C57BL/6JCya-Trp53em1flox/Cya
Common Name:
Trp53-flox
Product ID:
S-CKO-06450
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trp53-flox
Strain ID
CKOCMP-22059-Trp53-B6J-VB
Gene Name
Product ID
S-CKO-06450
Gene Alias
Tp53; bbl; bfy; bhy; p44; p53
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trp53em1flox/Cya mice (Catalog S-CKO-06450) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108658
NCBI RefSeq
NM_011640
Target Region
Exon 2~10
Size of Effective Region
~4.5 kb
Detailed Document
Overview of Gene Research
Trp53, the mouse ortholog of the human TP53 gene, is a crucial tumor suppressor gene. It plays extensive roles in regulating the cell cycle, DNA repair, apoptosis, cellular metabolism, and senescence, acting as the "guardian of the genome" [2]. Mutations or deletions of Trp53 lead to loss of its normal function, which is associated with the development of many cancers. Mouse models are valuable for studying Trp53's function in vivo.
Trp53 -deficient mice have provided insights into its role in breast cancer. The original Trp53 -knockout mice showed no mammary gland phenotype, but BALB/c-Trp53 -deficient mice demonstrated a delayed involution phenotype and a mammary tumor phenotype. This indicates the importance of Trp53 in mammary gland involution and the development of breast cancer [1]. Additionally, in the adrenal medulla, dual loss of Rb1 and Trp53 led to the development of spontaneous pheochromocytomas, suggesting that Trp53 is an efficient genetic modifier of Rb1 loss in the development of this tumor type [3].
In conclusion, Trp53 is essential for maintaining normal cellular homeostasis and preventing tumorigenesis. Mouse models, especially those with Trp53 knockout or conditional knockout, have significantly contributed to our understanding of its role in diseases such as breast cancer and pheochromocytoma, providing valuable insights into the mechanisms of tumor development and potential therapeutic targets.
References:
1. Blackburn, Anneke C, Jerry, D Joseph. 2002. Knockout and transgenic mice of Trp53: what have we learned about p53 in breast cancer? In Breast cancer research : BCR, 4, 101-11. doi:. https://pubmed.ncbi.nlm.nih.gov/12052252/
2. Merino, Diana, Malkin, David. . p53 and hereditary cancer. In Sub-cellular biochemistry, 85, 1-16. doi:10.1007/978-94-017-9211-0_1. https://pubmed.ncbi.nlm.nih.gov/25201186/
3. Tonks, Ian D, Mould, Arne W, Schroder, Wayne A, Walker, Graeme J, Kay, Graham F. . Dual loss of rb1 and Trp53 in the adrenal medulla leads to spontaneous pheochromocytoma. In Neoplasia (New York, N.Y.), 12, 235-43. doi:. https://pubmed.ncbi.nlm.nih.gov/20234817/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen