male mice attenuated steatosis, correlated with altered lipid metabolism genes, increased fatty acid oxidation activity, and enhanced insulin signaling in mice with non-alcoholic fatty liver disease (NAFLD) [4].
In conclusion, Serpina3n is involved in multiple biological processes, and its functions are further elucidated through gene knockout mouse models. These models have revealed its roles in cardiac remodelling after myocardial infarction and in regulating NAFLD and glucose homeostasis, providing insights into potential therapeutic strategies for related diseases.
References:
1. Zhu, Meina, Lan, Zhaohui, Park, Joohyun, Wang, Yan, Guo, Fuzheng. . Regulation of CNS pathology by Serpina3n/SERPINA3: The knowns and the puzzles. In Neuropathology and applied neurobiology, 50, e12980. doi:10.1111/nan.12980. https://pubmed.ncbi.nlm.nih.gov/38647003/
2. Aslam, Mehwish Saba, Yuan, Liudi. 2019. Serpina3n: Potential drug and challenges, mini review. In Journal of drug targeting, 28, 368-378. doi:10.1080/1061186X.2019.1693576. https://pubmed.ncbi.nlm.nih.gov/31790278/
3. Sun, Qihao, Chen, Wei, Wu, Rimao, Li, Shen, Deb, Arjun. . Serine protease inhibitor, SerpinA3n, regulates cardiac remodelling after myocardial infarction. In Cardiovascular research, 120, 943-953. doi:10.1093/cvr/cvae075. https://pubmed.ncbi.nlm.nih.gov/38666458/
4. Tran, Melanie, Mostofa, Golam, Picard, Michael, Wang, Li, Shin, Dong-Ju. 2023. SerpinA3N deficiency attenuates steatosis and enhances insulin signaling in male mice. In The Journal of endocrinology, 256, . doi:10.1530/JOE-22-0073. https://pubmed.ncbi.nlm.nih.gov/36625462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen