C57BL/6JCya-Cerkem1flox/Cya
Common Name:
Cerk-flox
Product ID:
S-CKO-06654
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cerk-flox
Strain ID
CKOCMP-223753-Cerk-B6J-VA
Gene Name
Product ID
S-CKO-06654
Gene Alias
D330016D08Rik; mKIAA1646
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cerkem1flox/Cya mice (Catalog S-CKO-06654) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044332
NCBI RefSeq
NM_145475
Target Region
Exon 6
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Ceramide kinase (CERK) is a mammalian lipid kinase that phosphorylates ceramide to produce ceramide-1-phosphate (C1P) [4,5]. C1P is involved in various biological processes such as membrane biology, regulation of membrane-bound proteins, and acts as a signaling entity [5]. It has been associated with multiple pathways including those related to cell survival, lipid metabolism, and redox homeostasis [4]. Genetic models like CERK-knockout mice are valuable for studying its functions [1].
In a study using CERK-knockout mice, it was found that CERK-derived C1P plays a protective role against high-altitude pulmonary edema (HAPE). Inhibition of CERK exacerbated HAPE induced by a hypobaric hypoxic environment. CERK inhibition led to aryl hydrocarbon receptor nuclear translocator-like (ARNTL) autophagic degradation, disrupting the circadian rhythm and triggering mitochondrial damage. Exogenous C1P prevented ARNTL degradation, alleviated mitochondrial damage, and relieved HAPE [1]. In breast cancer, overexpression of CERK was associated with nodal metastasis, late tumor stage, high proliferation potency, and poor patient survival. Computational network analysis showed its association with metastasis and drug-resistance markers [2]. In mutant KRAS non-small cell lung cancer (NSCLC), inhibition of CERK induced ferroptosis, and it synergized with cisplatin in reducing cell survival and in vivo tumor growth [3].
In conclusion, CERK is crucial in maintaining physiological functions through its role in producing C1P. Model-based research, especially using CERK-knockout mouse models, has revealed its significance in diseases such as HAPE, breast cancer, and NSCLC. Understanding CERK functions provides potential therapeutic targets for these diseases.
References:
1. Tian, Liuyang, Zhao, Chenghui, Yan, Yan, He, Kunlun, Zhao, Xiaojing. 2023. Ceramide-1-phosphate alleviates high-altitude pulmonary edema by stabilizing circadian ARNTL-mediated mitochondrial dynamics. In Journal of advanced research, 60, 75-92. doi:10.1016/j.jare.2023.07.008. https://pubmed.ncbi.nlm.nih.gov/37479181/
2. Bhadwal, Priyanka, Randhawa, Vinay, Vaiphei, Kim, Dahiya, Divya, Agnihotri, Navneet. 2022. Clinical relevance of CERK and SPHK1 in breast cancer and their association with metastasis and drug resistance. In Scientific reports, 12, 18239. doi:10.1038/s41598-022-20976-0. https://pubmed.ncbi.nlm.nih.gov/36309544/
3. Vu, Ngoc T, Kim, Minjung, Stephenson, Daniel J, MacKnight, H Patrick, Chalfant, Charles E. . Ceramide Kinase Inhibition Drives Ferroptosis and Sensitivity to Cisplatin in Mutant KRAS Lung Cancer by Dysregulating VDAC-Mediated Mitochondria Function. In Molecular cancer research : MCR, 20, 1429-1442. doi:10.1158/1541-7786.MCR-22-0085. https://pubmed.ncbi.nlm.nih.gov/35560154/
4. Dong, Wei, Li, Qing, Lu, Xing, Zhang, Chong, Jin, Junfei. 2024. Ceramide kinase-mediated C1P metabolism attenuates acute liver injury by inhibiting the interaction between KEAP1 and NRF2. In Experimental & molecular medicine, 56, 946-958. doi:10.1038/s12276-024-01203-4. https://pubmed.ncbi.nlm.nih.gov/38556546/
5. Bornancin, Frédéric. 2010. Ceramide kinase: the first decade. In Cellular signalling, 23, 999-1008. doi:10.1016/j.cellsig.2010.11.012. https://pubmed.ncbi.nlm.nih.gov/21111813/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen