C57BL/6JCya-Trpc6em1flox/Cya
Common Name:
Trpc6-flox
Product ID:
S-CKO-06458
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Trpc6-flox
Strain ID
CKOCMP-22068-Trpc6-B6J-VA
Gene Name
Product ID
S-CKO-06458
Gene Alias
LLHWJM002; LLHWJM003; LLHWJM004; TRP-6; Trrp6; mtrp6
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trpc6em1flox/Cya mice (Catalog S-CKO-06458) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000050433
NCBI RefSeq
NM_013838
Target Region
Exon 2
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Trpc6 is a Ca(2+)-permeable non-selective cation channel. It belongs to the DAG-activated subfamily of channels and may also be involved in the store-operated calcium entry pathway [1,2]. It is expressed in various tissues such as the brain, smooth-muscle-containing tissues, kidney, immune and blood cells, and is crucial for many physiological processes [1].
Studies with Trpc6(-/-) mice suggest its role in regulating vascular and pulmonary smooth muscle contraction [1]. In the kidney, Trpc6 is an essential component of the slit diaphragm architecture of podocytes. Mutations and over-activation of Trpc6 lead to glomeruli injury. In diabetic kidney disease (DKD) models, factors like angiotensin II stimulate calcium influx through Trpc6, causing podocyte hypertrophy and foot process effacement, and ultimately albuminuria and renal injury [3]. Trpc6 knockout reduces glomerular manifestations of disease in some models and renal fibrosis caused by urinary tract obstruction, but is less effective at reducing DKD in mouse and rat models [4].
In conclusion, Trpc6 plays important physiological roles in smooth muscle contraction and kidney function, especially in maintaining the structure and function of podocytes. Gene knockout mouse models have revealed its significance in diseases like DKD and other renal disorders, providing insights into potential therapeutic targets for these diseases.
References:
1. Dietrich, A, Gudermann, T. . TRPC6. In Handbook of experimental pharmacology, , 125-41. doi:. https://pubmed.ncbi.nlm.nih.gov/17217054/
2. Jardin, Isaac, Nieto, Joel, Salido, Ginés M, Rosado, Juan A. 2020. TRPC6 channel and its implications in breast cancer: an overview. In Biochimica et biophysica acta. Molecular cell research, 1867, 118828. doi:10.1016/j.bbamcr.2020.118828. https://pubmed.ncbi.nlm.nih.gov/32822726/
3. Staruschenko, Alexander, Spires, Denisha, Palygin, Oleg. 2019. Role of TRPC6 in Progression of Diabetic Kidney Disease. In Current hypertension reports, 21, 48. doi:10.1007/s11906-019-0960-9. https://pubmed.ncbi.nlm.nih.gov/31115705/
4. Dryer, Stuart E, Kim, Eun Young. 2022. The Effects of TRPC6 Knockout in Animal Models of Kidney Disease. In Biomolecules, 12, . doi:10.3390/biom12111710. https://pubmed.ncbi.nlm.nih.gov/36421724/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen