C57BL/6JCya-Gpr75em1flox/Cya
Common Name:
Gpr75-flox
Product ID:
S-CKO-07944
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gpr75-flox
Strain ID
CKOCMP-237716-Gpr75-B6J-VA
Gene Name
Product ID
S-CKO-07944
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpr75em1flox/Cya mice (Catalog S-CKO-07944) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000109430
NCBI RefSeq
NM_175490
Target Region
Exon 2
Size of Effective Region
~2.0 kb
Detailed Document
Overview of Gene Research
Gpr75, a G protein-coupled receptor, has been identified as the high-affinity receptor of 20-hydroxyeicosatetraenoic acid (20-HETE). It is involved in pro-inflammatory and hypertensive signalling cascades, and plays a role in regulating metabolism and glucose homeostasis [2,3,4,5,6].
In Gpr75-deficient mice, several beneficial effects were observed. When fed a high-fat diet (HFD), these mice gained less weight, maintained energy expenditure, and did not develop insulin resistance. Adipose tissue had increased thermogenic gene expression and decreased inflammatory markers. In addition, CRISPR-Cas9-deleted Gpr75 mice fed a palatable western diet adjusted caloric intake to prevent non-alcoholic fatty liver disease (NAFLD) [1,2].
In conclusion, Gpr75 plays a crucial role in metabolism and glucose homeostasis. Gene knockout mouse models have revealed its significance in obesity-driven metabolic disorders and NAFLD, suggesting that inhibiting Gpr75 could potentially be a therapeutic strategy for these diseases.
References：
1. Leeson-Payne, Alasdair, Iyinikkel, Jean, Malcolm, Cameron, Murray, Fiona, Heisler, Lora K. 2024. Loss of GPR75 protects against non-alcoholic fatty liver disease and body fat accumulation. In Cell metabolism, 36, 1076-1087.e4. doi:10.1016/j.cmet.2024.03.016. https://pubmed.ncbi.nlm.nih.gov/38653246/
2. Hossain, Sakib, Gilani, Ankit, Pascale, Jonathan, Garcia, Victor, Schwartzman, Michal Laniado. 2023. Gpr75-deficient mice are protected from high-fat diet-induced obesity. In Obesity (Silver Spring, Md.), 31, 1024-1037. doi:10.1002/oby.23692. https://pubmed.ncbi.nlm.nih.gov/36854900/
3. Dashti, Mohammad Reza, Gorbanzadeh, Fatemeh, Jafari-Gharabaghlou, Davoud, Farhoudi Sefidan Jadid, Mahdi, Zarghami, Nosratollah. 2023. G Protein-Coupled Receptor 75 (GPR75) As a Novel Molecule for Targeted Therapy of Cancer and Metabolic Syndrome. In Asian Pacific journal of cancer prevention : APJCP, 24, 1817-1825. doi:10.31557/APJCP.2023.24.5.1817. https://pubmed.ncbi.nlm.nih.gov/37247305/
4. Fragner, Michael L, Parikh, Manish A, Jackson, Kaedrea A, Frishman, William H, Peterson, Stephen J. 2024. GPR75: A Newly Identified Receptor for Targeted Intervention in the Treatment of Obesity and Metabolic Syndrome. In Cardiology in review, , . doi:10.1097/CRD.0000000000000711. https://pubmed.ncbi.nlm.nih.gov/38695569/
5. Pascale, Jonathan V, Park, Eon Joo, Adebesin, Adeniyi Michael, Schwartzman, Michal Laniado, Garcia, Victor. 2021. Uncovering the signalling, structure and function of the 20-HETE-GPR75 pairing: Identifying the chemokine CCL5 as a negative regulator of GPR75. In British journal of pharmacology, 178, 3813-3828. doi:10.1111/bph.15525. https://pubmed.ncbi.nlm.nih.gov/33974269/
6. Garcia, Victor, Gilani, Ankit, Shkolnik, Brian, Falck, John R, Schwartzman, Michal Laniado. 2017. 20-HETE Signals Through G-Protein-Coupled Receptor GPR75 (Gq) to Affect Vascular Function and Trigger Hypertension. In Circulation research, 120, 1776-1788. doi:10.1161/CIRCRESAHA.116.310525. https://pubmed.ncbi.nlm.nih.gov/28325781/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen