C57BL/6JCya-Slc5a12em1flox/Cya
Common Name
Slc5a12-flox
Product ID
S-CKO-08385
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-241612-Slc5a12-B6J-VA
When using this mouse strain in a publication, please cite “Slc5a12-flox Mouse (Catalog S-CKO-08385) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Slc5a12-flox
Strain ID
CKOCMP-241612-Slc5a12-B6J-VA
Gene Name
Product ID
S-CKO-08385
Gene Alias
D630015G19, SMCT2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 2
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000045972
NCBI RefSeq
NM_001003915
Target Region
Exon 3
Size of Effective Region
~0.9 kb
Overview of Gene Research
Slc5a12, also known as sodium-coupled monocarboxylate transporter 2 (SMCT2), is a sodium-coupled low-affinity transporter for monocarboxylates. It is involved in the transport of substances like lactate, nicotinate, propionate, and butyrate, and plays a role in processes such as lactate reabsorption in the kidney, absorption of diet-derived short-chain monocarboxylates in the intestinal tract, and is associated with T-cell-related immune responses [3,1,2].
In c/ebpdelta -/- mice, where the renal expression of both Slc5a8 and Slc5a12 is almost completely ablated, there is a marked increase in urinary excretion of lactate and a decrease in blood lactate levels, providing evidence for an obligatory role of Slc5a12 in renal lactate absorption. Also, antibody-mediated blockade of SLC5A12 ameliorates disease severity in a murine model of arthritis, suggesting its role in chronic inflammatory disorders [4,1].
In conclusion, Slc5a12 is crucial for monocarboxylate transport, especially lactate handling in the kidney. Gene-knockout models like c/ebpdelta -/- mice have revealed its role in renal lactate and urate reabsorption. In addition, its blockade in murine arthritis models indicates its potential as a therapeutic target in chronic inflammatory diseases.
References：
1. Pucino, Valentina, Certo, Michelangelo, Bulusu, Vinay, Pitzalis, Costantino, Mauro, Claudio. 2019. Lactate Buildup at the Site of Chronic Inflammation Promotes Disease by Inducing CD4+ T Cell Metabolic Rewiring. In Cell metabolism, 30, 1055-1074.e8. doi:10.1016/j.cmet.2019.10.004. https://pubmed.ncbi.nlm.nih.gov/31708446/
2. Haas, Robert, Smith, Joanne, Rocher-Ros, Vidalba, Marelli-Berg, Federica M, Mauro, Claudio. 2015. Lactate Regulates Metabolic and Pro-inflammatory Circuits in Control of T Cell Migration and Effector Functions. In PLoS biology, 13, e1002202. doi:10.1371/journal.pbio.1002202. https://pubmed.ncbi.nlm.nih.gov/26181372/
3. Srinivas, Sonne R, Gopal, Elangovan, Zhuang, Lina, Ganapathy, Vadivel, Prasad, Puttur D. . Cloning and functional identification of slc5a12 as a sodium-coupled low-affinity transporter for monocarboxylates (SMCT2). In The Biochemical journal, 392, 655-64. doi:. https://pubmed.ncbi.nlm.nih.gov/16104846/
4. Thangaraju, Muthusamy, Ananth, Sudha, Martin, Pamela M, Prasad, Puttur D, Ganapathy, Vadivel. 2006. c/ebpdelta Null mouse as a model for the double knock-out of slc5a8 and slc5a12 in kidney. In The Journal of biological chemistry, 281, 26769-73. doi:. https://pubmed.ncbi.nlm.nih.gov/16873376/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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