C57BL/6JCya-Wbp2em1flox/Cya
Common Name:
Wbp2-flox
Product ID:
S-CKO-06662
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Wbp2-flox
Strain ID
CKOCMP-22378-Wbp2-B6J-VA
Gene Name
Product ID
S-CKO-06662
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Wbp2em1flox/Cya mice (Catalog S-CKO-06662) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000074628
NCBI RefSeq
NM_016852
Target Region
Exon 2
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
WBP2, also known as WW domain-binding protein 2, is an oncogenic transcriptional co-factor. It interacts with multiple key signaling pathways such as ER/PR, EGFR, PI3K, Hippo, and Wnt in cancer [3]. In the Hippo pathway, it associates with transcriptional co-activators YAP and TAZ, and also modulates upstream Hippo components like LATS2 and WWC3 [2]. Additionally, it has a role in miRNA biogenesis by interacting with the microprocessor complex [4].
In cisplatin-induced acute kidney injury (CP-AKI), WBP2, highly expressed in renal proximal tubular cells, was found to be downregulated. Mechanistically, WBP2 interacted with GPX4 via its PPXY1 motif, competing with HSC70 for binding to GPX4's KEFRQ-like motifs, thus inhibiting chaperon-mediated autophagy of GPX4 and decelerating ferroptosis to alleviate CP-AKI [1]. In triple-negative breast cancer (TNBC), WBP2 enhanced cell migration and invasion under TNF-α stimulation. It potentiated TNF-α-induced NF-κB transcriptional activity and nuclear localization by promoting ubiquitin-mediated proteasomal degradation of NFKBIA. WBP2 also induced mRNA stability of BTRC, which targets NFKBIA for ubiquitination and degradation [5].
In conclusion, WBP2 is involved in multiple crucial biological processes and disease conditions. In CP-AKI, it plays a protective role by inhibiting ferroptosis. In breast cancer, especially TNBC, it promotes cancer cell aggressiveness through modulating specific signaling pathways. These findings from functional studies, such as those in relevant disease models, enhance our understanding of WBP2's functions and provide potential therapeutic targets for related diseases.
References:
1. Deng, Zebin, Wang, Yilong, Liu, Jiachen, Dai, Yingbo, Deng, Fei. 2023. WBP2 restrains the lysosomal degradation of GPX4 to inhibit ferroptosis in cisplatin-induced acute kidney injury. In Redox biology, 65, 102826. doi:10.1016/j.redox.2023.102826. https://pubmed.ncbi.nlm.nih.gov/37516014/
2. Lim, Yvonne Xinyi, Lin, Hexian, Seah, Sock Hong, Lim, Yoon Pin. 2021. Reciprocal Regulation of Hippo and WBP2 Signalling-Implications in Cancer Therapy. In Cells, 10, . doi:10.3390/cells10113130. https://pubmed.ncbi.nlm.nih.gov/34831354/
3. Tabatabaeian, Hossein, Rao, Angad, Ramos, Alisha, Sudol, Marius, Lim, Yoon Pin. 2020. The emerging roles of WBP2 oncogene in human cancers. In Oncogene, 39, 4621-4635. doi:10.1038/s41388-020-1318-0. https://pubmed.ncbi.nlm.nih.gov/32393834/
4. Tabatabaeian, Hossein, Lim, Shen Kiat, Chu, Tinghine, Seah, Sock Hong, Lim, Yoon Pin. 2021. WBP2 inhibits microRNA biogenesis via interaction with the microprocessor complex. In Life science alliance, 4, . doi:10.26508/lsa.202101038. https://pubmed.ncbi.nlm.nih.gov/34117091/
5. Lim, Yvonne Xinyi, Lin, Hexian, Chu, Tinghine, Lim, Yoon Pin. 2021. WBP2 promotes BTRC mRNA stability to drive migration and invasion in triple-negative breast cancer via NF-κB activation. In Molecular oncology, 16, 422-446. doi:10.1002/1878-0261.13048. https://pubmed.ncbi.nlm.nih.gov/34197030/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen