C57BL/6JCya-Otop1em1flox/Cya
Common Name:
Otop1-flox
Product ID:
S-CKO-06348
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Otop1-flox
Strain ID
CKOCMP-21906-Otop1-B6J-VA
Gene Name
Product ID
S-CKO-06348
Gene Alias
A530025J20Rik; Otp1; tlt
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Otop1em1flox/Cya mice (Catalog S-CKO-06348) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000114099
NCBI RefSeq
NM_172709
Target Region
Exon 2
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Otop1, or Otopetrin 1, is a proton-selective ion channel with multiple essential functions. It is involved in sour taste sensing in taste receptor cells, the formation of otoconia in the inner ear, and potentially in alkaline sensation in vertebrates [1,3,5]. It participates in the transduction of taste signals, a crucial pathway for organisms to sense and respond to various taste stimuli [4].
In gene knockout studies, Otop1-/-mouse strains have provided significant insights. In taste-related research, taste responses to NH4Cl measured from isolated Type III taste receptor cells (TRCs) or gustatory nerves were strongly attenuated or eliminated in Otop1-/-mice, revealing its role in mediating a major component of the taste of NH4Cl [2]. Also, knockout of Otop1 eliminated acid responses from sour-sensing TRCs, indicating its essentiality for sour sensing in the taste system [3].
In conclusion, Otop1 is a key ion channel involved in taste-related sensing processes such as sour and ammonium taste detection. The use of Otop1 knockout mouse models has been instrumental in uncovering its role in taste transduction, which may have implications for understanding taste-related disorders and potentially developing novel taste-modifying therapies.
References：
1. Tian, Lifeng, Zhang, Hao, Yang, Shilong, Luo, Lei, Lai, Ren. 2023. Vertebrate OTOP1 is also an alkali-activated channel. In Nature communications, 14, 26. doi:10.1038/s41467-022-35754-9. https://pubmed.ncbi.nlm.nih.gov/36596786/
2. Liang, Ziyu, Wilson, Courtney E, Teng, Bochuan, Kinnamon, Sue C, Liman, Emily R. 2023. The proton channel OTOP1 is a sensor for the taste of ammonium chloride. In Nature communications, 14, 6194. doi:10.1038/s41467-023-41637-4. https://pubmed.ncbi.nlm.nih.gov/37798269/
3. Zhang, Jin, Jin, Hao, Zhang, Wenyi, Ye, Mingyu, Zuker, Charles S. 2019. Sour Sensing from the Tongue to the Brain. In Cell, 179, 392-402.e15. doi:10.1016/j.cell.2019.08.031. https://pubmed.ncbi.nlm.nih.gov/31543264/
4. Taruno, Akiyuki, Nomura, Kengo, Kusakizako, Tsukasa, Nureki, Osamu, Foskett, J Kevin. 2020. Taste transduction and channel synapses in taste buds. In Pflugers Archiv : European journal of physiology, 473, 3-13. doi:10.1007/s00424-020-02464-4. https://pubmed.ncbi.nlm.nih.gov/32936320/
5. Islam, Md Mominul, Sasaki, Omi, Yano-Nashimoto, Saori, Okamatsu-Ogura, Yuko, Yamaguchi, Soichiro. 2023. Cibacron blue 3G-A is a novel inhibitor of Otopetrin 1 (OTOP1), a proton channel. In Biochemical and biophysical research communications, 665, 64-70. doi:10.1016/j.bbrc.2023.04.112. https://pubmed.ncbi.nlm.nih.gov/37149984/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen