C57BL/6JCya-Ampd1em1flox/Cya
Common Name:
Ampd1-flox
Product ID:
S-CKO-07272
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ampd1-flox
Strain ID
CKOCMP-229665-Ampd1-B6J-VA
Gene Name
Product ID
S-CKO-07272
Gene Alias
Ampd-1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ampd1em1flox/Cya mice (Catalog S-CKO-07272) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000090715
NCBI RefSeq
NM_001033303
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Ampd1, encoding the skeletal muscle-specific isoform of adenosine monophosphate deaminase, plays a role in skeletal muscle metabolism. It may be involved in pathways related to energy regulation, as it catalyzes the conversion of AMP to IMP and NH3, potentially influencing cellular energy states. Understanding its function is important for fields such as sports genomics and disease research related to muscle function and metabolism [3,4].
In gene-related studies, Ampd1-deficient homozygous mice showed a less severe state of insulin resistance, improved glucose tolerance, and enhanced insulin clearance when fed a high-fat diet. This indicates that Ampd1 may be a key regulator in insulin-related metabolic pathways [1]. In addition, in Ampd1-deficient mice challenged with a high-fat diet, phosphorylation levels of AMPK, Akt, and p70 S6 kinase in skeletal muscle were higher compared to wild-type mice, suggesting that Ampd1 deficiency activates the AMPK/Akt/mTORC1/p70 S6 kinase axis in skeletal muscle, which may contribute to improving insulin resistance [2]. In another study, knockdown of Ampd1 in mouse skeletal muscle affected muscle function, slowing contraction and relaxation kinetics in EDL muscle but not SOL muscle, and also modulated the [AMP]/AMPK responses to fatiguing contractions in an intensity-dependent manner in EDL muscle [3].
In conclusion, Ampd1 is crucial for skeletal muscle metabolism and energy-related processes. Studies using Ampd1-deficient mouse models have revealed its significance in insulin resistance-related metabolic diseases. These findings provide insights into potential therapeutic targets for diseases such as type 2 diabetes and also have implications for understanding muscle function in the context of exercise and athletic performance [1,2,3].
References:
1. Cheng, Jidong, Morisaki, Hiroko, Toyama, Keiko, Holmes, Edward W, Morisaki, Takayuki. 2014. AMPD1: a novel therapeutic target for reversing insulin resistance. In BMC endocrine disorders, 14, 96. doi:10.1186/1472-6823-14-96. https://pubmed.ncbi.nlm.nih.gov/25511531/
2. Tandelilin, Andreas A K, Hirase, Tetsuaki, Hudoyo, Athanasius W, Morisaki, Hiroko, Morisaki, Takayuki. 2015. AMPD1 regulates mTORC1-p70 S6 kinase axis in the control of insulin sensitivity in skeletal muscle. In BMC endocrine disorders, 15, 11. doi:10.1186/s12902-015-0010-9. https://pubmed.ncbi.nlm.nih.gov/25887856/
3. Hafen, Paul S, Law, Andrew S, Matias, Catalina, Miller, Spencer G, Brault, Jeffrey J. 2022. Skeletal muscle contraction kinetics and AMPK responses are modulated by the adenine nucleotide degrading enzyme AMPD1. In Journal of applied physiology (Bethesda, Md. : 1985), 133, 1055-1066. doi:10.1152/japplphysiol.00035.2022. https://pubmed.ncbi.nlm.nih.gov/36107988/
4. Ginevičienė, Valentina, Jakaitienė, Audronė, Pranculis, Aidas, Tubelis, Linas, Utkus, Algirdas. 2014. AMPD1 rs17602729 is associated with physical performance of sprint and power in elite Lithuanian athletes. In BMC genetics, 15, 58. doi:10.1186/1471-2156-15-58. https://pubmed.ncbi.nlm.nih.gov/24885427/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen