C57BL/6JCya-Sulf1em1flox/Cya
Common Name:
Sulf1-flox
Product ID:
S-CKO-08293
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Sulf1-flox
Strain ID
CKOCMP-240725-Sulf1-B6J-VA
Gene Name
Product ID
S-CKO-08293
Gene Alias
MSulf-1; mKIAA1077
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Sulf1em1flox/Cya mice (Catalog S-CKO-08293) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000177608
NCBI RefSeq
NM_001198565
Target Region
Exon 7
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Sulf1, also known as Sulfatase 1, is an enzyme that modifies heparan sulfate chains of heparan sulfate proteoglycans (HSPGs) [1,2,3,5,6,7,9,10]. By desulfating HSPGs, it can modulate the binding of numerous signaling molecules, thereby affecting many physiological and pathological processes [5]. It is involved in pathways like TGF-β1/SMAD, FAK/PI3K/AKT/mTOR, VEGFR2/PI3K/AKT, and Wnt signaling, playing a crucial role in biological regulation [1,2,5,6,8].
In idiopathic pulmonary fibrosis, Sulf1 is upregulated in lung tissues of patients, and its knockdown in HFL1 cells suppresses fibroblast functions like secretion, activation, proliferation, migration, and invasion, suggesting it promotes fibrosis through the TGF-β1/SMAD pathway [1]. In colorectal cancer, knockdown of Sulf1 inhibits cell proliferation, invasion, and migration, while increasing apoptosis, indicating its role in promoting CRC progression via the FAK/PI3K/AKT/mTOR pathway by regulating ARSH [2]. In cervical cancer, Sulf1 promotes tumorigenesis and development by activating the VEGFR2/PI3K/AKT pathway [5]. In the context of bone metastatic prostate cancer, Sulf1 produced by bone marrow fibroblasts suppresses Wnt3A-driven growth in the desmoplastic stroma, and its loss favors cancer progression [4]. In gastric cancer, cancer-associated fibroblasts-derived Sulf1 promotes metastasis and CDDP resistance through the TGFBR3-mediated TGF-β signaling pathway [9]. In colorectal cancer, Sulf1+ cancer-associated fibroblasts enhance VEGFA release, promoting extracellular matrix deposition and angiogenesis [10].
In summary, Sulf1 is an important enzyme that modulates multiple signaling pathways. Through loss-of-function experiments in various cell lines and in some cases, by implication in disease-relevant models, it has been shown to play significant roles in fibrosis and multiple types of cancer, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Tu, Meng, Lu, Chunya, Jia, Hongxia, Yang, Ming, Zhang, Guojun. 2024. SULF1 expression is increased and promotes fibrosis through the TGF-β1/SMAD pathway in idiopathic pulmonary fibrosis. In Journal of translational medicine, 22, 885. doi:10.1186/s12967-024-05698-3. https://pubmed.ncbi.nlm.nih.gov/39354547/
2. Zhu, Wenjie, Wu, Changlei, Liu, Zitao, Cheng, Xiufeng, Huang, Jun. 2024. SULF1 regulates malignant progression of colorectal cancer by modulating ARSH via FAK/PI3K/AKT/mTOR signaling. In Cancer cell international, 24, 201. doi:10.1186/s12935-024-03383-5. https://pubmed.ncbi.nlm.nih.gov/38844922/
3. Lai, Jin-Ping, Sandhu, Dalbir S, Shire, Abdirashid M, Roberts, Lewis R. 2009. The tumor suppressor function of human sulfatase 1 (SULF1) in carcinogenesis. In Journal of gastrointestinal cancer, 39, 149-58. doi:10.1007/s12029-009-9058-y. https://pubmed.ncbi.nlm.nih.gov/19373441/
4. Brasil da Costa, Fabio Henrique, Lewis, Michael S, Truong, Anna, Carson, Daniel D, Farach-Carson, Mary C. 2020. SULF1 suppresses Wnt3A-driven growth of bone metastatic prostate cancer in perlecan-modified 3D cancer-stroma-macrophage triculture models. In PloS one, 15, e0230354. doi:10.1371/journal.pone.0230354. https://pubmed.ncbi.nlm.nih.gov/32413029/
5. Li, Juan, Wang, Xihao, Li, Zhilong, Wang, Yanyun, Xi, Mingrong. . SULF1 Activates the VEGFR2/PI3K/AKT Pathway to Promote the Development of Cervical Cancer. In Current cancer drug targets, 24, 820-834. doi:10.2174/1568009623666230804161607. https://pubmed.ncbi.nlm.nih.gov/37539927/
6. Fellgett, Simon W, Maguire, Richard J, Pownall, Mary Elizabeth. 2015. Sulf1 has ligand-dependent effects on canonical and non-canonical Wnt signalling. In Journal of cell science, 128, 1408-21. doi:10.1242/jcs.164467. https://pubmed.ncbi.nlm.nih.gov/25681501/
7. Graham, Kurtis, Murphy, Joshua I, Dhoot, Gurtej K. 2016. SULF1/SULF2 reactivation during liver damage and tumour growth. In Histochemistry and cell biology, 146, 85-97. doi:10.1007/s00418-016-1425-8. https://pubmed.ncbi.nlm.nih.gov/27013228/
8. Ji, Dong-Ni, Jin, Sai-di, Jiang, Yuan, Zhang, Ming-Yu, Xu, Chao-Qian. 2024. CircNSD1 promotes cardiac fibrosis through targeting the miR-429-3p/SULF1/Wnt/β-catenin signaling pathway. In Acta pharmacologica Sinica, 45, 2092-2106. doi:10.1038/s41401-024-01296-7. https://pubmed.ncbi.nlm.nih.gov/38760544/
9. Fang, Xingchao, Chen, Damin, Yang, Xinyu, Zhao, Siguo, Yan, Zhengyuan. 2024. Cancer associated fibroblasts-derived SULF1 promotes gastric cancer metastasis and CDDP resistance through the TGFBR3-mediated TGF-β signaling pathway. In Cell death discovery, 10, 111. doi:10.1038/s41420-024-01882-y. https://pubmed.ncbi.nlm.nih.gov/38438372/
10. Wang, Huijuan, Chen, Jiaxin, Chen, Xiaoyu, Qian, Junbin, Song, Zhangfa. . Cancer-Associated Fibroblasts Expressing Sulfatase 1 Facilitate VEGFA-Dependent Microenvironmental Remodeling to Support Colorectal Cancer. In Cancer research, 84, 3371-3387. doi:10.1158/0008-5472.CAN-23-3987. https://pubmed.ncbi.nlm.nih.gov/39250301/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen