C57BL/6JCya-Trim47em1flox/Cya
Common Name:
Trim47-flox
Product ID:
S-CKO-06128
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Trim47-flox
Strain ID
CKOCMP-217333-Trim47-B6J-VA
Gene Name
Product ID
S-CKO-06128
Gene Alias
2210023F24Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trim47em1flox/Cya mice (Catalog S-CKO-06128) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021120
NCBI RefSeq
NM_001205081
Target Region
Exon 2~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
TRIM47, a member of the tripartite motif-containing protein family, functions as an E3 ubiquitin ligase. It is involved in multiple biological processes and signaling pathways, such as the NF-κB, MAPK, and STAT3 signaling pathways, and plays a significant role in various diseases [1,3,4,5,6,7,8]. Genetic models, like knockout (KO) or conditional knockout (CKO) mouse models, are valuable for studying its functions.
In LPS-induced acute lung injury in mice, TRIM47-deficient mice were resistant to the injury and death, with attenuated pulmonary inflammation. TRIM47 promoted the K63-linked ubiquitination of TRAF2, activating NF-κB and MAPK signaling pathways in endothelial cells [1]. In hepatocellular carcinoma, TRIM47 knockdown led to HCC ferroptosis induction, mainly through CDO1-mediated regulation of GSH synthesis [2]. In gastric cancer, knockdown of TRIM47 inhibited cell proliferation, migration, and invasion, while overexpression promoted these behaviors, and TRIM47 was found to interact with CYLD protein, promoting its K48-linked ubiquitination and degradation [4]. In prostate cancer, TRIM47 knockdown inhibited cell proliferation, cell cycle, migration, and invasion, and promoted apoptosis, and TRIM47 was shown to bind to MDM2 and regulate MDM2/p53 expression [7]. In ovarian cancer, TRIM47 knockdown suppressed cell proliferation, invasion, migration, and epithelial-mesenchymal transition, and inhibited tumor growth in xenograft assays, by suppressing STAT3 phosphorylation [8].
In conclusion, TRIM47 is an E3 ubiquitin ligase that significantly impacts disease-related biological processes. Model-based research, especially using KO/CKO mouse models, has revealed its role in diseases like acute lung injury, hepatocellular carcinoma, gastric cancer, prostate cancer, and ovarian cancer. These findings enhance our understanding of disease mechanisms and suggest TRIM47 as a potential therapeutic target.
References:
1. Qian, Yisong, Wang, Ziwei, Lin, Hongru, Fu, Mingui, Xin, Hong-Bo. 2022. TRIM47 is a novel endothelial activation factor that aggravates lipopolysaccharide-induced acute lung injury in mice via K63-linked ubiquitination of TRAF2. In Signal transduction and targeted therapy, 7, 148. doi:10.1038/s41392-022-00953-9. https://pubmed.ncbi.nlm.nih.gov/35513381/
2. Zhang, Jie, Yimamu, Malire, Cheng, Ziqi, Wu, Jianye, Guo, Chuanyong. 2024. TRIM47-CDO1 axis dictates hepatocellular carcinoma progression by modulating ferroptotic cell death through the ubiquitin‒proteasome system. In Free radical biology & medicine, 219, 31-48. doi:10.1016/j.freeradbiomed.2024.04.222. https://pubmed.ncbi.nlm.nih.gov/38614226/
3. Azuma, Kotaro, Inoue, Satoshi. 2022. Efp/TRIM25 and Its Related Protein, TRIM47, in Hormone-Dependent Cancers. In Cells, 11, . doi:10.3390/cells11152464. https://pubmed.ncbi.nlm.nih.gov/35954308/
4. Wang, Jianguo, Ye, Jing, Liu, Rongqiang, Chen, Chen, Wang, Weixing. 2024. TRIM47 drives gastric cancer cell proliferation and invasion by regulating CYLD protein stability. In Biology direct, 19, 106. doi:10.1186/s13062-024-00555-1. https://pubmed.ncbi.nlm.nih.gov/39516831/
5. Zhan, Wenjuan, Zhang, Huifang, Su, Yufei, Yin, Li. 2024. TRIM47 promotes HDM-induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF-κB/NLRP3 signaling. In Cell biology international, 48, 1138-1147. doi:10.1002/cbin.12186. https://pubmed.ncbi.nlm.nih.gov/38769645/
6. Xia, YuJian, Wei, ZheWei, Huang, WeiBin, Wei, XiaoJing, He, YuLong. 2020. Trim47 overexpression correlates with poor prognosis in gastric cancer. In Neoplasma, 68, 307-316. doi:10.4149/neo_2020_200708N706. https://pubmed.ncbi.nlm.nih.gov/33350849/
7. Wang, Chengyong, Chang, Rui, Li, Jian, Li, Liqiang. 2024. TRIM47 silencing inhibits the malignant biological behaviors of prostate cancer cells by regulating MDM2/p53 signaling. In Cell biochemistry and biophysics, 82, 1567-1578. doi:10.1007/s12013-024-01308-7. https://pubmed.ncbi.nlm.nih.gov/38802602/
8. Wang, Xi, Fu, Yu, Xing, Yanyan. 2022. TRIM47 promotes ovarian cancer cell proliferation, migration, and invasion by activating STAT3 signaling. In Clinics (Sao Paulo, Brazil), 77, 100122. doi:10.1016/j.clinsp.2022.100122. https://pubmed.ncbi.nlm.nih.gov/36288633/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen