C57BL/6JCya-Slc30a10em1flox/Cya
Common Name:
Slc30a10-flox
Product ID:
S-CKO-06987
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Slc30a10-flox
Strain ID
CKOCMP-226781-Slc30a10-B6J-VA
Gene Name
Product ID
S-CKO-06987
Gene Alias
E130106K10Rik; Gm212
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Slc30a10em1flox/Cya mice (Catalog S-CKO-06987) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000061093
NCBI RefSeq
NM_001033286
Target Region
Exon 3~4
Size of Effective Region
~6.3 kb
Detailed Document
Overview of Gene Research
Slc30a10, a manganese (Mn) efflux transporter, is crucial for maintaining Mn homeostasis. It exports Mn from hepatocytes into bile and from enterocytes into the lumen of the gastrointestinal tract, thus playing a key role in physiological Mn excretion [1,3,4]. Mn is an essential metal, but excess can lead to toxicity, and Slc30a10 is vital in preventing such toxicity. Genetic models, like knockout (KO) mice, have been instrumental in studying its function.
Whole-body Slc30a10-deficient mice develop severe Mn excess and impaired systemic and biliary Mn excretion, highlighting the gene's essential role in Mn excretion by hepatocytes and enterocytes [1]. Mice with liver-specific or small-intestine-specific Slc30a10 deficiency develop less severe Mn excess, suggesting additional sites of Slc30a10 expression contribute to Mn homeostasis [1]. Pan-neuronal/glial Slc30a10 knockout mice show elevated Mn levels in specific brain regions during early postnatal development and neuromotor deficits in adolescence or adulthood, indicating its role in regulating brain Mn levels and protecting against neuromotor and dopaminergic neurotransmission deficits [2].
In conclusion, Slc30a10 is essential for Mn excretion in hepatocytes and enterocytes and for regulating Mn levels in the brain, especially during early postnatal development. The study of Slc30a10 using KO mouse models has enhanced our understanding of Mn-related diseases such as hereditary Mn-induced neuromotor disease, highlighting its potential as a target for treating Mn toxicity [1,2].
References:
1. Mercadante, Courtney J, Prajapati, Milankumar, Conboy, Heather L, Rao, Deepa B, Bartnikas, Thomas B. . Manganese transporter Slc30a10 controls physiological manganese excretion and toxicity. In The Journal of clinical investigation, 129, 5442-5461. doi:10.1172/JCI129710. https://pubmed.ncbi.nlm.nih.gov/31527311/
2. Taylor, Cherish A, Grant, Stephanie M, Jursa, Thomas, Gonzales, Rueben A, Mukhopadhyay, Somshuvra. . SLC30A10 manganese transporter in the brain protects against deficits in motor function and dopaminergic neurotransmission under physiological conditions. In Metallomics : integrated biometal science, 15, . doi:10.1093/mtomcs/mfad021. https://pubmed.ncbi.nlm.nih.gov/36990693/
3. Mukhopadhyay, Somshuvra. 2017. Familial manganese-induced neurotoxicity due to mutations in SLC30A10 or SLC39A14. In Neurotoxicology, 64, 278-283. doi:10.1016/j.neuro.2017.07.030. https://pubmed.ncbi.nlm.nih.gov/28789954/
4. Prajapati, Milankumar, Zhang, Jared Z, Chiu, Lauren, Aghajan, Mariam, Bartnikas, Thomas B. 2024. Hepatic HIF2 is a key determinant of manganese excess and polycythemia in SLC30A10 deficiency. In JCI insight, 9, . doi:10.1172/jci.insight.169738. https://pubmed.ncbi.nlm.nih.gov/38652538/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen