C57BL/6NCya-Nlrp3em1flox/Cya
Common Name:
Nlrp3-flox
Product ID:
S-CKO-06043
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Nlrp3-flox
Strain ID
CKOCMP-216799-Nlrp3-B6N-VA
Gene Name
Product ID
S-CKO-06043
Gene Alias
AGTAVPRL; AII/AVP; Cias1; FCAS; FCU; MWS; Mmig1; NALP3; Pypaf1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Nlrp3em1flox/Cya mice (Catalog S-CKO-06043) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000101148
NCBI RefSeq
NM_145827
Target Region
Exon 4
Size of Effective Region
~2.8 kb
Detailed Document
Overview of Gene Research
Nlrp3, also known as NACHT, leucine-rich repeat (LRR), and pyrin domain (PYD)-containing protein 3, is a key component of the NLRP3 inflammasome. The NLRP3 inflammasome is a multi-protein signaling complex that triggers the activation of inflammatory caspases and the maturation of interleukin-1β, playing a crucial role in the innate immune response. It is involved in various biological processes and is associated with numerous human autoinflammatory, autoimmune diseases, as well as other conditions such as cancer, acute liver injury, and cardiovascular diseases [2,5,6,7].
Mitophagy/autophagy blockade leads to the accumulation of damaged, ROS-generating mitochondria, which in turn activates the NLRP3 inflammasome. Inhibition of the voltage-dependent anion channel, which dysregulates mitochondrial activity, suppresses both ROS generation and inflammasome activation, indicating that NLRP3 inflammasome senses mitochondrial dysfunction [1]. Different NLRP3 stimuli lead to the disassembly of the trans-Golgi network (TGN), and NLRP3 is recruited to the dispersed TGN through an ionic bond with phosphatidylinositol-4-phosphate (PtdIns4P), leading to its aggregation and downstream signaling activation [3]. Palmitoylation of NLRP3 Cys126 by ZDHHC7 promotes NLRP3-mediated inflammasome activation, and perturbation of this palmitoylation diminishes NLRP3 activation in macrophages and in vivo [4]. In the context of acute liver injury, aberrant activation of the NLRP3 inflammasome is involved in various types of injury, and its activation is linked to programmed cell death [6]. In cardiovascular diseases, NLRP3 is sensitive to danger signals like ischemia and alarmins, and its activation regulates cell survival through caspase-1 and gasdermin-D [7].
In conclusion, Nlrp3, as a key part of the NLRP3 inflammasome, is essential in the innate immune response and inflammation-related processes. Model-based research, especially studies using gene knockout or conditional knockout mouse models (although not specifically detailed in these abstracts but generally important in such research), has helped to reveal its role in multiple disease areas such as inflammation-related diseases, cancer, acute liver injury, and cardiovascular diseases. Understanding Nlrp3 is crucial for developing therapeutic strategies against these diseases.
References:
1. Zhou, Rongbin, Yazdi, Amir S, Menu, Philippe, Tschopp, Jürg. 2010. A role for mitochondria in NLRP3 inflammasome activation. In Nature, 469, 221-5. doi:10.1038/nature09663. https://pubmed.ncbi.nlm.nih.gov/21124315/
2. Jo, Eun-Kyeong, Kim, Jin Kyung, Shin, Dong-Min, Sasakawa, Chihiro. 2015. Molecular mechanisms regulating NLRP3 inflammasome activation. In Cellular & molecular immunology, 13, 148-59. doi:10.1038/cmi.2015.95. https://pubmed.ncbi.nlm.nih.gov/26549800/
3. Chen, Jueqi, Chen, Zhijian J. 2018. PtdIns4P on dispersed trans-Golgi network mediates NLRP3 inflammasome activation. In Nature, 564, 71-76. doi:10.1038/s41586-018-0761-3. https://pubmed.ncbi.nlm.nih.gov/30487600/
4. Yu, Tao, Hou, Dan, Zhao, Jiaqi, Linder, Maurine E, Lin, Hening. 2024. NLRP3 Cys126 palmitoylation by ZDHHC7 promotes inflammasome activation. In Cell reports, 43, 114070. doi:10.1016/j.celrep.2024.114070. https://pubmed.ncbi.nlm.nih.gov/38583156/
5. Tengesdal, Isak W, Dinarello, Charles A, Marchetti, Carlo. 2023. NLRP3 and cancer: Pathogenesis and therapeutic opportunities. In Pharmacology & therapeutics, 251, 108545. doi:10.1016/j.pharmthera.2023.108545. https://pubmed.ncbi.nlm.nih.gov/37866732/
6. Yu, Chaoqun, Chen, Peng, Miao, Longyu, Di, Guohu. 2023. The Role of the NLRP3 Inflammasome and Programmed Cell Death in Acute Liver Injury. In International journal of molecular sciences, 24, . doi:10.3390/ijms24043067. https://pubmed.ncbi.nlm.nih.gov/36834481/
7. Mezzaroma, Eleonora, Abbate, Antonio, Toldo, Stefano. 2021. NLRP3 Inflammasome Inhibitors in Cardiovascular Diseases. In Molecules (Basel, Switzerland), 26, . doi:10.3390/molecules26040976. https://pubmed.ncbi.nlm.nih.gov/33673188/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen