C57BL/6JCya-Abcc4em1flox/Cya
Common Name:
Abcc4-flox
Product ID:
S-CKO-08108
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Abcc4-flox
Strain ID
CKOCMP-239273-Abcc4-B6J-VA
Gene Name
Product ID
S-CKO-08108
Gene Alias
ABCC4-N1; D630049P08Rik; MOATB; MRP4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Abcc4em1flox/Cya mice (Catalog S-CKO-08108) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000036554
NCBI RefSeq
NM_001033336
Target Region
Exon 5~6
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
ABCC4, also known as multi-drug resistance protein-4 (MRP4), is an ATP-binding cassette (ABC) transporter. It plays a crucial role in regulating the transport of endogenous and exogenous substrates, participating in processes like cyclic-adenosine monophosphate (cAMP) homeostasis [1,3,4]. It is involved in multiple biological pathways and is important for the health and function of various tissues [1-9]. Gene knockout (KO) mouse models are valuable for studying its function.
In KO mouse models, Abcc4 deficiency impairs megakaryopoiesis, as fetal liver-derived megakaryocytes from Abcc4-/-mice show impaired proplatelet formation and polyploidization [2]. In the context of cytarabine treatment for acute myeloid leukemia, Abcc4-null mice exhibit enhanced cytarabine-induced hematological toxicity, indicating that ABCC4 protects leukemic and host myeloid cells from cytarabine-mediated insults [5].
In conclusion, ABCC4 is essential for processes such as megakaryopoiesis and protection against certain drug-induced cytotoxicity. The use of Abcc4 KO mouse models has revealed its role in these biological processes and disease-related conditions, providing insights into potential therapeutic strategies for platelet-related disorders and leukemia treatment.
References：
1. Nguyen, Jenny P, Kim, Yechan, Cao, Quynh, Hirota, Jeremy A. 2021. Interactions between ABCC4/MRP4 and ABCC7/CFTR in human airway epithelial cells in lung health and disease. In The international journal of biochemistry & cell biology, 133, 105936. doi:10.1016/j.biocel.2021.105936. https://pubmed.ncbi.nlm.nih.gov/33529712/
2. Ranjit, Sabina, Wang, Yao, Zhu, Jingwen, Naren, Anjaparavanda P, Schuetz, John D. 2023. ABCC4 impacts megakaryopoiesis and protects megakaryocytes against 6-mercaptopurine induced cytotoxicity. In Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy, 72, 101017. doi:10.1016/j.drup.2023.101017. https://pubmed.ncbi.nlm.nih.gov/37988981/
3. Yaneff, Agustín, Sahores, Ana, Gómez, Natalia, Shayo, Carina, Davio, Carlos. . MRP4/ABCC4 As a New Therapeutic Target: Meta-Analysis to Determine cAMP Binding Sites as a Tool for Drug Design. In Current medicinal chemistry, 26, 1270-1307. doi:10.2174/0929867325666171229133259. https://pubmed.ncbi.nlm.nih.gov/29284392/
4. Belleville-Rolland, Tiphaine, Sassi, Yassine, Decouture, Benoit, Gaussem, Pascale, Bachelot-Loza, Christilla. 2016. MRP4 (ABCC4) as a potential pharmacologic target for cardiovascular disease. In Pharmacological research, 107, 381-389. doi:10.1016/j.phrs.2016.04.002. https://pubmed.ncbi.nlm.nih.gov/27063943/
5. Drenberg, C D, Hu, S, Li, L, Sparreboom, A, Baker, S D. 2016. ABCC4 Is a Determinant of Cytarabine-Induced Cytotoxicity and Myelosuppression. In Clinical and translational science, 9, 51-9. doi:10.1111/cts.12366. https://pubmed.ncbi.nlm.nih.gov/26842729/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen