C57BL/6JCya-Dus3lem1flox/Cya
Common Name:
Dus3l-flox
Product ID:
S-CKO-06797
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dus3l-flox
Strain ID
CKOCMP-224907-Dus3l-B6J-VA
Gene Name
Product ID
S-CKO-06797
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dus3lem1flox/Cya mice (Catalog S-CKO-06797) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007747
NCBI RefSeq
NM_144858.2
Target Region
Exon 2
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Dus3l, short for Dihydrouridine Synthase 3-Like, is a dihydrouridine synthase homolog that mediates dihydrouridylation, an epitranscriptomic RNA modification which can regulate RNA activity [1]. RNA modifications play crucial roles in regulating RNA structure, folding, and protein-RNA interactions, and are involved in fundamental gene expression processes [2].
In one study, using a chemoproteomic strategy RNABPP, researchers found that DUS3L-knockout (KO) cells have compromised protein translation rates and impaired cellular proliferation, suggesting its importance in these cellular processes [1]. DUS3L was also identified among genes containing rare, potentially deleterious variants transmitted to affected first-degree relatives in multiple families with inflammatory bowel disease, indicating a possible genetic link to this disease [3]. In an integrative analysis of transcriptome-wide association study and mRNA expression profile, DUS3L was identified as an overlapping gene associated with aortic aneurysm and dissection [4]. In acute myocardial infarction patients, DUS3L was among the hub genes in a specific co-expression module [5].
In conclusion, DUS3L has significant functions in processes like protein translation and cellular proliferation. Its potential involvement in diseases such as inflammatory bowel disease, aortic aneurysm and dissection, and acute myocardial infarction, as revealed through gene knockout and genetic analysis in relevant models, highlights its importance in understanding the molecular mechanisms underlying these diseases.
References:
1. Dai, Wei, Li, Ang, Yu, Nathan J, Wühr, Martin, Kleiner, Ralph E. 2021. Activity-based RNA-modifying enzyme probing reveals DUS3L-mediated dihydrouridylation. In Nature chemical biology, 17, 1178-1187. doi:10.1038/s41589-021-00874-8. https://pubmed.ncbi.nlm.nih.gov/34556860/
2. Dai, Wei, Yu, Nathan J, Kleiner, Ralph E. 2023. Chemoproteomic Approaches to Studying RNA Modification-Associated Proteins. In Accounts of chemical research, 56, 2726-2739. doi:10.1021/acs.accounts.3c00450. https://pubmed.ncbi.nlm.nih.gov/37733063/
3. Park, Yoo Min, Ha, Eunji, Gu, Ki-Nam, Kim, Kwangwoo, Kim, Hyo Jong. . Host Genetic and Gut Microbial Signatures in Familial Inflammatory Bowel Disease. In Clinical and translational gastroenterology, 11, e00213. doi:10.14309/ctg.0000000000000213. https://pubmed.ncbi.nlm.nih.gov/32764209/
4. Zhang, Yiran, Li, Lin, Ma, Liang. 2021. Integrative analysis of transcriptome-wide association study and mRNA expression profile identified candidate genes and pathways associated with aortic aneurysm and dissection. In Gene, 808, 145993. doi:10.1016/j.gene.2021.145993. https://pubmed.ncbi.nlm.nih.gov/34626721/
5. Zhang, Baojian, Li, Biao, Sun, Chao, Xiao, Yichao, Liu, Qiming. 2021. Identification of key gene modules and pathways of human platelet transcriptome in acute myocardial infarction patients through co-expression network. In American journal of translational research, 13, 3890-3905. doi:. https://pubmed.ncbi.nlm.nih.gov/34017580/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen