C57BL/6JCya-Arhgap9em1flox/Cya
Common Name
Arhgap9-flox
Product ID
S-CKO-05996
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-216445-Arhgap9-B6J-VA
When using this mouse strain in a publication, please cite “Arhgap9-flox Mouse (Catalog S-CKO-05996) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Arhgap9-flox
Strain ID
CKOCMP-216445-Arhgap9-B6J-VA
Gene Name
Product ID
S-CKO-05996
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 10
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000219511
NCBI RefSeq
NM_001285785
Target Region
Exon 3~10
Size of Effective Region
~3.0 kb
Overview of Gene Research
Arhgap9, also known as Rho GTPase activating protein 9, is a gene that can inactivate Rho GTPases by hydrolyzing GTP into GDP. It is involved in regulating multiple cancer-related cellular events through inhibiting JNK/ERK/p38 and PI3K/AKT signaling pathways, and its genetic/epigenetic variations and abnormal expression are associated with various diseases, especially cancers [1].
In lung adenocarcinoma, knockdown of Arhgap9 promotes cell proliferation, migration, and invasion, inhibits apoptosis, and reduces G0G1 cell cycle arrest by activating the Wnt/β-catenin signaling pathway via suppressing DKK2 [2]. In colorectal cancer, over-expression of Arhgap9 inhibits cell proliferation, invasion, migration, and epithelial-mesenchymal transition (EMT), while knockdown promotes these processes. Arhgap9 exerts these effects by targeting the PI3K/AKT/mTOR signaling pathway [3]. In hepatocellular carcinoma, Arhgap9 overexpression can inhibit cell proliferation, migration, invasion, and lung metastases through up-regulating FOXJ2/E-cadherin [4]. In gastric cancer, Arhgap9 siRNA inhibits cell viability, migration, invasion, and EMT via inactivating Akt and p38 signaling and inhibiting MMP2 and MMP9 [5]. In bladder cancer, down-regulation of Arhgap9 expression is associated with a poor prognosis [6]. In breast cancer, knockdown of Arhgap9 reduces cell proliferation, migration, and invasive ability, and induces G0-G1 cell cycle arrest and apoptosis [7].
In conclusion, Arhgap9 plays a crucial role in regulating cancer-related cellular processes such as proliferation, apoptosis, migration, invasion, and EMT. Through various loss-of-function experiments in different cancer cell lines, its significance in multiple cancer types has been revealed, highlighting its potential as a biomarker and therapeutic target for cancer treatment.
References：
1. Song, Wenping, Chen, Jinhua, Li, Shuolei, Zhang, Wenzhou, Li, Liang. . Rho GTPase Activating Protein 9 (ARHGAP9) in Human Cancers. In Recent patents on anti-cancer drug discovery, 17, 55-65. doi:10.2174/1574892816666210806155754. https://pubmed.ncbi.nlm.nih.gov/34365932/
2. Song, Wenping, Wu, Xuan, Cheng, Cheng, Chen, Jinhua, Zhang, Wenzhou. 2023. ARHGAP9 knockdown promotes lung adenocarcinoma metastasis by activating Wnt/β-catenin signaling pathway via suppressing DKK2. In Genomics, 115, 110684. doi:10.1016/j.ygeno.2023.110684. https://pubmed.ncbi.nlm.nih.gov/37454937/
3. Sun, Jufeng, Zhao, Xiaoguang, Jiang, Huamao, Ma, Yinda, Qian, Zhiyu. 2022. ARHGAP9 inhibits colorectal cancer cell proliferation, invasion and EMT via targeting PI3K/AKT/mTOR signaling pathway. In Tissue & cell, 77, 101817. doi:10.1016/j.tice.2022.101817. https://pubmed.ncbi.nlm.nih.gov/35679685/
4. Zhang, Hong, Tang, Qing-Feng, Sun, Meng-Yao, Zhang, Li-Jun, Zhang, Hong. 2018. ARHGAP9 suppresses the migration and invasion of hepatocellular carcinoma cells through up-regulating FOXJ2/E-cadherin. In Cell death & disease, 9, 916. doi:10.1038/s41419-018-0976-0. https://pubmed.ncbi.nlm.nih.gov/30206221/
5. Sun, Lingjia, Zhang, Youping, Lou, Jie. 2017. ARHGAP9 siRNA inhibits gastric cancer cell proliferation and EMT via inactivating Akt, p38 signaling and inhibiting MMP2 and MMP9. In International journal of clinical and experimental pathology, 10, 11979-11985. doi:. https://pubmed.ncbi.nlm.nih.gov/31966562/
6. Piao, Xuan-Mei, Jeong, Pildu, Yan, Chunri, Yun, Seok Joong, Kim, Wun-Jae. 2019. A novel tumor suppressing gene, ARHGAP9, is an independent prognostic biomarker for bladder cancer. In Oncology letters, 19, 476-486. doi:10.3892/ol.2019.11123. https://pubmed.ncbi.nlm.nih.gov/31897161/
7. Wang, Tianyi, Ha, Minwen. 2018. Silencing ARHGAP9 correlates with the risk of breast cancer and inhibits the proliferation, migration, and invasion of breast cancer. In Journal of cellular biochemistry, 119, 7747-7756. doi:10.1002/jcb.27127. https://pubmed.ncbi.nlm.nih.gov/29905031/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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