C57BL/6JCya-Tap1em1flox/Cya
Common Name:
Tap1-flox
Product ID:
S-CKO-05736
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tap1-flox
Strain ID
CKOCMP-21354-Tap1-B6J-VA
Gene Name
Product ID
S-CKO-05736
Gene Alias
ABC17; APT1; Abcb2; Ham-1; Ham1; MTP1; PSF1; RING4; TAP; Tap-1; Y3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tap1em1flox/Cya mice (Catalog S-CKO-05736) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000170086
NCBI RefSeq
NM_013683
Target Region
Exon 4~5
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Tap1, also known as transporter associated with antigen processing 1, is a member of the ATP-binding cassette (ABC) family. It is crucial for transporting antigen peptides from the cytoplasm to the lumen of the endoplasmic reticulum and then loading them onto major histocompatibility complex (MHC) class I molecules, thus playing a significant role in the immune response, especially in antigen presentation to regulate adaptive immunity [6,9]. It is also involved in interferon-stimulated gene (ISG)-related pathways as it has been shown to promote interferon-β production [9].
Tap1 has been studied in various disease contexts. In ankylosing spondylitis, certain polymorphisms in Tap1 (Tap1-333Val, Tap1-637Gly) were associated with the disease, especially when compared with HLA-B27-negative controls [1]. In uveal melanoma, upregulated Tap1 was linked to shorter survival, higher metastasis likelihood, and higher mortality. In vitro silencing of Tap1 in C918 cells inhibited cell proliferation and metastasis, suggesting its potential as an oncogene and prognostic marker [2]. In gastric cancer, Tap1 was identified as a T-cell related therapeutic target, and oxaliplatin could enhance immunotherapy by promoting Tap1 expression [3]. In pancreatic cancer, Tap1 promoted resistance to MEK inhibitors by enhancing drug transport out of cells, and its suppression could sensitize cells to the inhibitor [4]. In colorectal cancer, down-regulation of Tap1 was associated with immune escape and poor prognosis, and methylation of the Tap1 gene may be a mechanism for this down-regulation [5]. In gastric carcinoma, Tap1 was associated with an inflamed tumor microenvironment and could be a biomarker for immunotherapeutic response [6]. In pan-cancer analysis, Tap1 was a tumor prognostic biomarker and a predictor of immunotherapeutic responses [7]. In locally advanced gastric cancer, high Tap1 expression was associated with better overall survival [8]. Also, Tap1 has broadly antiviral activities through activating the TBK1-IRF3-mediated type I interferon production [9].
In conclusion, Tap1 is essential for antigen presentation and immune-related processes. Studies in various disease models have revealed its diverse roles in disease development, prognosis, and response to therapy. These findings highlight the importance of Tap1 in understanding disease mechanisms and developing potential therapeutic strategies for diseases such as ankylosing spondylitis, different types of cancers, and viral infections.
References:
1. Qian, Yufeng, Wang, Genlin, Xue, Feng, Tang, Liang, Yang, Huilin. 2017. Genetic association between TAP1 and TAP2 polymorphisms and ankylosing spondylitis: a systematic review and meta-analysis. In Inflammation research : official journal of the European Histamine Research Society ... [et al.], 66, 653-661. doi:10.1007/s00011-017-1047-1. https://pubmed.ncbi.nlm.nih.gov/28405734/
2. Zhu, Ru, Chen, Yu-Ting, Wang, Bo-Wen, Jiang, Fa-Gang, Zhang, Ming-Chang. 2023. TAP1, a potential immune-related prognosis biomarker with functional significance in uveal melanoma. In BMC cancer, 23, 146. doi:10.1186/s12885-023-10527-9. https://pubmed.ncbi.nlm.nih.gov/36774490/
3. Zhao, Yupeng, Liu, Ziyuan, Deng, Kaiyuan, Li, Ranran, Xia, Jiazeng. 2024. Identification of TAP1 as a T-cell related therapeutic target in gastric cancer by mediating oxalipliatin-related synergistic enhancement of immunotherapy. In International immunopharmacology, 132, 111998. doi:10.1016/j.intimp.2024.111998. https://pubmed.ncbi.nlm.nih.gov/38593510/
4. Li, Boya, Feng, Yu, Hou, Qiaoyun, Fu, Yan, Luo, Yongzhang. 2022. Antigen Peptide Transporter 1 (TAP1) Promotes Resistance to MEK Inhibitors in Pancreatic Cancers. In International journal of molecular sciences, 23, . doi:10.3390/ijms23137168. https://pubmed.ncbi.nlm.nih.gov/35806187/
5. Ling, Agnes, Löfgren-Burström, Anna, Larsson, Pär, Edin, Sofia, Palmqvist, Richard. 2017. TAP1 down-regulation elicits immune escape and poor prognosis in colorectal cancer. In Oncoimmunology, 6, e1356143. doi:10.1080/2162402X.2017.1356143. https://pubmed.ncbi.nlm.nih.gov/29147604/
6. He, Zehua, Yang, Hong, Chen, Qingfeng, He, Wanrong, Chen, Zhihui. 2024. Role of TAP1 in the identification of immune-hot tumor microenvironment and its prognostic significance for immunotherapeutic efficacy in gastric carcinoma. In Journal of gastrointestinal oncology, 15, 890-907. doi:10.21037/jgo-24-28. https://pubmed.ncbi.nlm.nih.gov/38989426/
7. Tu, Zewei, Li, Kuangxun, Ji, Qiankun, Huang, Kai, Zhu, Xingen. 2023. Pan-cancer analysis: predictive role of TAP1 in cancer prognosis and response to immunotherapy. In BMC cancer, 23, 133. doi:10.1186/s12885-022-10491-w. https://pubmed.ncbi.nlm.nih.gov/36759763/
8. Segami, Kenki, Aoyama, Toru, Hiroshima, Yukihiko, Saeki, Hiroshi, Oshima, Takashi. . Clinical Significance of TAP1 and DLL4 Expression in Patients With Locally Advanced Gastric Cancer. In In vivo (Athens, Greece), 35, 2771-2777. doi:10.21873/invivo.12562. https://pubmed.ncbi.nlm.nih.gov/34410967/
9. Zhao, Jin, Li, Ruiting, Li, Yanjun, Feng, Fengling, Sun, Caijun. 2021. Broadly Antiviral Activities of TAP1 through Activating the TBK1-IRF3-Mediated Type I Interferon Production. In International journal of molecular sciences, 22, . doi:10.3390/ijms22094668. https://pubmed.ncbi.nlm.nih.gov/33925089/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen